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Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

100%

Stoynova-Bakalova E. , Nikolova M. , Maksymiec W.

2009

tom 51

nr 2

77-83

This study examined the effects of cytokinins and methyl jasmonate (JAMe) alone and in combination on the growth and flavonol content of zucchini cotyledons cultured in the presence or absence of Cu2+. During greening of cotyledons in intensive diurnal illumination, rutin was identified as the main flavonol compound. Its accumulation was greatly stimulated by phenylurea cytokinin (4PU-30) but reduced by Cu2+. Stimulation by benzylaminopurine (BA) was less. JAMe showed an inhibitory effect, alone and with simultaneous addition of Cu2+ or cytokinins. In moderate excess (100 μM and 250 μM CuSO4), Cu2+ enhanced stimulation by 4PU-30 of rutin accumulation; at a higher concentration or with other substances it decreased the rutin level. The other flavonol detected, kaempferol-3-rhamnoside, increased after JAMe or 4PU-30 were added; in the other cases it decreased. The data obtained indicate that cytokinins can increase rutin content in developing Cucurbita cotyledons. Rutin content usually decreased under stress induced by excess Cu2+, but Cu2+ in moderate excess had a stimulating effect in the presence of higher phenylurea cytokinin levels

Herbicide potential of selected essential oils from plants of Lamiaceae and Asteraceae families

75%

Nikolova M. , Traykova B. , Yankova-Tsvetkova E. , Stefanova T. , Dzhurmanski A. , Aneva I. , Berkov S.

tom 74

nr 1