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tom 64
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nr 07
900-902
EN
The aim of this study was to assess the course of some parameters of non-specific immunity during the estrus cycle in bitches. Blood samples were collected from 28 healthy bitches at different stages (anestrus, proestrus, estrus, metestrus) of the estrus cycle. Nitroblue tetrazolium (NBT) reduction by blood neutrophils, phagocytic activity of neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin, level of total protein and γ-globulin fraction were determined. The study showed that nitroblue tetrazolium (NBT) reduction by blood neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin were significantly higher (p ≤ 0.01) in proestrus and estrus than in anestrus.
EN
The aim of this analysis was a comparison of the incidence of reproduction disorders, clinical mastitis and lameness in Holstein-Friesian and Jersey cows maintained at the same farm during three years. The herd consisted of 125 cross-breed HF × BW cows and 39 Jersey cows. Incidence of silent heat was 28.5% in HF, and 26.3% in Jersey. Cystic ovarian disease occurred in 3.8% HF and 3.9% of Jersey cows. Retained placenta was observed in 6.6% HF and 3.1% Jersey, and endometritis in 8.9% and 4.4%, respectively. 13.5% HF suffered from lameness and only 1.5% Jersey (p £ 0.05). Prevalence of clinical mastitis was at a similar level (HF 54.7%, Jersey 50.7%). Incidence of reproduction disorders and clinical mastitis was very similar for both analyzed breeds, despite of the difference of milk production. On the other hand there was considerably less lameness in Jersey than in Holstein cows.
EN
This paper presents current information on the regulatory mechanism of the endocrine function of the corpus luteum in cyclic and pregnant dogs. Corpus luteum function in the first half of diestrus or pregnancy (< day 30) is independent of gonadotrophins. The mechanisms regulating corpus luteum function in this phase are largely unknown. It seems that an important role in the controlling of progesterone biosynthesis is played by StAR (steroidogenic acute regulatory protein) and 3β-HSD (3β-hydroxysteroid dehydrogenase). Recently, it has been demonstrated that prostaglandin E2 acts luteotrophically by increasing the expression of StAR. In addition, the action of progesterone on its receptors at the para-/autocrine level appears to serve as a luteotrophic factor. There is no significant difference in the regulation of corpus luteum function between pregnant and non-pregnant bitches during this time. Corpus luteum function is fully gonadotrophin-dependent during the second half of diestrus. Prolactin and, to a lesser extent, LH are the main luteotrophic factors. The slow process of luteal regression starts by day 30 after ovulation, and it takes place in spite of the increased availability of pituitary luteotrophic hormones (LH, prolactin). During luteal regression, progesterone concentration gradually decreases. This decrease is caused by a reduced expression of StAR and 3β-HSD, as well as by degenerative changes in the luteal cells. In nonpregnant bitches, progesterone concentration decreases slowly and reaches baseline values 80-90 days after ovulation. In pregnant bitches, on the other hand, progesterone decreases rapidly 1-2 days before parturition. In non-pregnant bitches, luteal regression is a passive process in the absence of luteolytic factors, whereas in pregnant bitches, luteolysis is an active process. This is due to a rapid progesterone decrease to the threshold level and the release of PGF2α from the fetal part of the placenta.
EN
The aim of the study was to determine the effect of lactation on blood biochemical components and body weight in Kamieniec ewes nursing singles or twins. The examinations were conducted on 4-year-old ewes; 12 suckling single lambs and 12 suckling twin lambs. The feeding level was maintained constant during a 70-d lactation period. The course and consequences of lactation in ewes nursing singles and twins were compared taking into account daily milk yield, milk production over lactation, milk chemical composition, and blood biochemical indices. Milk yield was found to be significantly (by more than 20%) higher in ewes nursing twins, reaching 27.40% towards the end of lactation. Milk production over lactation was higher in the mothers of twins, by 23.67 kg (P≤0.05). No significant differences were observed between ewes suckling singles and twins with respect to the chemical composition of milk. Changes in blood biochemical indices, primarily in the activity of ALT and AST, concentrations of protein, urea, and creatinine, as well as in the parameters of acid-base balance indicate that lactation has a greater challenge in the ewes nursing twins.
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tom 45
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nr 05
RU
В 4 коровниках с разным кормлением и окружающей средой секрецию прогестерона в межбеременный приод определили на основе уровня этого гормона в крови либо молоке. Репродукцию коров в исследуемых стадах оценили при помощи клинических исследований, а также длины межбеременного периода и величины показателя беременности. В коровнике с наиболее благоприятными условями кормления и зоогигиены 74% коров показывало физиологическую секрецию гормона, тогда как в остальных коровниках этот процент был ниже и составлял 37—53%. В исследуемых стадах зависимость между условиями кормления и зоогигиены а выделением прогестерона не была однозначной. Отметили, что условия содержания влияют на длину послеродовой ациклы, вид же секреции прогестерона, кажется, менып от них зависит. Кроме того показали, что хорошая плодовитость коров связана, главным образом, с быстрым началом лютеальной функции яичников после родов, в меньшей же степени с характером секреции прогестерона. Отметили притом, что в многих случаях субклинические нарушения выделения гормонов не понижали плодовитости животных. Нарушения инволюции матки и воспалительные состояния слизистой оболочки матки были, как правило, связаны с неправильной секрецией прогестерона.
