Wyniki wyszukiwania - Biblioteka Nauki

1

Wpływ nowych technologii bim, na jakość decyzji podejmowanych przez uczestników przedsięwzięcia inwestycyjnego

100%

Mackiewicz A.

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tom nr 10

23--26

2

Recent benthic Ostracoda from Hornsund, south Spitsbergen, Svalbard Archipelago

100%

Mackiewicz A.

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nr 1

71-90

EN

This paper includes a check-list of Recent Svalbard marine ostracods based on published sources and on diploma theses as well as some new studies. This is the first study of this group of crustaceans from Hornsund. A total of 41 species belonging to 12 families were collected at 55 sampling stations from dredged sediments. Seven species are reported for the first time from the Svalbard Archipelago. Polycope orbicularis Sars is the most abundant species in the present fauna. Species compositions of Hornsund and the Liefde- fjorden are seen to have the highest similarity (S = 50.6).

3

BIM w polskim przedsiębiorstwie - zagrożenia i korzyści

88%

Mackiewicz A.

Wiadomości Projektanta Budownictwa

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2014

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tom nr 6-7

36--38

4

Advanced Therapy Medicinal Products

75%

Mackiewicz A.

|

tom 53

|

nr Supplement 1

5

Cytokiny i procesy przekazywania sygnalu: grupa cytokin typu interleukiny - 6 jako uklad modelowy

63%

Kortylewski M. , Mackiewicz A.

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nr 2

213-227

6

Wplyw uproszczen w uprawie roli pod kukurydze na wybrane wlasciwosci chemiczne gleby

63%

Drzymala S. , Mackiewicz A.

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tom 55

|

nr 2

95-101

7

Wplyw uproszczen w uprawie roli pod kukurydze na wybrane wlasciwosci fizyczne gleby

63%

Drzymala S. , Mackiewicz A.

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nr 3

83-92

8

Dantom - polska jakość sprawdzona zagranicą

63%

Mackiewicz A.

|

nr 05

9

Urządzenia BORGER w oczyszczalniach ścieków

63%

Mackiewicz A.

Ekotechnika

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2005

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tom nr 2

47-48

10

LATS1 HIPPO kinase is involved in cancer stem cell formation and EMT in melanoma

51%

Kazimierczak U. , Dondajewska E. , Zajaczkowska M. , Mackiewicz A.

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nr S2

11

Endocytosis, intracellular trafficking and degradation behavior of functionalized silk particles for targeted drug delivery

51%

Florczak A. , Mackiewicz A. , Dams-Kozlowska H.

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nr S2

12

Uziarnienie gleb wystepujacych w strefie oddzialywania kopalnictwa odkrywkowego w rejonie Konina w swietle klasyfikacji PTG, PN i FAO-USDA

51%

Drzymala S. , Kaczmarek Z. , Rybczynski P. , Mackiewicz A.

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2000

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nr 56

251-263

13

Mechanisms regulating glycosylation of human acute phase proteins

51%

Mackiewicz A. , Mackiewicz S. H. , Pawlowski T.

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nr 4

14

Cancer immunotherapy using cells modified with cytokine genes

51%

Kowalczyk D. W. , Wysocki P. J. , Mackiewicz A.

|

tom 50

|

nr 3

EN

The ability of various cytokines to hamper tumor growth or to induce anti-tumor immune response has resulted in their study as antitumor agents in gene therapy approaches. In this review we will concentrate on the costimulation of antitumor immune responses using modification of various cell types by cytokine genes. Several strategies have emerged such as (i) modification of tumor cells with cytokine genes ex vivo (whole tumor cell vaccines), (ii) ex vivo modification of other cell types for cytokine gene delivery, (iii) delivery of cytokine genes into tumor microenvironment in vivo, (iv) modification of dendritic cells with cytokine genes ex vivo. Originally single cytokine genes were used. Subsequently, multiple cytokine genes were applied simultaneously, or in combination with other factors such as chemokines, membrane bound co-stimulatory molecules, or tumor associated antigens. In this review we discuss these strategies and their use in cancer treatment as well as the promises and limitations of cytokine based cancer gene therapy. Clinical trials, including our own experience, employing the above strategies are discussed.

15

Expression of soluble recombinant TGF-beta type II receptor fused with the Fc portion of human IgG1 [sTbetaRII-Fc] in NS0 cells

51%

Kwiatkowska E. P. , Kazimierczak U. , Mackiewicz A. , Kowalczyk D. W.

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tom 53

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nr 2

EN

We have constructed and expressed recombinant chimeric soluble TGF-β type II receptor fused with the Fc portion of human IgG1 (sTβRII-Fc) in NS0 mouse myeloma cells and isolated cell lines constitutively secreting very high levels of biologically active protein. The GS-NS0 expression system takes advantage of the strong human cytomegalovirus immediate early promoter expression vector and glutamine synthetase as a selectable marker. The recombinant chimeric receptor could be produced in high amounts and efficiently purified by one step chromatography on a protein A column. Biochemical studies revealed that recombinant sTβRII-Fc binds native TGF-β1 and TGF-β3 isoforms and neutralizes their activity in vitro.

