Wyniki wyszukiwania - Biblioteka Nauki

1

Ikra - wielkie bogactwo w najmniejszym jaju

100%

Kowalska A. A. , Cejko B. I.

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nr 2[152]

2

Podstawowe wyznaczniki jakosci mlecza brzany [Barbus barbus] pozyskanego w sezonie rozrodczym po stymulacji hormonalnej za pomoca Ovopelu, Ovaprimu oraz hCG

80%

Cejko B. I. , Kucharczyk D. , Targonska K. , Glogowski J.

Komunikaty Rybackie

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2009

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nr 5

22-24

3

Impact of dibutyl phthalate and benzyl butyl phthalate on motility parameters of sperm from the European pikeperch Sander lucioperca (L.)

61%

Jarmolowicz S. , Demska-Zakes K. , Kowalski R. , Cejko B. I. , Glogowski J. , Zakes Z.

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nr 3

4

Effect of time after hormonal stimulation on semen quality indicators of common carp, Cyprinus carpio (Actinopterygii: Cypriniformes: Cyprinidae)

61%

Cejko B. I. , Kowalski R. K. , Kucharczyk D. , Zarski D. , Targonska K. , Glogowski J.

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tom 41

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nr 2

EN

Background. Obtaining the appropriate quantity of milt and spermatozoa of biologically good quality depends on a number of environmental factors. Additional factors may be involved while using a hormonal stimulation. The aim of this study was to analyse the effect of time, after stimulation with Ovopel [(D-Ala6, Pro9-NEt)-mGnRH+metoclopramide] (1 granule∙kg-1 body weight) on semen quality indicators of common carp, Cyprinus carpio L., over the period of 72 h post injection. Materials and methods. The total volume of milt (TVM, mL), volume of milt per 1 kg of the male body weight (VOM, mL∙kg-1 b.w.), total sperm production (TSP, ×109), and their concentration (×109 mL-1) in milt were determined. Additionally, the motility of spermatozoa (%) by means of the subjective method and the osmotic pressure of seminal plasma (mOsm∙kg-1) were determined. The milt was collected 24 h (group I, n = 10), 48 h (group II, n = 10), and 72 h (group III, n = 10) after stimulation with Ovopel. Results. No significant differences (P > 0.05) in the main parameters of milt i.e., the motility and concentration of spermatozoa in the milt, as well as in the osmotic pressure of seminal plasma were found between the experimental groups of the fish. Higher TSP and VOM values were recorded 24 h after Ovopel injection compared to samples obtained after 72 h (P <0.01 and P < 0.05, respectively) and 48 h (P > 0.05). TVM values were also higher at 24 h after the injection than those noted at 48 and 72 h (P < 0.01). Conclusion. The lack of significant differences in the motility and concentration of spermatozoa in milt at 24, 48, and 72 h after injection indicate that time after Ovopel administration does not have an influence on the main indicators of common carp milt quality. However, we noted significant differences in TSP, TVM, and VOM between samples obtaining 24 h and 72 h after hormonal stimulation. The highest quantity indicators i.e., the number of spermatozoa in milt and volume of obtained milt noted for samples obtained after 24 h suggest that this time is better for milt sampling than time after 48 and 72 h.

5

Osmolality of seminal plasma as an indicator of milt contamination with urine based on the exmaple of the tench Tinca tinca (L.)

61%

Cejko B. I. , Zarski D. , Targonska K. , Krejszeff S. , Kucharczyk D. , Glogowski J.

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nr 3

EN

Milt was collected from the tench Tinca tinca (L.) following hormonal stimulation with carp pituitary homogenate (CPH, group I, n = 9), Ovopel (group II, n = 8) and Ovaprim (group III, n = 9). Males non-stimulated fish were used as a control (group IV, n = 6). The parameters determined included the total volume of milt (TVM, ml) and the volume per kg of the males’ body weight (VOM, ml kg⁻¹ b.w.), total number of spermatozoa produced by the males (TSP, ×10⁹) and the number of spermatozoa per kg of their body weight (TNS, ×10⁹ kg⁻¹ b.w.). Moreover, attempts were made to show the effect of the hormone preparations on spermatozoa motility (%), their concentration in milt (×10⁹ ml⁻¹) and the total protein content in seminal plasma (mg ml⁻¹). Osmotic pressure of the seminal plasma (mOsm kg⁻¹) was determined to check if the milt samples were contaminated with urine. Pearson’s linear correlation was also determined between the osmolality, on the one hand, and the spermatozoa motility and concentration of spermatozoa in milt of the groups examined in the study, on the other. The significance of differences between the analysed parameters was checked with Tukey’s test (One-way ANOVA, α = 0.05). Motility and concentration of spermatozoa in the remained relatively low, not exceeding 22% and 5.0 · 10⁹ ml⁻¹ in each of the groups. Using CPH, Ovopel or Ovaprim did not result in any significant increase (P > 0.05) in the amount of milt obtained (TVM, VOM) or the total amount of spermatozoa produced as compared to the control group. Significant differences (P < 0.05) were found only between the TNS values for group I (CPH), and group IV (control). Osmolality of the seminal plasma did not exceed 120 mOsm kg⁻¹ in any of the groups under examination. Its low values as well as low motility and low concentration of spermatozoa in milt indicate that milt was contaminated with urine, which is also corroborated by a significant correlation between osmolality and motility of spermatozoa in group III (R² = 0.828; P < 0.001) and IV (R² = 0.983; P < 0.001) and between osmolality and concentration of spermatozoa in each of the groups (R² = 0.447; P < 0.05, group I; R² = 0.964; P < 0.001, group II; R² = 0.768; P < 0.001, group III and R² = 0.924; P < 0.001; group IV).

