Wyniki wyszukiwania - Biblioteka Nauki

1

The application of spor culture in vegetative propagation of tree ferns of Cyathea genus

100%

Goller K. , Rybczynski J. J.

|

nr 2

139-143

EN

Multiplication of tree ferns using the traditional methods, i.e. the sowing of spores, is a very difficult task, characterised by low efficiency. It seems that in vitro culture of gamatophyte could be employed to overcome these difficulties. Experiments with vegetative propagation of tree ferns have been carried out in our lab over the last few years. The following species C. australis (R.Br), C. brownii (Domin), C. capensis, C. dealbata (G.Forst.)SW., C. dregei Kunze , C. giganthea, C. medullaris, C. smithii Hook f., C. spinulosa, Cyathea sp Sm were the subjects of our studies. Experiments were carried out with the use of two basal agar media: Murashige and Skoog (1962) and Knop medium. Various concentrations of minerals of the studied media were used. It was proved that the time required for the germination of sown spores on agar medium depended on the species and concentration of used mineral ingredients of the studied media. Finally, numerous plants were obtained only from C. australis and C. Cooperi.

2

Plant regeneration from immature inflorescence-derived callus of Italian ryegrass (Lolium multiflorum Lam.): chromosome number and fertility of regenerated plants

100%

Zwierzykowski Z. , Rybczynski J. J.

|

1998

|

tom 39

|

nr 3

217-224

EN

Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L-1 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L-1 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L-1 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x = 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.

3

Cryopreservation - a tool for long-term storage of cells, tissues and organs from in vitro culture derived

100%

Mikula A. , Rybczynski J. J.

|

2006

|

nr 4

145-163

EN

Cryopreservation offers the possibility for long-term storage of genetic resources with maximal genotypic stability, using a minimum of space and maintenance. At present it is actively used all over the world for storage of plant material: seeds, pollen, spores, dormant buds or apical meristems in genebanks. The development of biotechnology led to the production of a new category of germplasm for cryostorage: in vitro obtain tissues, organs, embryos, special cell lines and genetically modified plant material. The maintenance of in vitro collections remains risky regarding losing accessions due to the contamination, human error or somaclonal variation. The classical slow cooling was the first standard protocol developed for hydrated plant tissues. This method is mainly used for cryopreservation of non-organized tissues, for example: cell suspensions and calli, or apices of cold-tolerant species. For differentiated structures, new cryopreservation techniques such as vitrification and encapsulation/dehydration procedures or droplet method are efficient and reliable. These freezing techniques have been successfully, routinely applied for cryopresevation of various plant material of temperate and tropical climate species. So far, cryopreservation procedures are developed for in vitro tissues and recalcitrant seeds of about 100 and 40 species, respectively.

4

Present status of biotechnology of rye (Secale cereale L.)

100%

Bolesta E. , Rybczynski J. J.

|

2001

|

nr 4

55-73

EN

This review paper covers the results of tissue culture and biotechnology of rye (Secale cereale L.) published between 1990 and 2000. The following subjects were raised: somatic embryogenesis, haploid production, intergenetic hybridisation and transformation. Upon conclusion of all the results published to date, we may say that the progress of biotechnology in the case of rye is very limited in comparison to other cereals. However, a lot of work and efforts were involved to obtain the aforementioned result. Taking into account the progress in plant genetics and molecular biology, the authors deeply believe that the New Millennium will bring a brake-through in rye biotechnology.

5

Present status of Gentiana taxa biotechnology

100%

Mikula A. , Rybczynski J. J.

|

nr 4

76-91

EN

Data on the tissue culture and genetic manipulation of several species of the genus Gentiana in support on the review of literature are presented.