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3
51%
EN
287 sera were examined by means of the complement fixation reaction, applying the cold and warm method. 282 were human and 5 rabbit sera. The results of these examinations may be summarized as follows: 1) Positive results were obtained: by the warm method - 5,92%, by the cold method - 14,79%. 2) The titre with the cold method is always higher than the titre obtained with the warm method. 3) The conformity of the results of the complement fixation, Sabin-Feldman's dye test and Frenkel's intradermal test amounts to 61.5% for the warm method and to 66.6% for the cold method. The conformity of the ,results of the complement fixation and Frenkel's intradermal test amounts to 54.8% for the warm and 58.06% for the cold method. The conformity of the results of the complement fixation test and the dye test amounts to 84% for both methods.
EN
Shepherd dogs (276), males, at the age of ca. 1 year, from various regions of Poland, were examined. The complement fixation test with the antigen obtained from the exudate of infected mice and intracutaneous test (0.1 ml of toxoplasmine 1:100-1:1000, reading after 24-28 hours, control test with spleen exudate from healthy mice) were performed paralelly. A positive result of both tests was obtained in 10 dogs (4%): in 52 dogs (19%) it was found to occur only with the romplement fixation (titre 1:3-1:8) and in 30 ones (11%) only with the intracutaneous test.
EN
The aim of this work was to determine the role of toxoplasmosis in the pathology of pregnancy among women in the Lublin province, and simultaneously the attempt to evaluate the correlation between three diagnostic methods: Frenkel's intradermal test, Sabin-Feldman's dye test and complement fixation reaction. 396 women were examined and 332 simultaneously submitted to serological and allergical investigations. In 64 cases the investigations were only carried out with the complement fixation and intradermal tests. The highest number of positive results was obtained by the intradernal reaction, i. e. 33.8%, the lowest by the complement fixation reaction 8.3%. The dye test gave 10.6%. The conformity of the results for all three tests amounts to 56.7% (225 cases). In the remaining cases (171) the conformity of results between the individual tests was studied, and so: for the complement fixation and the dye tests it amounted to 15.6%, for complement fixation and Frenkel's intradermal tests to 12.5%, for the dye test and Frenkel's intradermal test - 6.7%. The remaining 9,5% fell on the unconformity between the results of the complement fixation reaction and Frenkel's intradermal test. The highest percentage of infections has been observed in the group of premature deliveries; that is 56.6%. To this group belongs also a relatively great number of stillborn children and children with development defects. In the group of women who suffered abortion the percentage of positive results is the lowest – 21.05%. The percentage of positive results is almost identical in the goup of women who were not suspected of toxoplasmosis and in the group where this suspicion existed (32.1%-34,7%). An exact anamnesis concerning the pregnancies of 241 women (multiparae) has shown that the number of abortions in the group of women with positive results is by 50% higher than in the group with negative results. The number of nonviable fetuses is by 2.9 times higher and the number of premature deliveries by 30% higher. The number of infants with development defects is 3 times higher in the group of women who were infected with toxoplasmosis. The number, however, of stillborn children with positive results amounts to 3,8%, while in the group of women with negative results to 5.6%. In cases of habitual abortion, premature delivery or development defects the possibility of toxoplasma infection in the mother should be taken into consideration and laboratorial investigations carried out, such as: complement fixation reaction, Sabin-Feldman's dye test and Ftenkel's intradermal test. A positive results of even one of the serological test in comparison with clinical observations makes an anti-toxoplasmosis treatment unavoidable.
EN
The authors compared the reaction in 114 women caused by toxoplasmine produced by the Parasitological Department of the "Universita Karlova'' in Prague and that one produced by the Parasitological Department of the Institute of Labour Medicine and Rural Hygiene in Lublin. At an equal dilution of antigens (1 : 1000) the first toxoplasmine induces more frequent and violent skin reactions. Some differences, which could not be nearer explained, were also observed when applying various dilutions. An exact standardization of the antigens aind diagnostic methods for toxoplasmosis should be introduced in order to be able to compare different results.
EN
The aim of the study was determination of selected parameters of immunological response among hop growers and farmers in conditions of intensive exposure to means of plant protection. Survey data was collected from 238 males aged 25-70 living in the area of Wilkow near Pulawy (Lublin Region). Control group were males from the area of Witoszyn (Lublin Region) - 53 people aged 25-70 occupied mainly with land cultivation. Based on an environmental survey conducted among hop growers and farmers, the respondents were divided into 3 age groups: 25-40, 41-55 and 56-70. Laboratory tests covered the determination of selected morphological parameters, phagocytic test, NBT test, and myeloperoxidasis (MPO) concentration in blood serum of hop growers and farmers.A significant decrease was noted in the number of platelets in the general population of hop growers and in individual age groups, compared to the control groups of farmers. Analysis of individual sub-populations of leukocytes showed a significantly higher number statistically of basophils and lymphocytes among hop growers, compared to farmers. A detailed analysis of the degree of phagocytic and bactericidal activity of neutrophils allowed us to presume that during the period of spraying there occured a mobilisation of the granulocytic system, manifested by the presence of over 90% of neutrophils of intensified phagocytic activity, and 20% of neutrophils of intensified bactericidal activity. The preparations prepared by the routine NBT test method were analysed with the use of LUCIA computer programme (version 4.51). The analysis of the level of MPO in blood serum in the populations examined showed the presence of statistically significant differences. In hop growers, the MPO level was significantly higher statistically (60.0 ng/ml), compared to the control group of farmers (43.4 ng/ml).
EN
Two organic compounds of selenium, 4-o-totyl-selenosemicarbazide p-chlorobenzoic acid (chain compound) produced at the Chemistry Department of the University Medical School in Lublin, and one inorganic compound of sodium IV selenite (Na2SeO3) were used. The preparations were used per os in doses of 1 mg/kg body weight and 0.5 mg/kg body weight. The studies were conducted on female Swiss mice, covering seven groups of animals, i.e. 6 experimental and 1 control. Histopathologic changes were observed in liver, kidney, lung and heart. Ultrastructural changes were observed in liver and kidney. Our studies indicate a dose-dependent effect of selenium on histopathologic and ultrastructural changes. It is possible therefore, that the extent of excess of selenium exerts a greater influence on a cell than the form of supplemented selenium.
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