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1
Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils
100%
Budzynska A. , Saowska B. , Wieckowska-Szakiel M. , Rozalska B.
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tom 61
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nr 1
EN
 The influence of essential oils (EOs) used at sublethal level, on the presence and intensity of Candida albicans virulence factors was evaluated. Minimal inhibitory concentrations (MICs) of Lemon balm, Citronella, Geranium and Clove oils were established as 0.097% (v/v). Using the agar plates with substrates for proteases, phospholipases and hemolysins it was shown that C. albicans ATCC 10231 and C. albicans ATCC 90028 strains differed in the type and amount of enzymes produced. No significant difference in their total amount could be detected after pretreatment for 24 h with EOs at ½ MIC. However, the short-term (1 h) acting oils at MIC caused a statistically significant reduction in this activity. In the API ZYM test it was demonstrated that both strains exhibited activity of the same 9 out of 19 enzyme types and that EOs caused a significant decrease in the release of some of them. In the presence of subMIC of EOs, or when the fungus had previously been exposed to the MIC of oil, germ tubes formation was significantly and irreversibly reduced. Such C. albicans spotted on the Spider agar containing EOs at subMICs were unable to penetrate the agar. A significant decrease in the C. albicans adhesion to the fibroblast monolayer with respect to controls was also demonstrated when yeasts had been exposed to EOs at MIC (1 h) in liquid medium. Thus, it has been shown that tested oils, used even at subMIC, exhibit significant activity reducing the presence/quantity of important C. albicans virulence factors.
2
Aktywnosc indolicydyny, osobno lub w polaczeniu z oksacylina, wobec Staphylococcus aureus i Staphylococcus epidermidis
100%
Walencka E. , Sadowska B. , Wieckowska-Szakiel M. , Rozalska B.
Medycyna Doświadczalna i Mikrobiologia
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2008
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tom 60
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nr 3
191-196
3
Antimicrobial activity of essential oil and hydrolate from Myrica gale L. leaves
100%
Wieckowska-Szakiel M. , Napieralska K. , Budzynska A. , Kalemba D.
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2017
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tom 56
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nr 1
4
Analiza in vitro skuteczności modyfikowanych olejkami eterycznymi opatrunków absorpcyjnych wobec Staphylococcus aureus i Candida albicans
100%
Budzynska A. , Sadowska B. , Wieckowska-Szakiel M. , Rozalska B.
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nr 2
5
Staphylokinase production by clinical Staphylococcus aureus strains
100%
Wieckowska-Szakiel M. , Sadowska B. , Rozalska B.
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tom 56
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nr 2
EN
One of virulence factors produced by Staphylococcus aureus is staphylokinase (SAK), which enhances their proteolytic activity leading to tissue damage and improving bacterial invasiveness. In the present study we estimated the ability to produce staphylokinase by 95 S. aureus reference strains and clinical isolates from the airways of cystic fibrosis patients, from skin lesions and from infected bones. We would like to verify any relationship between SAK production and the types of clinical isolates as well as other biochemical properties and activities of these staphylococcal strains, which can be important for their pathogenicity. More than 62% of all tested strains were able to produce secreted type of SAK. Staphylokinase production was significantly more common in the isolates from skin and soft tissue infections than in any other group of tested staphylococci. The general tendencies in the selected properties or activities of both SAK(-) and SAK(+) isolates were similar. Our data confirm phenotypic dissimilarity in SAK production of S. aureus strains isolated from various types of infections. It is compatible with the biological role of staphylokinase and with hypothetical model of staphylokinase mediated bacterial invasion of host tissues. Thus, the estimation of SAK production by S. aureus isolates may be regarded as the parameter describing potential invasiveness of staphylococci and can be useful as a medical recommendation for the eradication of staphylococci carrier state.
6
Weryfikacja metodycznych parametrow oznaczania przeciwbakteryjnej aktywnosci olejkow eterycznych
88%
Budzynska A. , Wieckowska-Szakiel M. , Kalemba D. , Sadowska B. , Rozalska B.
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nr 3
281-287
7
How to enhance the effectiveness of antibiotics?
88%
Sadowska B. , Wieckowska-Szakiel M. , Budzynska A. , Zuchowski J. , Rozalska B.
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2017
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tom 56
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nr 1
8
Phenotypic and molecular characteristics of typical and atypical Escherichia coli O157, clinical and food isolates
75%
Sadowska B. , Osek J. , Bonar A. , Wieckowska-Szakiel M. , Rudnicka W. , Rozalska B.
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nr 2
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