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1 Journal of Physiology and Pharmacology

1 1991

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Does the membrane potential control incorporation of tubulovesicles into the secreting apical membrane of the rat parietal cell?

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Ostrowski J. , Dolowy K. , Zych W. , Butruk E.

Journal of Physiology and Pharmacology

1991

tom 42

nr 4

The present studies were designed to examine the effect of changes in membrane potential by means of protonophore carbonyl cyanide m-chlorophenylhydrazone (СССР) and variations in the pH of the medium on the secretory response of parietal cells. Studies were performed in vitro using isolated cells from rat stomachs and acid production was indirectly determined by ¹⁴C-aminopyrine (AP) accumulation. СССР affected both basal and histamine-stimulated AP accumulation in a concentration-dependent manner. The AP accumulation ratios depended on pH of the incubation medium; the ratio was lowest at pH 6.6, and increased progressively as the pH of the medium increased to 7.8. Moreover, the decreases in AP accumulation ratios caused by simultanous addition of СССР and AP to cell suspensions compared to those in which СССР was added to incubated cells after achieving the steady-state of AP accumulation were quantitatively similar. These findings suggest that the decrease in AP accumulation due to СССР treaiment is a consequence of an activation of acid secretion rather than an inhibitor of acid production. From the present and previously published data, we propose a working hypothesis: membrane recycling is dependent on changes in apical membrane potential.