Wyniki wyszukiwania - Biblioteka Nauki

1

Regulacja funkcji sekrecyjnej komórek jajnika świń w czasie cyklu estralnego, ciąży i ciąży rzekomej

100%

Przala J.

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tom 339

2

The effect of oxytocin [OT] and VIP on the PRL and LH secretion by porcine pituitary cells after treatment with exogenous progesterone

63%

Bogacka I. , Przala J.

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1997

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tom 35

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nr 2

3

The involvement of catecholamines in the pituitary release of luteinizing hormone [LH] and prolactin [PRL] in progesterone [P4] - treated gilts

63%

Siawrys G. , Przala J.

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1997

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tom 35

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nr 2

4

Wpływ hormonu luteinizującego (LH), prolaktyny (PRL) i hCG na sekrecję estradiolu 17 beta przez komórki lutealne świń z wczesnego okresu ciąży

51%

Przala J. , Wiesak T. , Grazul A.

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tom 309

5

Influence of PGF2a on progesterone synthesis by porcine corpus luteum in vitro

51%

Przala J. , Krzymowski T. , Grazul A.

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tom 30

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nr 1

6

Ontogeny of the long form of leptin receptor gene expression in the porcine ovarian follicles

51%

Smolinska N. , Kaminski T. , Siawrys G. , Przala J.

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nr 1

7

Ontogeny of the long form of leptin receptor gene expression in the porcine ovarian follicles

51%

Smolinska N. , Kaminski T. , Siawrys G. , Przala J.

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2013

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tom 16

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nr 1

EN

Leptin is a polypeptide hormone produced predominantly in adipocytes. It has been found to be implicated in the regulation of satiety and energy homeostasis. A role for leptin in reproduction was later suggested by findings that this hormone may be involved in the regulation of the hypothalamic- pituitary-gonadal axis via endocrine, paracrine and/or autocrine pathways. The objective of the study was to investigate the ontogeny of the long isoform of leptin receptor (OB-Rb) gene in porcine ovarian follicles. The expression of OB-Rb gene was detected in porcine primordial, primary, secondary and antral follicles by in situ hybridization. In summary, our data suggest that leptin might have a direct effect on porcine follicles and plays an important role in the follicular development.

8

Wpływ oksytocyny na komórki lutealne świń z wczesnego okresu ciąży

51%

Przala J. , Grazul A. , Wiesak T.

|

tom 309

9

Profilaktyczne zastosowanie preparatu Biolactin-2 u loszek remontowych a syndrom MMA

51%

Przala J. , Gajecki M. , Przala F. , Ryszka F.

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nr 01

31-33

EN

The effect of Biolactin-2 preparation, containing 5 mg of purified prolactin, on the occurrence of MMA syndrome and the number and weight of piglets in the litter was studied. The study was carried out on 212 gilts of which 150 were prophylactically administered Biolactin-2 preparation. Clinical examinations showed that MMA syndrome occured only in 7,33% of gilts in the group receiving Biolactin-2, while in the control group, it occured in 41,9%. Moreover, a higher body weight of the litter of 5.24 kg and larger litter size of 1.7 piglet, was ascertained at day 10 after birth amoung those given the preparation.

10

Wpływ estradiolu 17 beta (E2) i hormonu luteinizującego LH na sekrecję progesteronu (P4) przez komórki lutealne pochodzące od loch w ciąży i w ciąży rzekomej in vitro

51%

Grazul A. , Przala J. , Wiesak T.

|

tom 309

11

Expression of orexin receptors 1 [OX1R] and 2 [OX2R] in the porcine hypothalamus during the oestrous cycle

51%

Kaminski T. , Smolinska N. , Nitkiewicz A. , Przala J.

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tom 61

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nr 3

363-371

EN

Orexin A and B are hypothalamic peptides involved in the control of food intake, sleep patterns, autonomic and neuroendocrine systems. The biological actions of orexins are mediated via two G-protein coupled receptors, termed orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). The present study analyzed mRNA and protein expressions of OX1R and OX2R in porcine hypothalamic structures engaged in GnRH production and secretion, preoptic area (POA), mediobasal hypothalamus (MBH), and stalk median eminence (SME) on days 2-3, 10-12, 14-16, and 17-19 of the oestrous cycle. The highest OX1R gene expression in POA was observed on days 17-19 of the cycle. Changes in the mRNA expression in MBH and SME throughout the cycle were negligible. The expression peak of OX2R gene in POA and SME occurred on days 17-19 as well. There were no changes in the gene expression in MBH. OX1R protein content in POA and SME also was the greatest on days 17-19 and OX2R protein expression was most pronounced in MBH and SME during the same phase of the cycle. In conclusion, fluctuation of OX1R and OX2R mRNAs and proteins content in pig hypothalamus dependently on the phase of the oestrous cycle suggests that orexins, through the influence on the hypothalamic-pituitary-ovarian axis, may affect reproductive functions.

