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Determination of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses by duplex PCR, immunohistochemistry and immunofluorescence methods

100%

Ozkaraca M. , Irehan B. , Parmaksiz A. , Ekinci A. I. , Comakli S.

Medycyna Weterynaryjna

2017

tom 73

nr 06

This study was aimed at determining the existence of Neospora caninum and Toxoplasma gondii in aborted bovine foetuses. In this research, 102 bovine foetuses were examined by duplex PCR, immunohistochemistry and immunofluorescence to determine the presence of N. caninum and T. gondii. None of the aborted bovine foetuses were shown to have T. gondii, but N. caninum was detected in 26 foetuses (25.49%) by duplex PCR, in 18 (17.64%) by immunohistochemistry and in 8 (7.84%) by immunofluorescence. Moreover, 16 livers, 13 kidneys, 12 spleens, 8 thymuses and 5 brains of the 18 foetuses examined by immunohistochemistry showed immunopositivity. Positive staining was found in the spleen of eight foetuses by the immunofluorescence method. In this study, immunohistochemistry was shown to be superior to immunofluorescence in terms of diagnosis. Although immunofluorescence has the advantage of being the easiest to use in practice, it makes diagnosis more difficult because of its non-specific staining.

Activity of resveratrol on the influence of aflatoxin B1 on the testes of Sprague dawley rats

100%

Omur A. D. , Yildirim B. , Saglam Y. S. , Comakli S. , Ozkaraca M.

Polish Journal of Veterinary Sciences

2019

tom 22

nr 2

Arginase activity and total oxidant/antioxidant capacity in cows with lung cystic echinococcosis

86%

Hanedan B. , Kirbas A. , Kandemir F. M. , Ozkaraca M. , Kilic K. , Benzer F.

Medycyna Weterynaryjna

2015

tom 71

nr 03

The aim of this study was to investigate arginase activity, total oxidant capacity (TOC), total antioxidant capacity (TAC), and oxidative stress index (OSI) in cows with lung cystic echinococcosis (CE). The subjects were 20 cows with lung CE (parasitized group) and 20 healthy cows (control group). A significant increase in arginase activity, TOC, and OSI, and a significant decrease in TAC were found in the parasitized group compared with the control group (P < 0.001). Histopathological examination revealed a fibrous tissue reaction with inflammatory cell infiltration surrounding the CE in the lungs. The study revealed that increased arginase activity, TOC, and OSI levels and decreased TAC levels occurred in lung CE in cows. Increased arginase activity may be an important parameter for pulmonary fibrosis associated with lung CE in cows. Oxidant production due to chronic inflammation, as evidenced by the increased OSI levels, might contribute to persistent lung injury

Effect of intrauterine infusion of Momordica charantia L. on oxidative stress and pregnancy rate in infertile cows

86%

Emre B. , Korkmaz O. , Temamogullari F. , Zonturlu A. K. , Koyuncu I. , Ozkaraca M. , Cengiz M.

Journal of Veterinary Research

2017

tom 61

nr 4