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3 Acta Biochimica Polonica

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Solubilization and one-step purification of mannosylphosphodolichol synthase from Trichoderma reesei

100%

Kruszewska J. S. , Perlinska-Lenart U. , Palamarczyk G.

nr 2

Mannosylphosphodolichol synthase (MPD-synthase) (EC 2.4.1.830) catalyzing formation of MPD from GDPMan and dolichylphosphate (PD) has been purified from T. reesei cellular membranes almost to homogeneity. Selective solubilization of the enzyme was followed by one step purification on Phenyl-Sepharose column. SDS/ PAGE of the purified enzyme fraction revealed the presence of a protein band of 31 kDa corresponding to the apparent molecular mass of the MPD-synthase purified from S. cerevisiae [Babczinski, P. et al. (1980) Eur. J. Biochem. 105,509-515; Haselbeck A. (1989) Eur. /. Biochem. 181, 663-6681. During solubilization, the enzyme was stabilized by the presence of a lipophilic substrate dolichylphosphate and phospholipids as well as by protease inhibitors. The Phenyl-Sepharose purified enzyme had an absolute requirement for dolichylphosphate and was activated by cAMP dependent protein kinase.

Molecular characterization of central cytoplasmic loop in Aspergillus nidulans AstA transporter

76%

Pilsyk S. , Sienko M. , Brzywczy J. , Golan M. P. , Perlinska-Lenart U. , Kruszewska J. S.

2018

tom 65

nr 4

Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition

76%

Gorka-Niec W. , Pniewski M. , Kania A. , Perlinska-Lenart U. , Palamarczyk G. , Kruszewska J. S.

nr 2

251-259

In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1gene in Trichodermacaused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.