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L' offre et la demande des e-competences des employes en Pologne - une approche d'estimation

100%

Borucki W. , Springer A.

Studia Ekonomiczne

2013

tom 150

203-217

Parler des compétences des travailleurs conduit à évoquer les besoins, lacunes ou incompétences qui proviennent de l'utilisation des technologies de communication électronique. Paralèllement, les changements sur le marché du travail polonais indiquent que le travail mental non routinier prend une place de plus en plus importante. Compte tenu de l'importance et de l'impact des /incompétences /dans le travail effectué dans différents groupes professionnels, on peut déjà observer une lacune évidente, à la fois de nature cognitive et sociale.

Alteration of O-specific polysaccharide structure of symbiotically defective Mesorhizobium loti mutant 2213.1 derived from strain NZP2213

80%

Turska-Szewczuk A. , Pietras H. , Borucki W. , Russa R.

Acta Biochimica Polonica

2008

tom 55

nr 1

191-200

Mesorhizobium loti mutant 2213.1 derived from the wild-type strain NZP2213 by Tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant Lotus corniculatus (Turska-Szewczuk et al., 2007 Microbiol Res, in press). The inability of lipopolysaccharide (LPS) isolated from the mutant 2213.1 strain or de-O-acetylated LPS of the parental cells to inactivate phage A1 particles implicated alterations in the LPS structure. The O-specific polysaccharide of the mutant was studied by chemical analyses along with 1H and 13C NMR spectroscopy, which clearly confirmed alterations in the O-chain structure. 2D NMR data showed that the mutant O-polysaccharide consists of a tetrasaccharide repeating unit containing non-substituted as well as O-acetylated or O-methylated 6-deoxytalopyranose residues. Additionally, an immunogold assay revealed a reduced number of gold particles on the mutant bacteroid cell surface, which could result from both a diminished amount of an O-antigenic determinant in mutant LPS and modifications of structural epitopes caused by alterations in O-acetylation or O-methylation of sugar residues. Western immunoblot assay of alkaline de-O-acetylated lipophilic M. loti NZP2213 LPS showed no reactivity with homologous serum indicating a role of O-acetyl groups in its O-specificity.