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3 Bulletin of the Veterinary Institute in Puławy

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Immune responses induced in hens after oral administration of bovine serum albumin in combination with an extract of Uncaria tomentosa

100%

Jonauskiene I. , Bizanov G.

tom 52

nr 4

A dry extract from the bark of Uncaria tomentosa was evaluated as a potential immunomodulator in various formulations. Hens were immunised three times orally with a bovine serum albumin (BSA) supplemented with 7, 70, and 280 mg of the dry extract of the bark. Specific IgA antibodies in saliva and IgY antibodies in yolk were examined by ELISA. It was found that the birds inoculated with BSA in addition to 280 mg of Uncaria tomentosa bark extract had a higher amount of BSA-specific IgA antibodies in the saliva and BSA-specific IgY antibodies in yolk, than hens that received lower doses of the extract. Furthermore, the hens immunised with BSA alone had significantly lower immune responses to BSA than hens immunised with BSA supplemented with the bark extract. These results suggest that dry extract from bark of Uncaria tomentosa is useful as an oral adjuvant or immunomodulator for hens.

Mucosal and humoral immunity in mice immunized intranasally with murine parainfluenza virus type 1 in combination with adjuvants

100%

Bizanov G. , Jonauskiene I.

nr 2

Humoral response to recombinant human growth hormones administered to rabbits alone and in combination with Stabileze 06

80%

Martisius B. N. , Bizanov G. , Mauricas M. , Jonauskiene I.

nr 4

The effects of intramuscular immunisation of rabbits with recombinant human growth hormone (rhGH) combined with Stabileze 06 (methylvinylether/maleic anhydride copolymer crosslinked with 1,9-decadiene) or alone was investigated. Specific IgG and IgA antibodies in serum were examined by an ELISA. High titres of IgG antibodies against rhGH were recorded in the animals administered with the hormone in combination with Stabileze 06. The present results show that a formulation containing entrapped recombinant protein in viscous semi-stable emulsion Stabileze 06 may increase an immune response in rabbits.