EN
The level of progesterone in the blood and milk was determined in cows haused under different environmental conditions and being on different diet. The reproduction of cows was assessed clinically and on the basis of the lenght of the period between pregnancies and the value of pregnancy index. In a cowshed with most favourable nourishment and zoohygienic conditions 74% of animals produced that hormone normally, while in the rest of cowsheds these indices were lower and were 37—53%. The interrelationship between nourishment and zoohygienic conditions and the production of progesterone in the herds under study was not univocal. It was found that the conditions of maintenance influenced the length of post-partem acycles; however, the kind of progesterone production seemed to be less essential. Besids, it was found that good fertility of cows was related chiefly with the quick start of luteal production by ovaries after parturition. In many cases subclinical disturbances in the secretion of the hormone did not influence the fertility of animals. However, the disturbances regarding uterus involution and metritis were usually related with unphysiological progesterone production.
EN
Semen quality parameters of infertile and fertile dogs were compared. Sperm concentration and semen motility parameters were measured by the Hamilton-Thorne Semen Analyser IVOS 12.3. The spermatozoal morphology and the percentage of live spermatozoa were examined microscopically. Forty-six dogs of various breeds were examined. Twenty dogs had a conception failure within last year. These dogs had a history of prior normal fertility. Twenty six fertile dogs served as control. All animals underwent a clinical examination as well as ultrasonography. Sperm concentration was significantly lower in infertile dogs than in fertile dogs. For most determined motility parameters (MOT, PMOT, VAP, VSL, VCL, BCF, RAPID, STATIC) significant differences between infertile and fertile dogs were found. The percentage of spermatozoa with normal morphology also significantly differed between both groups. Ultrasonography of the prostate gland and testes revealed no pathological conditions. The testicular degeneration was assumed to be a possible cause of infertility in these dogs. The present study showed that the most sperm CASA motility parameters were significantly lower in infertile dogs in comparison to the fertile ones, and confirmed the usefulness of the Hamilton-Thorne Semen Analyser for a quick and objective analysis of sperm concentration and motility in dogs.
EN
Currently, subclinical endometritis is being discussed as an important cause of reduced conception rates in dairy cows. Inflammation of the endometrium alters the uterine environment and disrupts conception or embryo survival. Subclinical endometritis mainly results from persistent bacterial infection during parturition. Arcanobacterium pyogenes, Prevotella spp., Fusobacterium necrophorum and Escherichia coli are major uterine pathogens. The association between infection with bovine herpes virus 4 and endometritis is suggested. Subclinical endometritis has been histologically diagnosed in 30-50% of repeat breeding cows. Recently, uterine cytology collected by cytobrush or lavage is used for the diagnosis of endometrial inflammation. Subclinical endometritis is defined as the presence of > 18% neutrophils in uterine cytology collected 21-33 days postpartum or > 10% neutrophils at 34-47 days. Prevalence of subclinical endometritis in different studies ranges from 30 to 70 percent. There are no practical recommendations for the treatment of subclinical endometritis. The use of prostaglandin F₂a and intrauterine infusion of antibiotics or proteolytic enzymes has been described.
EN
Granulosa cells were isolated from large (≥8 mm) follicles and incubated for 24 h without or with LH (100 ng/mL), daidzein, genistein or equol in doses of 0.5, 5, 10, and 50 µg/mL each or combination (3:13:39) of these isoflavones at a dose 50 µg/ml. The concentration of oestradiol-17ß in incubation media was measured by RIA. All the isoflavones at used doses decreased significantly basal and LH-stimulated secretion of oestradiol-17ß by granulosa cells in a dose dependent manner. The combination of these isoflavones also inhibited significantly oestradiol-17ß secretion by the cells. These data suggested that decreased secretion of oestradiol-17ß caused by dietary isoflavones could be a reason of silent heat in cows.
EN
The study was conducted on 4-year-old Kamieniec ewes, 12 suckling single lambs and 12 suckling twin lambs. The course and consequences of lactation in ewes nursing singles and twins were compared based on daily milk yield, blood hematological indices, blood mineral concentrations and wool quality traits. Milk yield was found to be significantly higher in ewes nursing twins - by 21.46% at the peak of lactation, and by as much as 27.40% towards the end of lactation. Blood hematological indices were not affected by the number of suckling lambs. A significant decrease in Ca concentration observed in all ewes at peak milk yield was followed by an increase. There was a tendency towards a gradual decrease in Mg levels during lactation, but significant differences were reported only with respect to ewes nursing twins. The process of uterine involution at first proceeded faster in ewes suckling twins, but starting from day 30 p.p. no differences were noted between the groups. Lactation had a negative influence on wool quality in both groups, but the decrease in wool growth rate as well as in staple thickness and strength was much more significant in ewes nursing twins.
EN
Concentrations of oestrogens in the blood plasma and seminal plasma of mature boars are high. However, little is known about their concentrations after reaching sexual maturity. The aim of this study was to determine the concentration of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period. Free and conjugated oestrone and oestradiol-17β were determined by radioimmunoassay in blood from the testicular vein and artery, and peripheral circulation as well as in seminal plasma collected from 18 Polish Landrace boars. The animals were divided into three groups (n = 6) according to age (8, 12 and 16 months, respectively). Oestrone was predominant free and conjugated oestrogen. The highest values of oestrogens were measured in the testicular vein (p ≤ 0.05). The concentrations of oestrogens in seminal plasma did not differ from those found in the peripheral circulation. An age-dependent increase in levels of all four oestrogens (p ≤ 0.05) was observed. This can be associated with biochemical maturation of the reproductive system during the postpuberal period.
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