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Isolation and expression analysis of miR165a and REVOLUTA from Brassica species

100%

Kumari G. , Kusumanjali K. , Srivastava P. S. , Das S.

Acta Physiologiae Plantarum

2013

tom 35

nr 02

The present study was performed to survey the nature and extent of sequence conservation and divergence of miR165a and its target REVOLUTA (REV), a HD-ZIP III family member from Brassica encompassing both diploid and allotetraploid genomes. Post-transcriptional gene silencing (PTGS)-based regulation in plant requires precise pairing of the miRNA–mRNA target. We identified length and sequence polymorphisms in pre-miR165a, although 21 nt mature sequence was largely invariant. One Single Nucleotide Polymorphism (SNP) each in mature miR165a that has the potential to disrupt PTGS was detected in one clone each from B. rapa var. Chinese Cabbage and B. oleracea var. Brussels Sprout. The miR165 binding site in REV in Brassica species is split between exons 4 and 5 and is reconstituted in the mRNA with no sequence variation. In REV, allelic variation can be observed in the flanking exonic and intronic regions in both diploid and allopolyploid species of Brassica indicating a strong selection pressure for maintaining the miR165a target site in REV such that deleterious mutation at the site of PTGS does not accumulate in the population. In addition, the present study indicates that miR165a is expressed in organ-specific manner and regulates its target transcript level through PTGS mechanism.

Postseismic deformation of a layered half-space due to a very long dip-slip fault

100%

Singh S. , Kumari G. , Singh M.

Acta Geophysica Polonica

2000

tom Vol. 48, nr 1

69-77

The relaxed rigidity method is used for calculating the postseismic deformation of an elastic layer (lithosphere) overlying a yiscoelastic half-space (asthenosphere) caused by a very long dip-slip fault in the layer. The integrals involved are evaluated approximately by replacing the integrand by a finite sum of exponential terms. Detailed numerical results showing the variation of the surface displacements with the epicentral distance for various source depths and for various values of the relaxed rigidity of the half-space are presented graphically.