Badaniami objęto lipopolisacharydy wyekstrahowane z dziewięciu klinicznych szczepów B. fragilis wyizolowanych w Polsce. Biologiczną aktywność preparatów LPS oznaczono przy użyciu fotometrycznego testu BET (poprzednio LAL). Aktywność lipopolisacharydów klinicznych szczepów B. fragilis porównano z aktywnością LPS referencyjnych szczepów B. fragilis i LPS E. coli 055:B5. Wśród lipopolisacharydów pałeczek gatunku B. fragilis największą aktywność w reakcji z odczynnikiem LAL wykazał LPS szczepu wyizolowanego z ropnia trzustki.
EN
The aim of this study was to determine a biological activity of lipopolysaccharides (LPS) from clinical Bacteroides fragilis strains isolated in Poland by means of quantitative, photometric BET (LAL) method with Limulus polyphemus amoebocyte lysate and chromogenie substrate S-2423. Lipopolysaccharides were extracted from nine clinical B. fragilis strains by the procedure of Westphal and J arm (1965). Crude LPS preparations were purified with ultracentrifugation. Biological activities of bacterial endotoxins were determined by quantitative BET method with chromogenie substrate S-2423 (ENDOCHROME™ kit). Tests were performed according to the recommendations of the producer (Charles River Endosafe® Ltd., USA). E. coli 055:B5 LPS and LPS preparations from reference B. fragilis strains were applied to compare the results of examinations. Activities of endotoxins from clinical B. fragilis strains isolated in Poland determined in reaction with Limulus amoebocyte lysate were differentiated. Among endotoxins of clinical B. fragilis strains the most active was the preparation from strain cultured in the case of pancreatic ulcer (B. fragilis 80/81 LPS). Lipopolysaccharides of examined B. fragilis strains were less active in BET test than E. coli 055:B5 LPS.
Oznaczono zawartość glinu oraz podstawowych biopierwiastków Ca, Mg, Zn, Cu, Fe w kości udowej i wątrobie zwierząt doświadczalnych, u których wywoływano kumulację glinu w tkankach, a następnie poddano działaniu deferoksaminy. Poszukiwano zależności między stężeniem glinu a poziomem biopierwiastków w badanych tkankach oraz zmiany wywołane działaniem deferoksaminy - chelatora glinu.
EN
To assess the effect of aluminium intoxication in tissues of experimental animals on Ca, Mg, Zn, Cu, and Fe concentration, aluminium nitrate was administered intraperitoneally to mice at a daily dose of 0.27 mMol/kg for 5 weeks. Concentration of Al, Ca, Mg, Zn, Cu, and Fe were analyzed by atomic absorption spectrometry. The Al content in liver and tibia was significantly higher in treated mice in comparison with control group. In Al loaded tissues the significant increase of all tested essential elements was found. To evaluate the results of DFO treatment on essential elements, mice received 6 times intraperitoneally 3.5 mMol/kg of DFO. This treatment had generally no effect on reduction of Al concentration in tibia and liver, as well as on changes in essential elements concentration.
Przedmiot badań stanowiły lipopolisacharydy wyekstrahowane z ośmiu referencyjnych i dwóch klinicznych szczepów grupy B. fragilis (BFG). Biolo#giczną aktywność preparatów LPS oznaczono przy użyciu fotometrycznego testu BET (poprzednio LAL). Aktywność lipopolisacharydów pochodzących z pałeczek rodzaju Bacteroides porównano z aktywnością LPS E. coli O55:B5. Największy aktywnością w reakcji z odczynnikiem LAL odznaczały się lipopolisacharydy tych gatunków pałeczek BFG, które są ważne z klinicznego punktu widzenia - B. fragilis i B. thetaiotaomicron.
EN
The aim of this study was to determine and compare a biological activity of lipopolysaccharides (LPS) from reference and clinical strains of strictly anaerobic bacteria belonging to the Bacteroides fragilis group (BFG) by means of quantitative, photometric BET (LAL) method with Limulus polyphemus amoebocyte lysate and chromogenic substrate S-2423. Lipopolysaccharides of five BFG species were extracted by Westphal and Jann method (1965) from eight reference and two clinical strains of B. fragilis group. Crude LPS preparations were purified according to the procedure described by Gmeiner (1975) with ultracentrifugation and nuclease treatment. Biological activities of bacterial endotoxins were determined by quantitative BET method with chromogenie substrate S-2423 (ENDOCHROME™ kit, Charles River Endosafe® Ltd., USA). Tests were performed aoccording to the producer's recommendations. E. coli O55:B5 LPS was applied to compare its activity in reaction with LAL reagent with activities of LPS preparations from rods of the Bacteroides genus. Among examined bacterial compounds the most active in BET method was E. coli O55:B5 LPS. Activities of lipopolysaccharides from five species of BFG rods in reaction with Limulus amoebocyte lysate were differentiated. Greater ability to activate LAL proenzyme revealed lipopolysaccharides of these species of the Bacteroides genus, which are important from the clinical point of view - B. fragilis and B. thetaiotaomicron.