Wyniki wyszukiwania - Biblioteka Nauki

1

Nowa choroba wirusowa - duzym zagrozeniem dla chowu karpia w Polsce

100%

Sadowski J. , Kempter J.

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nr 3

35-38

2

Eel species identification by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP)

80%

Keszka S. , Panicz R. , Kempter J.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 05

EN

Eels offered on the Polish market are not only imported mainly from China but also from domestic catches. It is known that Chinese breeders are buying most of their montče eels from Europe, so it is highly probable that “Chinese” eels are Anguilla anguilla, but also Anguilla japonica. There is no data available concerning ratio between these two species on the Polish market. Morphological methods applied to establish this ratio are not reliable enough. Therefore the aim of the presently reported study was to differentiate the eel species using molecular methods. A total of 31 freshwater eels were collected from a local importer (21 samples) and from Lake Miedwie near Szczecin (10 samples). At the beginning of the eel identification process morphometric measurements have been performed. In attempting to distinguish A. japonica and A. anguilla PCR products of partial 16S rRNA gene, a PCR-RFLP procedure was applied, which is mainly base on nucleotide differences between species sequences. In this method the ApaI restriction enzyme was used to conduct the digestion of the PCR product. Primers named Ang211F and Ang211R were designed for the amplification the 211 bp of 16S rRNA sequence of both eel species. Electrophoretic pattern of PCR products from A. japonica and A. anguilla did not indicate any difference in length. As a result, ApaI produced fragments of 135 and 76 bp only for A. japonica, while the A. anguilla sequence was not digested with its length of 211 bp. Products of ApaI digestion of partial 16S rRNA gene of A. japonica and A. anguilla are suitable genetic markers to distinguish both eel species.

3

Microsatellite DNA-based genetic traceability of two populations of splendid alfonsino, Beryx splendens (Actinopterygii: Beryciformes: Berycidae)—Project CELFISH—Part 2

80%

Kempter J. , Kielpinski M. , Panicz R. , Keszka S.

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2016

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tom 46

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nr 4

4

Presence of Herpesvirus anguillae (AngHV1) DNA in the native ichthyofauna of North-Western Poland

80%

Tuan N. T. , Kempter J. , Panicz R.

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tom 19

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nr 4

5

A new monogenean species parasitic on gills of bigmouth sole, Hippoglossina stomata Steindachner, 1876 from Southern California

80%

Piasecki W. , Wierzbicka J. , Kempter J.

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tom 30

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nr 1

EN

A new monogenean species Neoheterobothrium hippoglossini sp. n. from the gill cavity of Hippoglossina stomata Steindachner, 1876 is described and illustrated. The new species differs from its seven congeners in a number of morphological features and in having a different host. The validity of the genus Neoheterobothrium is discussed. Neoheterobothrium mcdonaldi Payne, 1987 is relegated from the genus and from the subfamily Choricotylinae and placed in one of the remaining subfamilies of the family Diclidophoridae.

PL

W jamie skrzelowej soli wielkogębowej Hippoglossina stomata Steindachner, 1876 złowionej w Pacyfiku, przy brzegu w okolicy miejscowości Huntington Beach znaleziono pięć osobników przywry monogenetycznej. Szczegółowe badania morfologiczne wykazały, że znaleziony pasożyt reprezentuje rodzaj Neoheterobothrium Price, 1943. Badane osobniki różniły się pod względem niektórych cech morfologicznych od innych gatunków należących do tego rodzaju, dlatego też autorzy zdecydowali się uznać go za nowy gatunek, nadając mu nazwę Neoheterobothrium hippo- glossini sp. n. Niniejsza praca zawiera dyskusję dotyczącą rodzaju Neoheterobothrium. W wyniku tej dyskusji Neoheterobothrium mcdonaldi Payne, 1987, został usunięty z rodzaju oraz podrodziny Choricotylinae dojednej z pozostałych podrodzin rodziny Diclidophoridae.

6

Genetic traceability of selected populations of the yellowstripe scad, Selaroides leptolepis (Actinopterygii:Perciformes:Carangidae), based on the analysis of microsatellite DNA-CELFISH Project-Part 3

71%

Kempter J. , Kielpinski M. , Panicz R. , Mikolajczyk K. , Keszka S.

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nr 3

7

Genetic variability in European populations of Coregonus lavaretus (L.): an assessment based on mitochondrial ND-1 gene haplotypes

71%

Kempter J. , Kohlmann K. , Panicz R. , Sadowski J. , Keszka S.

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2010

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tom 18

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nr 4

8

PCR-RFLP-based molecular studies on inter-population variability in the crucian carp (Carassius carassius L.)

61%

Kempter J. , Panicz R. , Makowska M. , Metza M. , Keszka S. , Kielpinski M. , Sadowski J.

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tom 18

EN

Genetic variability of three sympatric crucian carp ( Carassius carassius ) populations from NW Poland was studied within a research project aimed at assessing the utility of those populations for stocking in inland waters. DNA samples were collected from 65 individuals. Restriction analysis was performed using 4 enzymes (HaeIII, HinfI, FspBI, TasI) of known restriction sites. The restriction pro files obtained were classified as belonging to a single haplotype (H-1). Selected DNA products were sequenced; the subsequent comparison made it possible to detect the presence of substi- tutions in the genome fragment analysed.