PL

Mlecz pozyskano od lina Tinca tinca (L.) po stymulacji hormonalnej za pomocą homogenatu przysadki mózgowej karpia (CPH, grupa I, n = 9), Ovopelu (grupa II, n = 8) oraz Ovaprimu (grupa III, n = 9). Grupę kontrolną (IV, n = 6) stanowiły samce, których nie stymulowano. Określono całkowitą objętość pozyskanego mlecza (TVM, ml) oraz objętość przypadającą na kg masy ciała samców (VOM, ml kg⁻¹ m.c.), całkowitą liczbę wyprodukowanych przez samce plemników w miliardach (TSP, ×10⁹) oraz liczbę przypadającą na kg masy ich ciała (TNS, ×10⁹ kg⁻¹ m.c.). Dodatkowo podjęto próbę wykazania wpływu zastosowanych preparatów hormonalnych na ruchliwość plemników (%) ich koncentrację w mleczu (×10⁹ ml⁻¹) oraz stężenie białka ogólnego (mg ml⁻¹) w plazmie nasienia. W celu sprawdzenia czy pobrane próby mlecza nie zostały zanieczyszczone moczem, oznaczono ciśnienie osmotyczne plazmy nasienia (mOsm kg⁻¹) oraz przeprowadzono prostoliniową korelację Pearsona między osmolalnością a ruchliwością i koncentracją plemników w mleczu badanych grup. Istotność różnic w wartościach analizowanych parametrów weryfikowano testem Tukey’a (One-way ANOVA, α = 0.05). Wartości ruchliwości i koncentracji plemników w mleczu utrzymywały się na stosunkowo niskim poziomie nieprzekraczającym 22% oraz 5.0 · 10⁹ ml⁻¹ w każdej z badanych grup. Zastosowanie CPH, Ovopelu oraz Ovaprimu nie doprowadziło do istotnego wzrostu (P > 0.05) objętości pozyskanego mlecza (TVM, VOM) oraz całkowitej ilości pozyskanych plemników (TSP) w porównaniu z grupą kontrolną. Istotne różnice (P < 0.05) stwierdzono jedynie w wartościach TNS między grupą I (CPH) a grupą IV (kontrolną). Osmolalność plazmy nasienia nie przekraczała 120 mOsm kg⁻¹ w każdej z badanych grup. Niskie jej wartości jak również wartości ruchliwości oraz koncentracji plemników w mleczu wskazują na zanieczyszczenie mlecza moczem. Stwierdzona istotna zależność między osmolalnością a ruchliwością plemników w grupie III (R² = 0.828; P < 0.001) i IV (R² = 0.983; P < 0.001) oraz między osmolalnością a koncentracją plemników w każdej z badanych grup (odpowiednio: R² = 0.447; P < 0.05, grupa I; R² = 0.964; P < 0.001, grupa II; R² = 0.768; P < 0.001, grupa III oraz R² = 0.924; P < 0.001; grupa IV) potwierdza ten fakt.

6

Influence of temperature on the effectiveness of the hormonal stimulation of male ide, Leuciscus idus (L.)

61%

Cejko B. I. , Kucharczyk D. , Targonska K. , Zarski D. , Sarosiek B. , Kowalski R. K. , Glogowski J.

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2010

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tom 18

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nr 4

7

Nasienie szczupaka (Esox lucius L.) - możliwości przechowywania plemników w warunkach chłodniczych

51%

Sarosiek B. , Cejko B. I. , Krejszeff S. , Glogowski J. , Targonska K. , Kucharczyk D. , Zarski D. , Kowalski R. K.

Komunikaty Rybackie

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2012

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nr 1

EN

One of the ways to improve methods for the artificial reproduction of fish is the possibility of storing sperm for short periods under refrigerated conditions (+4°C) without the necessity of freezing. In the experiment semen extenders with and without sugars (glucose and fructose) were applied during the storage of pike semen. The analysis of sperm motility parameters indicated that semen that had not been diluted with an immobilization buffer decreased in quality very quickly. After 24 hours of storage significant differences were noted in parameters measured with CASA, and after the subsequent two days no motile sperm were noted. The semen that was diluted with the immobilization buffer that included glucose retained 48% motile sperm after four days of the experiment. The results indicate that diluting the semen with an immobilization buffer lengthens the storage period of pike semen under refrigerated conditions.