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1

Effects of cadmium on seed germination, coleoptile growth, and root elongation of six pulses

100%

Tao L. , Guo M. , Ren J.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 1

EN

The ecotoxicological effects of Cd²⁺ on germination and early seedling growth of six pulses were investigated. Seeds of these plants were exposed to seven different concentrations of Cd (0, 0.1, 0.2, 0.4, 0.8, 1.6, and 3.2 mM). The results indicated that root and coleoptile growth of six pulse plants were more sensitive than seed germination for measurement of toxic Cd²⁺ pollution. Different species show different levels of tolerance to Cd²⁺ pollution. V. angularis and Dumasia villosa are the most sensitive to Cd²⁺, and their germination percentage, and root and coleoptile growth were significantly lower than other tested species. By contrast, Vigna radiata and Lablab purpureus are the most resistant species, their germination and seedling growth almost were not influenced by Cd²⁺ pollution significantly compared to control. There were significantly negative correlations between seedling growth and increasing concentrations of Cd²⁺ for V. angularis. V. angularis and Dumasia villosa are the most sensitive to Cd²⁺. By contrast, Vigna radiata and Lablab purpureus are the most resistant species.

2

Synthesis and characterization of nanoscale Bi2Cu0.1V0.9O5.35 powders by solution-based chemical methods

100%

Guo M. , Deng H. , Yang P.

Materials Science Poland

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2009

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tom Vol. 27, No. 4/1

1055--1066

EN

Nanocrystalline Bi2Cu0.1V0.9O5.35 (BCVO) powders were synthesized by solution-based chemical methods. Materials have been characterized by the thermogravimetric analysis, X-ray diffraction, and scanning/transmission electron microscopy. The sintering temperatures to complete phase transition are above 330 °C, 320 °C and 470 °C for the primary powders obtained by the sol-gel technique, an ethylenediaminetetra acetate-citrate combustion gel route and a modified reversing titration co-precipitation method (RP), respectively, which were much lower than the 600 °C required by conventional solid state method (SS). The powders synthesized by EC with 2 wt. % of surfactant, polyethylene glycol PEG 4000 have rather a narrow size distribution, within 30-50 nm, and as the PEG 4000 content increases, they form agglomerates irregular in shapes. In RP the optimal concentration of PEG 4000 is about 5 wt. % and the average grain size of the sample is 20 nm. The modified reaction in homogeneous solutions can be controlled to produce uniform BCVO nanoparticles.

3

Human-Friendly Design of Virtual System “female body-dress”

100%

Guo M. , Kuzmichev V. E. , Adolphe D. C.

Autex Research Journal

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2015

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tom Vol. 15, no. 1

19--29

EN

Recently, the development efforts focused on the computer simulation of garments, which depend on the material's physico-mechanical properties. It intends to achieve the best possible and realistic simulations of garments, which are available for pressure prediction. In this manner, 3D garment virtual technology improvements allow the visualization of pressure areas with values where the fabric might be too tight against the body. Although the purposes of simulation graphics were acceptable, the accuracy for apparel shaping is not enough to meet the needs of Virtual Prototyping and CAD utilization especially while the fabric properties system design was inadequate. Moreover, the existing pressure simulation is intended to simply predict the pressure index or how the textile deformation extend, which are deficient in real human's perception. In this research, the 3D shapes belonging to typical female bodies and dresses made of different fabrics were obtained by 3D body scanners (ScanWorX and TELMAT). Through reconstruction for the 3D torso shapes, the volumetric eases between body and dress were calculated by means of a software Rhinoceros. A new approach for the selection of textile properties based on the Kawabata Evaluation System (KES) was proposed to investigate its relations with dress shaping and pressure comfort. Finally, fabric properties tested by the KES-F system were compared with volumetric eases, objective pressure indexes and subjective comfort scores to reveal the relations how the fabric properties have impacts on dress outside shaping and inside pressure comfort of a female body. In this manner, the human-friendly CAD instead of mechanical approach existing before has been presented as a new approach to promote the construction of a realistic system for the 3D simulation optimization.

