Wyniki wyszukiwania - Biblioteka Nauki

1

Antybiotyki - 'cudowne leki' przestaja byc skuteczne

100%

Binek M.

Magazyn Weterynaryjny

|

2009

|

tom 18

|

nr 12

1318-1323

2

Chorobotworczosc i wystepowanie E.coli serotyp O157 : H7

100%

Binek M.

|

nr 03

64-66

3

Contribution of veterinary mocrobiology to one health idea

100%

Binek M.

|

2017

|

tom 56

|

nr 3

4

Od postulatów Kocha do socjomikrobiologii

100%

Binek M.

|

tom 67

|

nr 03

EN

The year 2010 marks the 100th anniversary of Robert Koch’s death. His name is well known as one of the founders of modern microbiology, the discoverer of the etiologic agents of anthrax, tuberculosis, cholera and wound infection. What is equally important, he developed the methodologies and concepts that made microbiology a scientific discipline. He is best known for having developed the rules, or postulates, that determine the specific agent of an infectious disease. Koch’s postulates were a landmark in medical microbiology because they could be used to prove beyond any doubt that specific bacteria were the cause of an infectious disease. However, significant limitations to the postulates were soon recognized and restricted their wider scientific application. Now, over a century later, a more rigorous method to test causability still has to be developed. Technological advances led to the discovery of viruses, prions and new classes of bacteria that cannot fulfill Koch’s postulates. Advances in molecular microbiology have demonstrated that virtually all microorganisms can be considered opportunistic. They constantly turn their virulence genes on and off in a context-dependent manner to acquire unique phenotypes. They have evolved sophisticated mechanisms to sense changes in the environment and respond accordingly when their survival is threatened. Local environmental cues are gathered by bacteria at the membrane level and transmitted do deeper circuits within their cytoplasm, where they are processed by another sensing system, known as the quorum sensing signaling system. As a result of the processing of environmental information, the bacteria shift their phenotype to adjust better to the local conditions, resulting in either pathogenic or non-pathogenic behavior. Infectious pathogenesis is dictated by the chemical crosstalk between the complex network of bacteria- -bacteria, host-bacteria, and host-host. The ability of bacteria to intercommunicate and process information about resource availability and the health status of the host has led to the concept of bacteria behaving like a social group. The study of social networks developed among bacteria in their natural habitats is called sociomicrobiology.

5

Znaczenie jelitowych mikrobiontów w utrzymaniu ogólnej homeostazy gospodarza

100%

Binek M.

|

tom 54

|

nr 3

6

Bakteryjne czynniki zapalenia gruczolu mlekowego u krow

63%

Binek M. , Blaszczak B.

|

nr 06

291-295

7

Mechanizm zakazenia paleczkami Salmnonella

63%

Binek M. , Blaszczak B.

|

tom 45

|

nr 1

27-38

8

Czestosc wystepowania Lawsonia intracellularis u swin nie wykazujacych objawow chorobowych rozrostowego zapalenia jelit

63%

Plawinska-Czarnak J. , Binek M.

|

nr 11 Supl.

1497-1499

EN

The aim of the study was to evaluate the incidence of Lawsonia intracellularis in a population of healthy pigs and to determine the section of ileum most frequently inhabited by the microorganism. The investigation covered a total number of 832 healthy finishing pigs of similar age. The body weight of individuals ranged from 90 to 120 kg. The animals did not show any symptoms of the disease and they were directed to slaughter. Samples of ileum, caecum and colon were subjected to nested PCR test for the presence of L. intracellularis. The results showed that the bacteria mainly inhabits the ileum (33.3%). The p78 genome fragment of L. intracellularis was sporadically detected in the large intestine: 8.2% in colon and 5.4% in caecum samples. This specific fragment was found in 43.1% of intestinal samples of healthy slaughter pigs from different regions of Poland. The percentage of the herds infected was established at 73.4%.

