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1 Journal of Medical Informatics & Technologies

1 2015

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Image analysis of immunohistochemical stains for detection of parathyroid disease

100%

Płaczek B. , Orczyk T. , Bułdak R. , Michalski M. , Segiet O. , Polaniak R.

tom Vol. 24

141--146

In this paper a method is introduced which enables automatic detection of parathyroid hyperplasia and parathyroid adenoma on the basis of immunohistochemical angiogenesis markers expression in micrographs. The proposed method uses digital image processing techniques and classification algorithms to detect diseased tissue. The disease detection is performed by classification of normalized color intensity histograms. Accuracy of this method was evaluated by using micrographs of parathyroid tissue sections obtained from patients that have undertaken surgery due to primary hyperparathyroidism. Use of different color models, various classifiers, and immunohistochemical markers was considered during the experiments. The experimental results show that the introduced method enables accurate detection of parathyroid disease. The most promising results were obtained for k-nearest neighbor and neural network classifiers.