12

Leptin gene and protein expression in the trophoblast and uterine tissues during early pregnancy and the oestrous cycle of pigs

51%

Smolinska N. , Siawrys G. , Kaminski T. , Przala J.

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nr 3

EN

Leptin is a 16-kDa protein hormone encoded by the obese (ob) gene and acts on receptors in the hypothalamus to regulate food intake and energy balance. The identification of leptin and its receptor mRNAs and proteins in human and mouse endometrium and placental trophoblast has attracted attention to the potential role of leptin in implantation. Thus, the aim of this study was to compare the expression levels of porcine leptin mRNA and protein in endometrium and myometrium during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of pregnancy respondent to the beginning (days 14 - 16) and the end (days 30 - 32) of the implantation process, and in trophoblast during both periods of pregnancy. Leptin gene and protein expression in myometrium, and leptin mRNA expression in endometrium was more pronounced in the mid- and late-luteal phases of the cycle in comparison to studied periods of pregnancy, whereas leptin protein concentration in endometrium was either enhanced on days 30 - 32 of pregnancy in relation to days 14 - 16 of the cycle or there were no changes between pregnancy and luteal phase of the cycle. On days 30 - 32 of pregnancy, expression of the leptin gene in the endometrium, and of the leptin gene and protein in the myometrium was more pronounced in comparison to the earlier stage of pregnancy. Moreover, leptin gene expression in porcine trophoblast increased during the beginning of the implantation process compared to days 30 - 32 of pregnancy, while the protein concentration decreased on days 14 - 16 of pregnancy. In conclusion, the finding of leptin gene and protein expression in porcine endometrium, myometrium and trophoblast indicates that locally synthesised leptin can participate in the control of pig reproduction. The fluctuation of the hormone concentration during pregnancy and changes in its level between pregnancy and the oestrous cycle may indicate leptin's involvement in the implantation process.

13

Udzial opioidow w lokalnej regulacji funkcji jajnika

45%

Przala J. , Kaminski T. , Okrasa S. , Siawrys G. , Bogacka I.

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1999

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tom 12

113-116

14

Ocena w warunkach in vitro luteolitycznych właściwości pochodnych prostaglandyny F2alfa zsyntetyzowanych w Polsce

38%

Grazul-Bilska A. , Przala J. , Wiesak T. , Arendarcik J. , Stanikova A. , Muszynska A. , Koziorowski M.

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nr 02

RU

Цель настоящей работы состояла в оценке лютеолитических свойств 3 польских производных простагандина F₂ α (PGF): Cloprostenol PW₁, PW₂ и сравнении их лютеолитическими свойствами Oestrophan (Spofa, Чехословакия) и Enzaprost (Chinoin, Венгрия) с использованием лютеальной ткани скота, содержащейся в перфузионной системе in vitro. Срезы этой ткани помещали в 6 камерах, входящих в cocтав nepфузионнои cистемы, череѕ которую протекала питательная среда со скоростью 02,5 мг/мин. Среду собирали каждые 10 мин в течение 2 часов. Пo исгечении иeрвых 50 мин. протекания среды, не содержащей экзогенных гормонов, использовали среду, содержащую или нет разные виды простагландинов в концентрации 1 µг/мл: Cloprostenol, PW₁, PW₂, Oestrophan, Enzaprost. В течение последних 40 мин. опыта ткань перфундироьали сызнова средои, не содержащей просгагландинов. Пpooы хранили в темн. -18℃ перед определенем в них содержания прогосмерона (P₄) радиoиммуыолотическим, методом. Показали, что все примененые простагандины тормозили (Р<0,01) освобождение Р₄ лютеальной тканью скота. Польские производные PGF показываюот похожие лютеалитические свойства как препараты Oestrophan и Enzaprost. Все 3 польские производные PGF можно рекомендовать для применения в ветеринарно-зоотехнической практике.

EN

Luteolytic activity of three Polish prostaglandin F₂ α derivatives (PGF): Cloprostenol PW₁ and PW₂ in comparison to Oestrophan (Spofa, Czechoslovakia) and Enzaprost (Chinoin, Hungary) was investigated using a bovine luteal tissue in the in vitro perfusion system. The slices of luteal tissue were placed into 6 chambers with a medium flow rate 0.25 ml/min in 37°C. Medium was collected at 10 min. intervals for 2 hours. After the first 50 min. of tissue perfusion without exogenous prostaglandins for the next 30 min. was used medium with or without Cloprostenol, PW₁ and PW₂, Oestrophan or Enzaprost at a concentration 1 µg/ml. By the last 40 min. tissues were perfused again with media without exogenous hormones. The samples were stored at -18°C until the determination of the content of progesterone (P₄) by radioimmunoassay. It was found that all used prostaglandins inhibited (P<0.04) P₄ secretion by a bovine luteal tissue. Polish PGF derivatives show a similar luteolytic activity as Oestrophan and Enzaprost. All three Polish PGF derivatives may be recommended in veterinary and husbandry practice.