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EN
This study presents root colonization of Deschampsia cespitosa growing in the immediate vicinity of a former Pb/Zn smelter by arbuscular mycorhizal fungi (AMF) and dark septated endophytes (DSE) at different soil depths. AMF spores and species distribution in soil profile were also assessed. Arbuscular mycorrhiza (AM) and DSE were found in D. cespitosa roots at all investigated soil levels. However, mycorrhizal colonization in topsoil was extremely low with sporadically occurring arbuscules. AM parameters: frequency of mycorrhization of root fragments (F%), intensity of root cortex colonization (M%), intensity of colonization within individual mycorrhizal roots (m%), and arbuscule abundance in the root system (A%) were markedly higher at 20–40, 40–60 cm soil levels and differed in a statistically significant manner from AM parameters from 0–10 and 10–20 cm layers. Mycorrhizal colonization was negatively correlated with bioavailable Cd, Pb and Zn concentrations. The number of AMF spores in topsoil was very low and increased with soil depth (20–40 and 40–60 cm). At the study area spores of three morphologically distinctive AMF species were found: Archaeospora trappei, Funneliformis mosseae and Scutellospora dipurpurescens. The fourth species Glomus tenue colonized roots of D. cespitosa and was observed in the root cortex at 20–40 and 40–60 soil depth, however, its spores were not found at the site.
PL
Określono status mikoryzowy Miscanthus х gigantem Greef et Deuter - azjatyckiego gatunku wieloletniej trawy uprawianej obecnie do celów energetycznych. Miskanta uprawiano na terenie zanieczyszczonym Cd, Zn i Pb w Bytomiu oraz na poletku kontrolnym w Katowicach. Na wszystkich poletkach badawczych w korzeniach miskanta stwierdzono obecność struktur typowych dla mikoryzy arbuskularnej. Nie zaobserwowano różnic w poziomie kolonizacji mikoryzowej pomiędzy poletkiem kontrolnym a poletkami zanieczyszczonymi metalami. Kumulacja Cd, Zn i Pb w pędach i korzeniach miskanta rosła wraz z ich całkowitą zawartością w glebie. W większości przypadków wyższe zawartości metali odnotowywano w korzeniach niż w pędach.
EN
Miscanthus х giganteus Greef et Deuter, is a large bunch grass, originating from Asia, which have the potential for very high rates of growth. Miscanthus crops could be successfully grown on contaminated land. Although some Miscanthus species e.g. M. sinensis and M. florodulus were described as mycorrhizal [Ma et al. 2006; Leung et al. 2007] the present investigation is the first one to demonstrate the characteristic of Miscanthus х giganteus mycorrhiza from an area contaminated with Cd, Zn, and Pb. Roots for mycorrhizal studies were collected from contaminated site in Bytom and from control non-contaminated plot in Katowice. Metal concentrations in soil and plants roots and shoots were also examined Arbuscular mycorrhizal structures were described in plant from both heavy metal polluted and control plots. Mycorrhizal colonization level of Miscanthus х giganteus in the place with the highest content of metals in soils was not different from that observed in the control plot. Cd and Zn were mostly accumulated in the roots but by contrast Pb concentrations were found to be higher in shoots. Our results clearly demonstrated that alien for Polish flora grass species Miscanthus х gigantem really formed arbuscular mycorrhizal association in soil contaminated with of Cd, Zn and Pb.
EN
We analyzed concentrations of Cd, Cu, Fe, Pb, and Zn in bark, leaves, young shoots, pericarp, and seeds of Robinia pseudoacacia from several sites (three industrial cities and two rural villages) in southern Poland. The objective of the study was to determine the possible usefulness of various organs and tissues of this species for biomonitoring. The analyses showed that bark and leaves are organs that may serve as good bioindicators. The lowest concentrations of Cd, Pb, and Zn were found in one of the industrial cities in the area. On the other hand, the concentrations of Cd, Pb, and Zn were similar in the rural areas when compared to the concentrations observed in the cities. The high level of metal contamination of air in rural areas may be a result of the long-range transport of emissions. Also, the use of bark and leaves of R. pseudoacacia enables us to estimate the level of contamination by Fe and Cu of areas in the vicinity of railway tracks. On the basis of the data presented in this paper and the data from references, a conclusion can be drawn that the bark and leaves of R. pseudoacacia are good bioindicators of metal contamination of air in towns andcities with different traffic intensities and within the surroundings of industrial plants and railway tracks. R. pseudoacacia was also used for the first time to assess the long-range transport of emissions.
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