9

Protective effect of potentially probiotic Lactobacillus strain on infection with pathogenic bacteria in chickens

63%

Kizerwetter-Swida M. , Binek M.

|

tom 12

|

nr 1

EN

The probiotic potential of a Lactobacillus salivarius 3d strain isolated from chicken faeces was assessed in one day old chickens. Lactobacillus salivarius 3d was administered per os at a concentration of 10⁸ cfu in 100 μl of PBS. The chickens were then challenged with pathogenic bacteria: Salmonella Enteritidis, Campylobacter jejuni and Clostridium perfringens. Samples of caecal contents and livers were collected after 1, 2, 3, 7 and 14 days after infection. Lactobacilli and pathogenic bacterial cell counts were determined in the samples. This study showed that L. salivarius 3d reduced the number of Salmonella Enteritidis and Clostridium perfringens in the group of chickens treated with Lactobacillus. Therefore it may be concluded that L. salivarius 3d may be used as a potential probiotic for chickens.

10

Zakazenia kurczat Salmonella enteritidis - znaczenie odpornosci komorkowej

63%

Binek M. , Blaszczak B.

Postępy Mikrobiologii

|

1995

|

tom 34

|

nr 4

403-416

11

Chicken intestinal microbiota function with a special emphasis on the role of probiotic bacteria

63%

Cisek A. A. , Binek M.

|

nr 2

EN

Bacterial colonization of the chicken gut by environmental microbes begins immediately after hatching. Composition of the intestinal microbiota is dependent on the surrounding environment, diet variation, pathological conditions, antibiotic therapy, and others. The genomes of all these intestinal microbes form a microbiome which by far outnumbers the host’s genome. As a consequence, the microbiome provides additional metabolic functions to the host, including nutrient utilization and absorption, fermentation of non-digestible dietary fiber, synthesis of some vitamins, biotransformation of bile acids, and the well-being of their chicken host. Microorganisms can also directly interact with the lining of the gastrointestinal tract, which may alter the physiology and immunological status of the bird. Since newly hatched broiler chickens demonstrate delayed commensal colonization and low bacterial diversity, the most effective and harmless method available to control the development and composition of the intestinal microbiota is a competitive exclusion treatment by applying probiotic bacteria. Additionally, recent research has shown that probiotic bacteria have a variety of beneficial effects, including counteraction of dysbiosis, promotion of gut health and homeostasis, enhancement of immune defenses and antagonization of infectious agents.

12

Zastosowanie sztucznych sieci neuronowych w identyfikacji parametrów wielkości zakłóceniowych

63%

Binek M. , Kanicki A.

|

2014

|

tom R. 90, nr 3

51--57

PL

Artykuł przedstawia wyniki identyfikacji parametrów wielkości zakłóceniowych występujących w systemie elektroenergetycznym. Oceniane będą wyniki otrzymane za pomocą trzech sztucznych sieci neuronowych oraz trzech klasycznych metod identyfikacji. Metody identyfikacji będą poprzedzone działaniem filtru cyfrowego typu Chebysheva II rzędu. Sygnałami wejściowymi w procesie identyfikacji będą funkcje analityczne opisujące typowe przebiegi wielkości zakłóceniowych występujących w systemie elektroenergetycznym. Sygnałem wyjściowym będzie wartość skuteczna sygnału wejściowego a kryteriami oceny będą błąd maksymalny oraz wartość średnia błędu w oknie pomiarowym.

EN

The article presents the results of parameter identification of the fault values found in an electrical system. The results obtained by means of three artificial neural networks and three classical methods of identific ation will be assessed. The identification methods will be preceded by a Chebyshev type II digital filter. The input signals in the identification process will be constituted by analytical functions describing typical waveforms of fault values found in an electrical system. The output value will be constituted by the RMS of the input signal and the maximum error and the average error of the measurement window will be the assessment criteria.

13

Znaczenie plazmidowego zgrupowania genow spv w chorobotworczosci paleczek Salmonella enteritidis dla kur. I. Wystepowanie zgrupowania genow spv w duzych plazmidach zjadliwosci paleczek Salmonella enteritidis

63%

Madajczak G. , Binek M.

|

nr 2

163-174

PL

Zakażenia pałeczkami Salmonella są powszechne wśród ludzi i zwierząt, występują na całym świecie, zarówno w krajach wysoko rozwiniętych, cechujących się znacznym poziomem higieny, jak i w krajach ubogich, z niedostateczna opieką zdrowotną. Z tych przyczyn salmoneloza jest nadal poważnym zagrożeniem dla zdrowia człowieka. Problem ten może okresowo urastać nawet do rangi politycznej, jak np. w przypadku wybuchu epidemii.

