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2 Biotechnologia

2 1993

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Pilot plant and industrial systems for animal cell cultivation. II. Anchorage independent cell cultures

100%

Grajek W.

1993

nr 2

22-34

In the last decade fast development of modern cell culture techniques is observed. Some new high performance cell culture bioreactors are being designed and tested. These reactors are usually configurated with cell separators to cell recycling. As separators, microfilters, ultrafilters, continuous flow centrifuges and settlers are proposed. The main systems allowing to carry out the high cell density cultures and numerous technological applications of these systems are presented in this review.

Pilot plant and industrial systems for animal cell cultivation.I.Anchorage-dependent cell cultures

63%

Grajek W. , Lukaszynski D.

1993

nr 2

8-21

Several methods used to cultivate anchorage-dependent animal cells on a large scale are presented. These methods include the cultures on microcarriers in stirred and air-lift bioreactors, the cultures in hollow-fiber and flat-plate membrane bioreactors as well as the cultures attached to porous or fiber solid supports. The critical parameter in anchorage-dependent cultures is the adhesiveness of the cells to the carrier materials and the surface proliferation rate.