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tom 62
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nr 12
1395-1399
EN
The purpose of the field trials was to establish the efficacy of the acute clinical mastitis treatment with antibiotics in connection with an i.v. injection of an immunomodulator (lysozyme dimer). The trials were conducted on 164 cows (190 sick quarters) divided into 4 groups (8 subgroups) after diagnosis was carried out and milk samples were taken. Four antibiotic products (containing: 1/ Amoxycillin + Clavulanic acid + Prednisolone, 2/ Lincomycin + Neomycin + Prednisolone, 3/ Neomycin + Penicillin G + Novobiocin + Streptomycin + Prednisolone, 4/ Penicillin + Neomycin) were given intramammary infusions at doses recommended by manufacturers with or without (control subgroups) one i.v. injection of lysozyme dimer at a dose of 10 µg/kg b.w. (experimental subgroups). Examinations checking results (clinical, bacteriological, SCC) were conducted on the 7th, 14th, and 21st days after treatment. The criteria of recovery were: regression of clinical signs in the udder, normal appearance of milk, decline of bacteria in milk, decrease of SCC. The recovery rates were 74.1% (1), 53.8% (2), 43.5% (3) and 50.0% of the quarters (4) in control subgroups versus 80.0%, 56.0%, 65.2% and 57.1% of quarters, respectively, in experimental subgroups. An average recovery of 56.3% of the quarters treated with intramammary infusions of antibiotic products and 64.9% of the quarters treated with the same antibiotics connected with the single injection of lysozyme dimer (p = 0.11). The average recovery rate among control cows was 56.1% vs. 65.8% among experimental cows (p = 0.1). The injection of the immunomodulator resulted in an increase of recoveries in acute mastitis cases caused by Str. uberis (from 28.3% to 76.9%), CNS (from 68.7% to 90.9%), E. coli (from 16.7% to 63.3%, and A. pyogenes (from 0% to 16.7%) or Staph. aureus (from 0% to 40%). In conclusion, the intramammary antibiotic products at the recommended doses are efficient enough in clinical mastitis cases caused by Str. agalactiae and CNS, poorly efficient in cases caused by E. coli and Str. uberis and not at all in cases caused by Staph. aureus and A. pyogenes. One i.v. injection of lysozyme dimer increases the efficacy of intramammary antibiotic treatment of acute forms of mastitis caused by CNS, Str. dyagalactiae, Str. uberis, E. coli, and even Staph. aureus or Arcanobacterium pyogenes.
EN
The purpose of the study was to evaluate the metabolic activity of neutrophils in pure blood healthy and mastitic cows. Examinations were performed on 10 cows with clinical mastitis, 10 with subclinical mastitis and 10 healthy cows. The blood samples were collected from the coccygeal vessels and milk veins on the first day of clinical mastitis treatment with an intramammary product containing amoxicillin, clavulanic acid and prednisolone (or observation of the other cows) and on 3rd and 7th days. The blood was examined in the following ways: without in vitro stimulation and in vitro stimulation using fMLP, OZ, PMA. The reactive oxygen species (ROS) production was assessed by means of luminol-enhanced chemiluminescence (CL) using a kinetic method for 40 minutes at 38°C, measuring CL at 5 minute intervals (BioOrbit 1251 Luminometer). The area under the curve (integrate) was calculated. Significant differences in CL levels between peripheral blood and blood flowing from the udder were not found, irregardless of the use of stimulators or udder health status. The spontaneous and induced CL level, mainly through means of the receptor, was significantly higher in the blood of mastitic cows in comparison to healthy cows. PMNs from chronic mastitic cows were characterized by weakness of oxygen metabolism and a crucial increase of a stimulated respiratory burst through activation of the protein kinase C way (PMA). A significant decrease of OZ stimulated and non significant decrease of PMA stimulated CL was determined on the 3rd and 7th days after intramammary clinical mastitis treatment; however, the spontaneous CL remained on the same level.
EN
This research paper addresses the hypothesis that the incidence of various bovine mastitis pathogens is subject to change over time and across regions. The aim of the research was to determine the pathogen species that are currently the etiological agents of mastitis in Polish dairy herds. This comprehensive study was conducted in the period 2009-2017 and included all the Polish provinces, distinguishing between those with high and low numbers of dairy cattle. Over eight years 38,852 quarter milk samples were obtained from 22,587 cows with clinical and subclinical mastitis from herds located in all 16 provinces in Poland. Milk samples were cultured using standard microbiological methods. Most of them were positive for microorganisms but there was also part of bacteriologically negative and contaminated samples. Gram-positive catalase-negative cocci and staphylococci were the most prevalent pathogens. The growth of other microorganisms such as Gram-negative and Gram-positive bacilli, yeasts and algae was observed at lower frequencies. There were differences in the prevalence of microorganisms depending on the region. In the eastern part of the country a higher number of Streptococcus agalactiae infections than in other regions was observed. This paper provides the latest data on microbial species, frequency of their occurrence and regional location within Poland. The knowledge of mastitis pathogens enables appropriate treatment and to make prevention recommendations for the herds.
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