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Cizaprid i prokinetyki nowej generacji w medycynie weterynaryjnej

100%

Skobowiat C. , Bossowska A. , Wojtkiewicz J. , Majewski M.

nr 02

142-144

Denervation of the porcine ovaries performed during the early luteal phase influenced morphology and function of the gonad

75%

Jana B. , Dzienis A. , Panczyszyn J. , Rogozinska A. , Wojtkiewicz J. , Skobowiat C. , Majewski M.

nr 1

Sympathetic innervation of vestigial uterus of the European bison bulls

63%

Skobowiat C. , Panasiewicz G. , Szafranska B. , Sidelnikow S. , Bieniek M. , Zamojska A. , Wardzynska M. , Gizejewski Z.

nr 3

This study describes the expression of DßH as a marker of sympathetic (catecholaminergic) innervation in the vestigial uterus of adult European bison (Eb) bulls. Cryo-sectioned uteri were examined by fluorescent immunohistochemistry with the use of primary rabbit DßH polyclonal antibodies. The DßH-immunocomplexes (antigens/polyclonals) were visualised by secondary donkey antirabbit biotinylated IγG, and then with cyanine (CY™3)-conjugated streptavidin. The DßH immunodetection with CY™3 for the Eb uterine sections was performed in parallel to a positive control (porcine sympathetic paravertebral ganglion), and a negative control (without primary anti-DßH polyclonals). This is the first paper describing the identification of DßH expression within the vestigial uterus of the adult Eb bulls. The distribution pattern of DßH expression localised within immunoreactive (IR) uterine nerve fibres of the bulls resembled that in the control female uterus. The DßH-IR nerve fibres were identified in the entire cross-section of each uterus, with generally higher density in the myometrium than in the endometrium. The ratio of the endometrial/myometrial DßH-expression (percentage of area with IR-signals) was comparable in all males and females (0.78 and 0.64, respectively). However, the DßH-expression area (%) was significantly lower within the male uterine endometrial (P<0.01) and myometrial regions (P<0.001) comparing to the female counterparts. Presumably, the DßH-IR expression within the male Eb uterus is associated with a trophic effect of noradrenaline released by sympathetic nerve fibres influencing nutrient supply of this vestigial organ in the Eb bulls.