EN

Many Salmonella Enteritidis virulence factors are encoded by genes localized on plasmids, especially large virulence plasmid, in highly conserved fragment, they create spv plasmid gene group. The aims of realized researches were spv genes occurrence evaluation and composition analysis among Salmonella Enteritidis strains caused infection in chickens. Researches were realized on 107 isolates, where in every cases large virulence plasmid 59 kbp size were detected. Specific nucleotides sequences of spv genes (spvRABCD) were detected in 47,7% of isolates. In the rest of examinated bacterias spv genes occurred variably. Most often extreme genes of spv group, like spvR and spvD were absent, what could indicate that factors encoded by them are not most important for Salmonella Enteritidis live and their expressed virulence.

14

Wspolczesne metody diagnostyki laboratoryjnej chorob zakaznych

63%

Klimuszko D. , Binek M.

|

tom 81

|

nr 10

666-671

15

Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens

63%

Kizerwetter-Swida M. , Binek M.

|

nr 1

EN

This study was performed in order to isolate lactobacilli from chicken droppings and to select strains with the most promising probiotic properties. Lactobacillus strains were isolated from a flock of healthy laying hens. The first selection criterion was the ability to inhibit the growth of Salmonella Enteritidis. Then the tolerance to low pH and bile salt, the ability to coaggregate with pathogenic bacteria and hydrogen peroxide production were evaluated. Four isolates showing the best antagonistic activity against Salmonella Enetritidis were selected for further research. All isolates tested tolerated low pH and bile salt, likewise all produced hydrogen peroxide. They efficiently coaggregated with C. perfringens and relatively less with E. coli. Isolate 03’04 displayed above-average results in all criteria, thus it is considered as a potential probiotic for chickens, and will be further evaluated for health promoting effect in animals. The results presented in this study confirm the strain specific probiotic properties and prove the probiotic potential of isolate 03’04. Strong antagonistic properties against C. perfringens exhibited by certain Lactobacillus strains indicate the possibility to use them as a component of probiotic supplement in necrotic enteritis of poultry.

16

Zatrucie jadem kielbasianym - problem wciaz aktualny

63%

Kizerwetter-Swida M. , Binek M.

|

nr 2

75-85

17

Zakażenia pałeczkami Salmonella u niosek i brojlerów Gallus gallus, toksykoinfekcje pokarmowe u ludzi

63%

Binek M. , Szczawinski J.

Postępy Nauk Rolniczych

|

2008

|

tom 60

|

nr 2

18

Znaczenie plazmidowego zgrupowania genow spv u Salmonella enterica subsp.enterica

63%

Binek M. , Madajczak G.

|

nr 09

911-914

19

Komitet Nauk Weterynaryjnych PAN. Ocena dokonań nauk weterynaryjnych – mikrobiologia oraz choroby zakaźne i inwazyjne

63%

Kita J. , Binek M.

|

nr Zesz.Spec.

20

Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria

63%

Kizerwetter-Swida M. , Binek M.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr 4

EN

Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not always satisfactory. ITS-PCR together with protein profile were found to be helpful in strain identification. Lactobacilli were tested for the inhibitory activity against selected strains of poultry enteropathogenic bacteria (Salmonella Enteritidis, Escherichia coli and Clostridium perfringens). Examined supernatants from Lactobacillus broth cultures demonstrated major antimicrobial activity against C. perfringens. Lower antimicrobial activity were observed against E. coli and Salmonella Enteritidis. The strongest inhibition effect were obtained using supernatant of Lactobacillus acidophilus strain 3D. Results received from this study confirmed that identification of Lactobacillus spp. is often tedious. Some isolates, which are in vitro antagonistic against enteropathogenic bacteria may be considered as potential candidates for poultry probiotics, especially in controlling necrotic enteritis caused by C. perfringens.