Wyniki wyszukiwania - Biblioteka Nauki

1

May metallic biomaterials used for orthopaedic implants promote carcinogenesis? Preliminary transcriptomic research on human chondrocytes

100%

Walkowiak-Przybyło M.

Engineering of Biomaterials

|

2020

|

tom Vol. 23, no. 157

15--19

EN

The aim of this research was to assess the risk of carcinogenesis induced by the metallic materials intended for orthopaedic implants. The report is an analytical summary of changes in the expression of cancer-related genes in human chondrocytes of normal and neoplastic phenotype. Cq values (quantification cycle values) obtained from qRT-PCR reactions (quantitative real-time polymerase chain reactions) were used to count Fc values (fold change values) for each gene. Differences in Fc values obtained for primary and cancer cells grown on the surface of medical steel AISI316L and titanium-aluminum-vanadium alloy Ti6Al4V were then analyzed by t-Student test. The results indicate that for cancer cells grown on the surfaces of both examined materials the fold change greater than 2, usually considered essential, was found for LUM gene involved in sarcoma induction. For FOS gene, also involved in sarcoma induction, the Fc value was also very close to 2 in the primary cells exposed to Ti6Al4V alloy. The remaining observed changes were rather subtle, although they cannot be omitted from further studies because differences in gene expression in primary and tumor cells grown on the same biomaterial were statistically significant in several cases. The compilation of qRT-PCR experiments carried out on primary and cancer cells in parallel allowed to identify possible future contraindications for patients with a genetic predisposition to cancer or with cancer history.

2

Materiomika - pomost łączący naukę o materiałach z naukami o życiu

51%

Walkowiak B. , Walkowiak-Przybyło M. , Komorowski P.

|

tom nr 2

24--30

PL

Artykuł przedstawia najnowsze trendy w dziedzinie nauk o materiałach i związkach tej dziedziny nauki z naukami o życiu. Prezentuje także potencjalną możliwość wykorzystania komórki jako specyficznego sensora rozpoznającego produkty inżynierii materiałowej i nanotechnologii.

EN

This paper informs about the latest trends in th e field of materials science and the relationships of this field of science with life sciences. It also presents the potential of using a cell as a specific sensor that recognizes the products of materials engineering and nanotechnology.

3

May metallic biomaterials used for orthopedic implants promote cancer processes? : preliminary trasriptomics research on human articular chondrocytes.

45%

Walkowiak-Przybyło M. , Walczyńska M. , Kamińska M. , Komorowski P. , Walkowiak B.

Engineering of Biomaterials

|

2020

|

tom Vol. 23, no. 158 spec. iss.

32

4

Does surface structuring of metallic materials affect thrombocompatibility?

45%

Kamińska M. , Walczyńska M. , Walkowiak-Przybyło M. , Komorowski P. , Walkowiak B.

Engineering of Biomaterials

|

2020

|

tom Vol. 23, no. 158 spec. iss.

33

5

Commonly used metallic biomaterials may be involved in cancer processes – in vitro studies

38%

Walczyńska M. , Kamińska M. , Walkowiak-Przybyło M. , Siatkowska M. , Komorowski P. , Walkowiak B.

Engineering of Biomaterials

|

2024

|

tom vol. 27, no. 172

16--23

EN

There are reports available in the literature describing neoplastic changes around implants or at distant sites that temporally correlate with implantation, although they are not supported by sufficient clinical evidence. Such reports mainly concern the implantation of dental implants, which are performed in the largest number, and squamous cell carcinoma is one of the main types of cancer located in the vicinity of such implants. The occurrence of malignancies after hip arthroplasty has also been described in the vicinity of endoprostheses. At present, there are no indisputable data on the promotion of carcinogenesis by the implants used, and the problem of accelerated tumour induction in the area of implantation is still poorly understood and unclear. The aim of the study was a preliminary assessment of changes in the physiological processes of cells induced by metallic biomaterials intended for orthopaedic implants. A preliminary assessment of changes in the expression of cancer-promoting genes in chondrocytes exposed to metallic biomaterials was recently published. The current report is an analytical summary of changes in proliferation potential, DNA damage repair activity, and apoptosis level of primary and neoplastic cells (chondrocytes and osteoblasts) exposed to commonly used metallic biomaterials (AISI 316L, Ti6Al4V, Ti6Al7Nb, and CoCrMo). Immunofluorescence labelling techniques in flow cytometry were used for the study. The results obtained allow us to state that short-term (48 h) direct exposure to metallic biomaterials of osteoblasts and chondrocytes, both primary and cancerous, can cause significant changes in cell physiology, which may result in promoting the cancer process.