Wyniki wyszukiwania - Biblioteka Nauki

1

Rola Campylobacter w patologii drobiu i czlowieka

100%

Wieliczko A.

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nr 1-2

4-9

2

Rola Campylobacter w patologii ptakow. II. Badania nad okresleniem obecnosci czynnikow wirulentnych u szczepow Campylobacter jejuni

100%

Wieliczko A.

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nr 10

614-618

EN

The present study was carried out to determine the presence of virulent factors in C. jejuni isolated from the alimentary tracts and livers of layer and broiler chickens. A tissue culture assay in vivo and invasivenes test in vitro on chicken embryos to detect the presence of the virulent factors mentioned above. It was found that the polymixyn extract of C. jejuni exhibited cytotoxic effects which were most clear in chicken kidney cells (94% of C. jejuni: the titer ranged from 4 log 2 to 12 log 2). Chicken embryo fibroblasts were less sensitive to Campylobacter filtrates (84% of C. jejuni: the titer ranged from 1 log 2 to 9 log 2). The lowest sensitivity was found in Vero cells. The invasive factor was determined in C. jejuni after the inoculation of 11-day-old chicken embryos onto the chorioallantoic membrane and into the allantoic cavity. There was a correlation between mortality of embryos and reisolation C. jejuni from their livers. However, this relationship was not observed after the inoculation of the embryos into the allantoic cavity.

3

Zakazenia drobiu Ornithobacterium rhinotracheale

100%

Wieliczko A.

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nr 10

532-535

4

Zakazenia kur paleczkami Salmonella Gallinarum-Pullorum [tyfus kur]

63%

Kuczkowski M. , Wieliczko A.

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tom Supl

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nr Drob

23-25

5

Kampylobakterioza zwierzat - aspekty epidemiologiczne

63%

Bednarski M. , Wieliczko A.

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nr 11

1211-1214

EN

Campylobacter sp. may colonize the intestinal tract of most mammal and bird species. The resultant infections are symptomless or can cause diarrhea. When they concern humans, they are a serious epidemiological problem. In developed countries campylobacteriosis is now one of the most frequent reasons for food-borne diseases and is usually associated with gastroenteritis. In rare cases the infection may lead to complications like septicemia or Gullain-Barre syndrome. Campylobacteriosis is a zoonozis, therefore animal food products are the main source of the infection of Campylobacter for humans. As a zoonozis, in Poland all cases of campylobacteriosis in animals must be registered.

6

Charakterystyka molekularna krajowych szczepów cirkowirusów izolowanych od gęsi

51%

Kuczkowski M. , Piasecki T. , Wozniak A. , Wieliczko A.

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nr 10

EN

Goose circovirus (GoCV) infections are commonly found in geese on European and Asian farms, but the role of GoCV as a causative agent of the problem in geese production has not been established yet. The aim of the study was to investigate the prevalence and molecular characteristics of circovirus infection in commercial geese farms located in South-East Poland. Tissue samples of spleen or bursa of Fabricius from 95 dead geese of different ages have been collected from 28 farms/flocks between April and September 2005. None of the clinical signs and important problems with production were reported during the time of the investigation. A polymerase chain reaction (PCR) was designed for the diagnosis of the infection. From positive samples a C1 gene fragment which encodes the capsid protein (after translation of 250 amino sizes), using two pair of primers has been amplified and a sequencing reaction was performed in both directions. The nucleotide sequences were aligned with the sequences geese circovirus strains obtained from GeneBank by BioEdit sequence alignment editor software using Clustal W multiple alignment algorithm. Prevalence of GoCV was found in 18 (18.9%) spleens or bursa of Fabricius samples from geese from 14 (35.0) flocks. All positive birds were more than 5-weeks-old. The nucleotide sequence of C1 gene fragment of Polish GoCV showed 99% to 84% identity with C1 gene sequenced by Todd et al. (accession number AJ304456 GenBank database).

7

Zachowanie się subpopulacji limfocytów T CD3+, CD4+ i CD8+ u kurcząt w przebiegu zakażenia Salmonella Enteritidis

51%

Wieliczko A. , Kuczkowski M. , Mazurkiewicz M.

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nr 07

8

The clinical course of the infectious laryngotracheitis (ILT) field case in hens and estimation of immunoprophylaxis efficiency

51%

Wieliczko A. , Mazurkiewicz M. , Piasecki T. , Kuszczynski T.

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nr 2

9

Zakazenia cirkowirusowe u ptakow

51%

Wieliczko A. , Kuczkowski M. , Piasecki T. , Wozniak A.

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nr 03

282-285

EN

Circovirus infections are commonly found in birds and mammals. Today, two genuses: Gyrovirus and Circovirus occur within the Circoviridae family. In poultry pathology, especially on large commercial farms of hens and chickens, special attention is focused on Chicken Infectious Anemia Virus-CIAV of the Gyrovirus genus. The genus of Circovirus includes Psittacine Beak and Feather Disease Virus (PBFDV), pigeon circovirus, referred to as Columbid circovirus (CoCV), or Pigeon cicovirus (PiCV), goose circovirus (GoCV), canary circovirus (CaCV) and others described as circovirus-like diseases, isolated from other species of birds. The paper presents characteristics of circoviruses and the immunosuppressive effects on host cells as well as the diagnosis of infections in birds.

10

Antimicrobial susceptibility of Salmonella spp.strains isolated from free-living birds

51%

Krawiec M. , Rusiecki S. , Kuczkowski M. , Wieliczko A.

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nr 4

11

Kolibakterioza ptakow - istotny problem w produkcji drobiarskiej

51%

Wieliczko A. , Piasecki T.

