Wyniki wyszukiwania - Biblioteka Nauki

1

Analyzing photosynthetic activity and growth of Soanum lycopersicum seedlings exposed to different light qualities

100%

Wu Q. , Su N. , Shen W. , Cui J.

Acta Physiologiae Plantarum

|

2014

|

tom 36

|

nr 06

EN

To investigate how light quality influences tomato (Solanum lycopersicum L) seedlings, we examined changes in plant growth, chloroplast ultrastructure, photosynthetic parameters and some photosynthesis-related genes expression levels. For this, tomato plants were grown under different light qualities with the same photosynthetic photon flux density: red (R), blue (B), yellow (Y), green (G) and white (W) lights. Our results revealed that, compared with plants grown under W light, the growth of plants grown under monochromatic lights was inhibited with the growth reduction being more significant in the plants grown under Y and G lights. However, the monochromatic lights had their own effects on the growth and photosynthetic function of tomato seedlings. The plant height was reduced under blue light, but expression of rbcS, rbcL, psbA, psbB genes was up-regulated, and the ΦPSII and electron transport rate (ETR) values were enhanced. More starch grains were accumulated in chloroplasts. The root elongation, net photosynthetic rate (Pn), NPQ and rbcS and psbA genes expression were promoted under red light. Yellow light- and green light-illuminated plants grew badly with their lower Rubisco content and Pn value observed, and less starch grains accumulated in chloroplast. However, less influence was noted of light quality on chloroplast structure. Compared with yellow light, the values of ΦPSII, ETR, qP and NPQ of plants exposed to green light were significantly increased, suggesting that green light was beneficial to both the development of photosynthetic apparatus to some extent.

2

Changes in the polarization states of random electromagnetic vortex beams propagating in biological tissues

100%

Duan M. , Wu Y. , Su N.

Optica Applicata

|

2018

|

tom Vol. 48, nr 2

297--309

EN

The changes in the on-axis polarization state of random electromagnetic Gaussian Schell-model vortex beams propagating in biological tissues have been studied. In different media propagation, the bigger Cn2 is, the earlier the appearance of the inflexion points in the on-axis degree of the polarization P(0, 0, z) is. As the propagation distance increases, the values of the on-axis orientation angle θ(0, 0, z) undergo several processes: at the beginning they are positive, then gradually increase to the maximum, jump to a negative value, finally tend to a fixed value. The bigger Cn2 corresponds to previous jumping position. In the entire propagation, the values of the on-axis ellipticity ε(0, 0, z) are larger than the initial one. There exists a phenomenon that the values of P(0, 0, z), θ(0, 0, z) and ε(0, 0, z) keep their extremes in a length of propagation distances for the far infrared beams. The maximum of P(0, 0, z) is the smallest and the jumping range of θ(0, 0, z) is the largest for the ultraviolet beams. Compared with σyy > σxx, the changes in magnitudes in P(0, 0, z) are more obvious when σyy < σxx, the changes in θ(0, 0, z) are just the reverse for σyy < σxx.

3

Gene expression patterns in bone following lipopolysaccharide stimulation

100%

Yang J. , Su N. , Du X. , Chen L.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

|

nr 4

EN

Bone displays suppressed osteogenesis in inflammatory diseases such as sepsis and rheumatoid arthritis. However, the underlying mechanisms have not yet been clearly explained. To identify the gene expression patterns in the bone, we performed Affymetrix Mouse Genome 430 2.0 Array with RNA isolated from mouse femurs 4 h after lipopolysaccharide (LPS) administration. The gene expressions were confirmed with real-time PCR. The serum concentration of the N-terminal propeptide of type I collagen (PINP), a bone-formation marker, was determined using ELISA. A total of 1003 transcripts were upregulated and 159 transcripts were downregulated (more than twofold upregulation or downregulation). Increased expression levels of the inflammation-related genes interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) were confirmed from in the period 4 h to 72 h after LPS administration using real-time PCR. Gene ontogene analysis found four bone-related categories involved in four biological processes: system development, osteoclast differentiation, ossification and bone development. These processes involved 25 upregulated genes. In the KEGG database, we further analyzed the transforming growth factor β (TGF-β) pathway, which is strongly related to osteogenesis. The upregulated bone morphogenetic protein 2 (BMP2) and downregulated inhibitor of DNA binding 4 (Id4) expressions were further confirmed by real-time PCR after LPS stimulation. The osteoblast function was determined through examination of the expression levels of core binding factor 1 (Cbfa1) and osteocalcin (OC) in bone tissues and serum PINP from 4 h to 72 h after LPS administration. The expressions of OC and Cbfa1 decreased 6 h after administration (p < 0.05). Significantly suppressed PINP levels were observed in the later stage (from 8 h to 72 h, p < 0.05) but not in the early stage (4 h or 6 h, p > 0.05) of LPS stimulation. The results of this study suggest that LPS induces elevated expressions of skeletal system development- and osteoclast differentiation-related genes and inflammation genes at an early stage in the bone. The perturbed functions of these two groups of genes may lead to a faint change in osteogenesis at an early stage of LPS stimulation. Suppressed bone formation was found at later stages in response to LPS stimulation.

4

Genetic diversity in Salix gordejevii populations from different environmental gradients in Horqin Sandy Land, Northern China

75%

Huang W.-D. , Zhao X.-Y. , Zhao X. , Luo Y.-Y. , Feng J. , Su N. , He Y.-Z.

Polish Journal of Ecology

|

2019

|

tom 67

|

nr 3