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1
Anti-oxidant enzymes of Acanthamoeba castellanii strains
100%
Hadas E.
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nr 2
2
Content available Prostaglandyny pasożytów i hipotezy ich działania na organizm żywiciela
100%
Hadas E.
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nr 4-6
3
Biosynthesis of prostaglandins in pathogenic and non-pathogenic strains of Acanthamoeba castellanii
100%
Hadas E.
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nr 1
4
Factors conditioning biosynthesis of prostaglandins in pathogenic and non-pathogenic strains of Acanthamoeba castellanii
100%
Hadas E.
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tom 27
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nr 3-4
5
Histochemical investigation of the synthetase of prostaglandin in pathogenic and non-pathogenic strains of Acanthamoeba castellanii and infected host tissue
100%
Hadas E.
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nr 2
6
Histochemical investigations of prostaglandin synthetase in Trichinella spiralis larvae
100%
Hadas E.
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nr 1
EN
The target of the study was to examine histochemically in vivo the activity of prostaglandin synthetase in Trichinella spiralis larvae in stages of their migration, settlement and encapsulation in the host’s tissues. It was proved that prostaglandins may play certain role in producing circumlarval capsules.
7
Prostaglandins of the larvae of Trichinella spiralis and T. pseudospiralis
63%
Hadas E. , Staude-Adamczewska M.
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nr 3
8
Content available Porównawcze badania biochemiczne patogenicznych i niepatogenicznych pełzaków z rodzaju Naegleria
63%
Hadas E. , Kasprzak W.
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tom 33
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nr 1
9
Content available Wykorzystanie obecnosci celulozy w scianie komorkowej cyst Acanthamoeba do celow diagnostycznych
63%
Derba M. , Hadas E.
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nr 1
47-51
10
Content available Wolnorodnikowe mechanizmy biochemicznej obrony zywiciela
63%
Boczon K. , Hadas E.
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nr 2
169-174
EN
The mechanism of intracellular killing of pathogens which involves both oxygen and reactive nitrogen intermediates have been discussed. The attention is focused on inducible nitric oxide synthase which produces NO, a soluble free radical. The role of nitric synthase in the pathogenesis of many parasitic infections has recently been emphasised. The participation of enzymatic and non-enzymatic antioxidants in the pathogenesis of many infections and the role of proteasomes in the process of repair of the oxidetively damaged proteins are also discussed.
11
Content available Wykorzystanie obecnosci celulozy w scianie komorkowej cyst Acanthamoeba do celow diagnostycznych i terapeutycznych
63%
Derda M. , Hadas E.
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tom 53
178
12
Field studies of immunisation of 3-4 months old lambs with drug-abbreviated infections
63%
Hadas E. , Stankiewicz M.
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nr 1
EN
We investigated the ability of young lambs to develop protective immunity to gastro-intestinal nematode following immunisation with drug-abbreviated infection. Thirty, 3-4 month old lambs were randomly allocated to five groups. Groups 1, 2 and 3 were immunised by three Oxfendazole-abbreviated artificial infections of Trichostrongylus colubriformis and Ostertagia circumcincta. Group 1 received three high immunising larval doses. Groups 2 and 3 were immunised in the same way as Group 1 but with 50 and 75% of the larval dose, respectively. Group 4 was treated with Oxfendazole at the same time as Groups 1, 2 and 3, while Group 5 was an untreated control. All groups grazed the same pasture. At the end of experiment the lambs from Groups 1 and 4 had higher liveweight gains and a higher dag weights than Group 5. Mean total faecal worm egg count was reduced in animals from Groups 1-4, compared controls. However, because of the limited efficacy of vaccination of 3-4 month old lambs by drug-abbreviated infections we suggest that immunisation of young lambs may not be practical unless accompanied by appropriate immunostimulation of the gut mucosa.
13
Antioxidants and proteolytic enzymes in experimental trichinellosis
63%
Derda M. , Hadas E.
