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Primordial germ cells ( PGCs) as a tool for creating transgenic chickens

100%

Chojnacka-Puchta L. , Kasperczyk K. , Plucienniczak G. , Sawicka D. , Bednarczyk M.

Polish Journal of Veterinary Sciences

2012

tom 15

nr 1

The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.

Obtaining chicken primordial germ cells used for gene transfer: in vitro and in vivo results

86%

Chojnacka-Puchta L. , Sawicka D. , Lakota P. , Plucienniczak G. , Bednarczyk M. , Plucienniczak A.

Journal of Applied Genetics

2015

tom 56

nr 4

Flow cytometric analysis of apoptosis in cryoconserved chicken primordial germ cells

86%

Sawicka D. , Chojnacka-Puchta L. , Zielinski M. , Plucienniczak G. , Plucienniczak A. , Bednarczyk M.

nr 1

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

86%

Chojnacka-Puchta L. , Kasperczyk K. , Płucienniczak G. , Sawicka D. , Bednarczyk M.

nr 1