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2 Acta Physiologiae Plantarum

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The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.)

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Meng L.-S. , Song J.-P. , Sun S.-B. , Wang C.-Y.

nr 6

Carnation (Dianthus caryophyllus L.) is one of the most important ornamental plants in the world. Though morphological modification of carnation is very important to its commercial value, there have been no relevant reports until now. PttKN1 (Populus tremula × Tremuoides knotted1), isolated from the vascular cambial region of hybrid aspen, is a novel member ofKNOXgene family. In this paper, we transformed 35S:PttKN1 to carnation via Agrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and selfpollinated. A total of 32 T0 progeny with aberrant phenotypes were obtained. PCR assay proved the validity of these transgenic plants. Phenotypes of 32 35S:PttKN1 plants were distinct from those of wild-type plants, including: (1) modification of phyllotaxis (15/32): wild-type carnation was with typical opposite phyllotaxis, while transgenic plants displayed tricussate whorled and multiple-cussate whorled phyllotaxis. Irregular modification of phyllotaxis was also observed; (2) modification of stem (9/32): wild-type stems were round; however, some transgenic plants exhibited much thicker and flatter stem; (3) the whole transgenic plants of carnation (8/32) became dwarf. These morphological modifications of carnation indicate that we have successfully attained some novel lines of carnation. These lines can have potential practical applications. In conclusion, the selection of stably genetic lines is discussed.

ASYMMETRIC LEAVES2-LIKE38, one member of AS2/LOB gene family, involves in regulating ab-adaxial patterning in Arabidopsis lateral organs

100%

Wang Y.-B. , Song J.-P. , Wang Z.-B.

nr 09

ASYMMERTIC LEAVES1 (AS1) and AS2 in Arabidopsis are essential in determining leaf cell fates of adaxial axis. Here, we report ASYMMERTIC LEAVES2- LIKE38 (ASL38/LBD41), is an important gene of the LATERAL ORGAN BOUNDARY DOMAIN family. ASL38/ LBD41 was detected in the adaxial and internal domain between the ab-adaxial domains of leaves. For explaining ASL38/LBD41 role in Arabidopsis development, a construct of the sense-expressing ASL38/LBD41 was transformed to Arabidopsis (Col-0); thus gained both overexpressing and cosuppressing 35S:ASL38/LBD41 plants. Rosette leaves of these overexpressing plants showed narrow (Class I) to radial symmetric needle-like patterns (Class II), compared with those of wild-type control, exhibiting adaxilized defect. However, rosette leaves of these cosuppressing plants showed narrow (Class I) to radial symmetric needle-like patterns (Class II), in contrast to those of wild-type (Col-0), exhibiting abaxilized defect. Furthermore, phenotypes observed in asl38-1 mutants suggest a redundant function for ASL38/LBD41 in boosting adaxial and/or inhibiting cell fate in abaxial axis. Together, our data suggest that ASL38/LBD41 might play a role of specialization in adaxial cell fate in Arabidopsis lateral organs.