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nr 4
EN
Antioxidant and proteolytic enzymes play an important role during trichinellosis. In the present study we show a statistically significant increase in the enzymatic activity of both enzymatic superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and non-enzymatic total antioxidant status (TAS) occurs in the muscles of mice infected with Trichinella spiralis and T. pseudospiralis. Our research has shown that SOD and catalase activity increase during the 1st week of trichinellosis and remain at an elevated level for at least 10-15 weeks following the infection. The activity of proteolytic enzymes is correlated with intensification of antioxidant activity. This activity disappears gradually and simultaneously with the decrease in the activity of antioxidative enzymes induced by T. spiralis. In the T. pseudospiralis infection, proteolytic activity persist longer together with SOD and catalase activity. Upon comparison of the infection induced by T. spiralis and by T. pseudospiralis it was established that the maximum increase in the activity of all analysed enzymes occurs much earlier in the infection with T. spiralis.
14
The effect of anthelmintic treatment or immunisation of ewes during pregnancy on the periparturient rise in nematode parasite eggs excreted by ewes and on live-weight gain of lambs
63%
Stankiewicz M. , Hadas E.
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nr 1
EN
As immunisation of young lambs against parasitic nematodes is not currently feasible, we were interested to know whether drug treatment or immunisation of pregnant ewes on pasture with drug-abbreviated infections during pregnancy would have practical implications on the periparturient rise in excretion of parasite eggs by ewes and live-weight gain in lambs born to these ewes. Romney 4-year-old ewes were immunised with three (group 1), two (group 2) and one (group 3) immunising doses of Ostertagia circumcincta together with Trichostrongylus colubriformis. Group 4 sheep were treated with oxfendazole only. Group 5 sheep served as an untreated controls and non- lactating sheep (group 6) were used as additional controls. These results show that periparturient rise in parasite eggs was in the 4-year-old ewes moderate, but significantly decreased either by drug treatment or by drug-abbreviated immunisation. There was a tendency for lambs born from immunised ewes to gain weight more rapidly than lambs born from non-immunised ewes.
15
Content available Wykrywanie obecnosci Acanthamoeba sp.w probach srodowiskowych i materiale pochodzacym od pacjentow przy pomocy techniki PCR
51%
Derda M. , Hadas E. , Sulek A.
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nr 4
747
16
Biochemical changes of Acanthamoeba following attenuation and the role of cysts in retaining the characteristics of srains
51%
Kasprzak W. , Mazur T. , Hadas E.
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nr 4
17
Content available Biochemical aspects of the host defence mechanisms in experimental trichinellosis
51%
Boczon K. , Hadas E. , Derda M.
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nr 3
18
Content available Extracerebral infections of Acanthamoeba spp. in mice. Pathomorphological changes in tissues of infected animals
51%
Mazur T. , Gustowska L. , Hadas E.
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nr 1
EN
A variety of Acanthamoeba spp. pathogenic strains were isolated from water pools in the area of Poznań. In many cases amoebae invaded lugs, kidney heart, liver and diaphragm causing significant changes in these Organs. The presence of amoeba outside of CNS was found mainly in the early periods of infection. Amoeba isolated from different organs of dead animals did not exhibit any specificity in relation to the organs in following infections. Infection with both low-and high-virulent strains resulted in pathomorphological changes of similar character. However, changes induced by low-virulent parasites developed later and were less extensive.
19
Plant extracts as amoebicidal drugs in acanthamebiasis
45%
Derda M. , Hadas E. , Nowak G. , Thiem B. , Wojt W. J.
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nr Suppl.
20
The effect of methylprednisolone on the dynamics of oxygen uptake and peroxidase and superoxide dismutase activities in skeletal muscles of mice infected with Trichinella spiralis larvae
45%
Wandurska-Nowak E. , Boczon K. , Derda M. , Hadas E. , Matias-Guzek A.
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nr 1
EN
The present study was undertaken to examine whether treatment with methylprednisolone could normalise (modulate) the biochemical dysfunctions in the skeletal muscles of mice in experimental trichinellosis. Our studies included investigations of two indices of the uncoupling of oxidative phosphorylation: RCI value (respiratory control index) and QO₂ (oxygen uptake in state IV and in state III) as well as of two antioxidant enzyme activities: peroxidase (POX, E.C. 1.11.1.7) and superoxide dismutase (SOD, E.C. 1.15.1.1) in mouse muscles infected with Trichinella spiralis. Treatment with methylprednisolone was responsible for the normalisation of the activity of POX at 30 d.p.i. and was responsible for 50% decrease in stimulation of SOD activity at 43 d.p.i. At other points of measurement the drug showed no statistical influence on the activities of this enzymes. The drug had not statistically significant effect on the value of the respiratory control index and on the value of oxygen uptake.
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