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Cloning of cDNA of the cysteine proteinase gene from Ancylostoma ceylanicum - a potential vaccine antigen

100%

Mieszczanek J. , Wedrychowicz H.

Wiadomości Parazytologiczne

1998

tom 44

nr 3

Differentiation of two dog hookworm species using PCR

100%

Mieszczanek J. , Wedrychowicz H.

nr 1

Reliable determination of hookworm nematode parasite species from dogs can be carried out post-mortem by microscopical examination of the buccal cavity of adult worms isolated from the host small intestine. In order to allow a proper diagnosis of the hookworm infection in a living host, we developed a polymerase chain reaction (PCR). Using DNA extracted from hookworm eggs and an oligonucleotide set designed on the basis of the DNA sequence of a cysteine proteinase AcCP1 gene, Ancylostoma caninum and Uncinaria stenocephala DNA can be discriminated from each other.

Ekspresja i oczyszczenie rekombinowanego bialka fuzyjnego GST-proteaza cysteinowa Ancylostoma ceylanicum

80%

Wisniewski M. , Mieszczanek J. , Wedrychowicz H.

Wiadomości Parazytologiczne

2004

tom 50

nr 3

491-494

Humoral response in hamsters following vaccination with cDNA encoding ACEY-1 cysteine proteinase of Ancylostoma ceylanicum

80%

Mieszczanek J. , Swiderska M. , Wedrychowicz H.

nr 4

The humoral response in hamsters following vaccination against Ancylostoma ceylanicum infections with DNA construct was investigated. Groups of hamsters were injected intramuscularly with plasmid pcDNA 3.1, containing cDNA of ACEY-1 cysteine proteinase. Vaccination resulted in IgG antibody response to somatic extracts of adult A. ceylanicum. The highest level of antibodies was observed seven weeks after vaccination.