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tom 48
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nr 02
81-83
EN
The purpose of the work was to determine the role of contaminated environment by U. diversum as a source of infections of the reproductive tract in cattle. It has been found that the period of Ureaplasma microorganisms survival outside the animals is relatively short, i.e. within 24 hours limits. However, it is long enough to make passible infection of the preputial mucosa of bulls. In contrast, heifers are rare infected under those conditions. Carries of U. diversum play an important role in the infection; their faeces sustain a permanent infection of environment. It constitutes a source of infection for animals free from infection. For prophylactic purposes one should take into account the need of carries removal by use of chemotherapeutics. Besides, an important role plays hygiene of cowsheds.
EN
Trial to infect bulls using Ureaplasma diversum culture (U) showed that this microorganisms regularly colonizes mucosal membranes of the urethra and preputial sac. More distal parts of the urogenital tract are colonized accidentally or the concentration of ureaplasms is low and therefore, it is difficult to detect ureaplasms. Appearance in 8 out of 12 infected bulls clinical signs of granular balanoposthitis (balanoposthitis granularis latin) poits to the role of U. diversum in etiology of this disease. Because this isolate of U. diversum caused also in cows granular vulvovaginitis (vulvovaginitis granularis latin) it was assumed that these two forms reflect one disease.
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nr 06
363-366
EN
The article presents the results of serological surveys of brucellosis conducted in 1998 on cattle, pigs, sheep, goats, hares, wild boars and dogs. No positive sero-reagents among the pig population were ascertained. The rate of positive results in cattle was established as 0.00098. When B. abortus antigen was used, all serum samples from sheep and goats reacted negatively. When B. ovis antigen was used, 0.91% of sheep sera were positive. The presence of anti-Brucella antibodies was demonstrated in hares, wild boars and dogs.
EN
In view of the large scale of surveys, the diagnosis of brucellosis is primarily based on serological examinations. Regarding cattle, RBT, SAT, CFT, Coombs, 2-ME and ELISA are used in Poland. The process is laborious and time-consuming. The problem of differentiation between positive reactions caused by Brucella and antigenically cross-reactive bacteria remains unresolved. The aim of the study was an application of a fluorescence polarization assay (FPA) for the examinations of sera from cattle for brucellosis. Four hundred fifty sera from cattle, including 300 sera from healthy animals, 27 sera from infected animals and 123 sera originated from confirmatory investigations, were used. The results obtained in FPA were compared to the results of RBT, SAT, CFT and ELISA. All sera from healthy animals were negative in FPA, whereas sera from infected animals were positive. Among sera from confirmatory investigations, 8 sera were positive in FPA. This likewise concerned sera positive both in CFT and ELISA. All sera positive only in RBT and SAT were negative in FPA. The results of the examinations show that FPA is a useful method for the diagnosis of brucellosis in cattle.
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