4

Application of grey model GM(1, 1) to ultra short-term predictions of universal time

88%

Lei Y. , Guo M. , Zhao D. , Cai H. , Hu D.

Artificial Satellites : Journal of Planetary Geodesy

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2016

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tom Vol. 51, No. 1

19--29

EN

A mathematical model known as one-order one-variable grey differential equation model GM(1, 1) has been herein employed successfully for the ultra short-term (<10days) predictions of universal time (UT1-UTC). The results of predictions are analyzed and compared with those obtained by other methods. It is shown that the accuracy of the predictions is comparable with that obtained by other prediction methods. The proposed method is able to yield an exact prediction even though only a few observations are provided. Hence it is very valuable in the case of a small size dataset since traditional methods, e.g., least-squares (LS) extrapolation, require longer data span to make a good forecast. In addition, these results can be obtained without making any assumption about an original dataset, and thus is of high reliability. Another advantage is that the developed method is easy to use. All these reveal a great potential of the GM(1, 1) model for UT1-UTC predictions.

5

ZNF300, a recently identified human transcription factor, activates the human IL-2Rbeta promoter through the overlapping ZNF300/EGR1 binding site

88%

Xue L. , Qiu H. , Ma J. , Guo M. , Li W.

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tom 15

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nr 4

EN

ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

6

Identification of the DNA binding element of the human ZNF300 protein

75%

Qiu H. , Xue L. , Gao L. , Shao H. , Wang D. , Guo M. , Li W.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 3

391-403

EN

The human ZNF300 gene is a member of the KRAB/C2H2 zinc finger gene family, the members of which are known to be involved in various developmental and pathological processes. Here, we show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. The ZNF300 DNA binding site, C(t/a)GGGGG(c/g)G, was defined via a random oligonucleotide selection assay, and the DNA binding site was further confirmed by electrophoretic mobility shift assays. A potential ZNF300 binding site was found in the promoter region of the human IL-2Rβ gene. The results of electrophoretic mobility shift assays indicated that ZNF300 bound to the ZNF300 binding site in the IL-2Rβ promoter in vitro. Transient co-transfection assays showed that ZNF300 could activate the IL-2Rβ promoter, and that the activation was abrogated by the mutation of residues in the ZNF300 binding site. Identifying the DNA binding site and characterizing the transcriptional regulation property of ZNF300 would provide critical insights into its potential as a transcriptional regulator.

7

Self-preserving lognormal volume-size distributions of starch granules in developing sweetpotatoes and modulation of their scale parameters by a starch synthase II (SSII)

75%

Guo M. , Xia Q. , Akwe A. W. , Stewart L. , Ashu G. M. , Njiti V.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 11

8

Transcriptome analysis reveals anthocyanin acts as a protectant in Begonia semperflorens under low temperature

75%

Bi H. , Guo M. , Wang J. , Qu J. , Du W. , Zhang K.

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nr 01

EN

Anthocyanins are natural bioactive pigments in plants that play important roles in many physiological functions. They are found in various tissues and can protect plants against different stress conditions. Anthocyanins are synthesized and accumulate in nutritional organs, which is crucial for plants to adapt to and resist adverse environmental conditions, including high exposure to light, ultraviolet light, low temperatures, drought, pests and disease. Some progress has been made in understanding the adaptability of anthocyanin to the external environment. Begonia semperflorens is an excellent model for studying the function and regulation of anthocyanin synthesis. To investigate the biosynthesis and regulation of anthocyanins, RNA sequencing techniques were employed to investigate anthocyanin biosynthesis induced by low temperature in B. semperflorens leaves. A total of 74,779 unigenes with a mean length of 1249 bp were assembled. Functional annotations were implemented using five protein databases. Differentially expressed genes involved in the process of anthocyanin biosynthesis were identified. This study represents the first report of a broad-scale gene expression study on B. semperflorens.