16

The CDKN2a common variants: 148 Ala-Thr and 500 C-G in 3' UTR, and their association with clinical course of melanoma

51%

Lamperska K. M. , Przybyla A. , Kycler W. , Mackiewicz A.

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tom 54

|

nr 1

EN

Changes in CDKN2a gene are known to be linked with sporadic melanoma and hereditary predisposition to this cancer. In the Polish population mutations in the coding region of the CDKN2a gene are rather rare, therefore the attention has been focused on polymorphisms and alterations in uncoding regions such as 3' UTR. The aim of this study was to analyze two common polymorphisms, Ala148Thr and 500 C/G, and correlate them with the clinical course of melanoma. DNA from 285 patients was analyzed and found polymorphisms were correlated with the clinical parameters employing statistical methods. The obtained results allow us to conclude: (i) survival times of 500 C/G carriers vs. cumulating proportion surviving was not statistically significant; (ii) CDKN2a polymorphism 500 C/G correlated with Ala148Thr; (iii) no correlation was observed between the 500 C/G polymorphism and age of diagnosis, localization of primary melanoma and survival time; (iv) we did not find correlation between 500 C/G and type of cancer in the family; (v) changes in the CDKN2a gene were not found in patients with second cancer.

17

Numerical analysis of deformation and flow in the proximal area of the urethra

38%

Rudyk R. , Malinowski M. , Mackiewicz A. , Bedzinski R. , Noszczyk-Nowak A. , Skonieczna J. , Madej J. P.

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tom Vol. 25, no. 2

130--141

EN

Pathological conditions of a male urethra, including fibrosis, have a mechanical background along the entire length of the urethra. They may be caused by excessive deformation of the urethra locally or globally. The condition of prolonged overload causes abnormal tissue remodelling and, consequently, the formation of a thick layer of scar tissue differentiated from the connective tissue of the urethra. This tissue, which has higher mechanical properties, is not highly deformable and therefore, causes a decrease in the diameter of the urethra, which results in conditions that disturb the natural flow of urine. In this paper, it was decided to determine the deformation conditions in the proximal part of the urethra. The study was conducted in three main stages. Transverse sections of the animal urethral tissues were prepared in order to examine mechanical properties and perform histological examinations. On the basis of these examinations, material models which fitted best for the experimental results were sought. Material constants of the Mooney-Rivlin material model with the best fit ratio were determined for further research. On the basis of histological photographs, a geometrical and numerical model of the urethra was developed. The urethra was tested in a flat state of deformation. The strain and stress fields of the Caucha tensor were examined. The methodology of testing the dynamics of the urine flow in the highly deformable urethra was proposed. This is important for the analysis of the influence of at excessive pressure on pathological tissue remodelling leading to fibrosis.

18

Expression of p16 in sporadic primary uveal melanoma

32%

Lamperska K. , Mackiewicz K. , Kaczmarek A. , Kwiatkowska E. , Starzycka M. , Romanowska B. , Heizman J. , Stachura J. , Mackiewicz A.

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nr 2

EN

Expression of p16 protein, intragenic mutations of CDKN2A and hypermethylation of CDKN2A promoter region in 41 sporadic primary uveal melanomas were studied. There were 2 cases of spindle cell B histological type, 11 of A + B and 28 of mixed type. All melanomas infiltrated sclera but in 28 cases infiltration was superficial while in 13 profound. In 7 cases the tumor infiltrated the optic nerve. Expression of p16 was studied by immunohistochemistry and recorded by assessment of the proportion of positive tumor cells and staining intensity. Results were expressed as staining index (IRS). Intragenic mutations were studied by PCR-SSCP followed by sequencing, while hypermethylation of the promoter region by CpG methylation assay. In 15% of cases less than 10% of melanoma cells were p16 positive, in 70% of cases less than 50% of cells, while in 7% more than 80% of cells stained for p16 (mean IRS for all cases was 4.87 + 2.43). In B type the IRS was 8.5 + 0.7, in A + B type 6.0 + 2.1 and in the mixed type 4.17 + 2.43 (differences statistically significant). In melanomas profoundly infiltrating sclera mean IRS was 4.16, while in those infiltrating optic nerve 3.71 (statistically not significant). Analysis of the intragenic mutations revealed in two patients a GAC/GAT substitution in codon 84 — a silent mutation. No hypermethylation of the CpG island of the p16 promoter region was found. In conclusion, we found that the degree of p16 expression is related to the histological type of tumor but not to the histological indicators of tumor invasiveness and that intragenic mutations and promoter hypermethylation are not major mechanisms of p16 inactivation in sporadic uveal melanoma.

19

Human melanoma gene therapy: from animal studies to clinical trials

26%

Mackiewicz A. , Kapcinska M. , Laciak M. , Malicki J. , Murawa P. , Nowak J. , Sibilska E. , Wiznerowicz M. , Breborowicz D. , Lange A. , Hawley R. G. , Heinrich P. C. , Rose-John S.

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nr 4