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nr 03

31-32

12

Nosicielstwo i siewstwo Salmonella typhimurium u eksperymentalnie zakażonych kur niosek

45%

Zalesinski A. , Mazurkiewicz M. , Molenda J. , Wieliczko A. , Molenda O.

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nr 07

EN

The experiments were done on 60 laying hens (Astra S) aging 26 weeks and of an egg production about 50°/». The birds were inoculated perorally for 3 consecutive days using 2 ml of 18h borth culture of S. typhimurium. The following examinations were performed: clinical observations, egg laying, weekly bacteriological examinations of fecal swabs and eggs, serological examinations of blood sera and egg yolks by the tube agglutination test (OA) and by antiglobulin test (OAG). Moreover every 1—3 weeks 5 randomely chosen birds were sacrified and bacteriolo- gically examined. Hens infected perorally with S. typhimurium were clinically normal. The infections does not affected egg laying. S. typhimurium was isolated from internal organs only after 7 (100%' of birds) and 14 (40% of birds) after infection, and from intestines after 1 (80%), 2 (40%), 3 (40%) and 4 (20%) weeks after infection. The greates number of positive results for fecal swabs was noted after 1 (66%) and 6 (48%) weeks and from eggs after 1 (32%), 4 (32.5%), 5 (82.1%), 6 (81.4%), 7 (44.4%) and 8 (10.7%) weeks after infection. Diagnostically significant titres of serum antibodies in the OA test persisted for 3—8 weeks, and in the OAG test from 3 to 20 week. Similar tendencies but at low titres were noted for egg yolks. It was found that in laying hens perorally infected with S. typhimurium the bacterium persists in the organism and that it is disseminated for about 4 months. In bacteriological diagnosis better results are obtained in egg examinations than in fecal swabs examinations.

13

Porównanie antybakteryjnej aktywności fotodynamicznej in vitro protoporfiryny i fotolonu

39%

Ulatowska-Jarża A. , Borkowska J. , Kościelniak K. , Hołowacz I. , Wieliczko A. , Stręk W. , Podbielska H.

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tom Vol. 13, nr 4

361-367

PL

Celem pracy było zbadanie wrażliwości bakterii Salmonella enteritidis i Staphylococcus aureus na oddziaływanie foto-dynamiczne in vitro przy zastosowaniu dwóch różnych fo-touczulaczy: protoporfiryny PPIX i fotolonu w stężeniach 0,5%, 3,5%, 5,2%, 10%. Bakterie gram-ujemne (Salmonella enteritidis) są bardziej czule na promieniowanie laserowe o długości fali 662 nm niż bakterie gram-dodatnie (Staphylococcus aureus). Aktywność fotodynamiczna zależy od stężenia fotouczulacza i czasu naświetlania. Fotolon w wyższych stężeniach działa słabiej niż w niższych, natomiast protoporfiryna w wyższych stężeniach działa bardziej foto-cytotoksycznie.

EN

The aim of this work was to examine the in vitro sensitivity of Salmonella Enteritidis and Staphylococcus aureus to photodynamic activity of Protoporphyrine PPIX and fotolon in various concentrations: 0,5%, 3,5%, 5,2%, 10%. The Gram- bacteria (Salmonella Enteritidis) were more sensitive to 662nm laser radiation than Gram-I- bacteria (Staphylococcus aureus). Photodynamic activity depends on the concentration of photosensitizer and exposure time. Fotolon in higher concentration exhibits weaker photodynamic activity, whereas Protoporphyrine in higher concentrations is more photocytotoxic.

14

Skutecznosc preparatu - Aescotrim Suspension w zwalczaniu chorob bakteryjnych u drobiu

39%

Mazurkiewicz M. , Zalesinski A. , Wieliczko A. , Latala A. , Januszewski J. , Molenda O.

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tom 52

129-137

15

Wplyw probiotykow-Biogen Dw i Biogen Dp na efekty produkcyjne i wskazniki fizjologiczne kurczat rzeznych

39%

Mazurkiewicz M. , Jamroz D. , Gawel A. , Wieliczko A. , Klimentowski S. , Madej J. A.

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nr 08

368-371

EN

Two experiments were performed on slaughter cockerel chicks (Astra B2) to establish the influence of probiotics Biogen Dw (periodically applied in drinking water) and Biogen Dp (added to standard mixed feeds at a dose of 0,5 kg/ton), on productional effects (dynamic of growth, index of food conversion, healthy state) and on the following physiological parameters: morphology of peripheral blood, content of serum Ca, P, Mg, uric acid, immunoglobulins, total proteins and electrophoretic protein freactions, activity of microphages (test Chl and NBT), activity of serum enzymes — AspAT, AlAT, AcP, AT and the level of postvaccination immunity against ND, and morphology of internal organs (liver, spleen, kidneys, pancreas, heart, bursa of Fabricius, duodenum and ileum) and carrier state of Salmonella. It was found a positive influence of the used probiotics on dynamic of growth of chickens and on their healthy state. Birds receiving Biofen Dw characterized a higher slaughter body weight by 3.28% and a better index of food conversion by 1.2%. In chickens receiving Biogen Dp these values increased by 3.7—4.4% and 1.7—5.8%, respectively. Blood parameters were not affected. Only a slight increase in parameters of cellular immunity and in postvaccination immunity against ND, and a lack of Salmonella enteritidis carrier state was observed in birds after the used probiotics. The probiotics do not caused significant morphologic changes in the examined internal organs. Increased only the number of follicles in the liver and ileum wall without signs of stimulation.