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2 Biotechnologia

2 2003

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Gelatin sponge Spongostan as a carrier for animal and human adherent cell culture

100%

Paduch R. , Rzeski W. , Zdzisinska B. , Kaczor J. , Kandefer-Szerszen M.

2003

nr 1

223-233

A porous gelatin sponge Spongostan (Ferrosan Denmark) as a carrier for the cultivation of several adherent cell lines and strains was tested. This material provide considerably greater surface available for cells than conventional bottles. Moreover, it allows to maintain high cell viability (over 87%) during culture time. When cells were cultured on Spongostan discs (wet vol. 0.11 cm3), no phenotypic changes in cell adherence and proliferation dynamics were observed. However, when cells adhered to Spongostan were frozen down and kept for 5 months in liquid nitrogen, they lost about 65% of their viability. Generally, Spongostan can be considered as a universal carrier for three-dimensional culture of many cell types, but it cannot be used for cells? freezing.

Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor

72%

Rzeski W. , Paduch R. , Kozdrun W. , KaczorJ. , Czekaj H. , Samorek-Salamonowicz E. , Kandefer-Szerszen M. , Zdzisinska B. , Dawidowicz A.

2003

nr 2

261-267

Derzsy desease is a fatal hepatitis of young geese under the age of 5 weeks. The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is the only method preventing the loss of animals. In vitro GPV replicates only in goose embryo fibroblasts (GEF) and this way the virus is propagated in order to obtain the material for diagnostic tests and vaccine production. In this paper, we report the application of bioreactor Celligen Plus for GEF culture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing to the traditional cell culture techniques, the described method was over 12-fold more efficient and allowed to obtain good quality virus pool for Derzsy disease vaccine preparation.