Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 9

Liczba wyników na stronie
first rewind previous Strona / 1 next fast forward last
Wyniki wyszukiwania
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 1 next fast forward last
EN
Atherosclerosis, a chronic inflammation state of the aorta, is characterised by increased levels of pro-inflammatory cytokines (tumour necrosis factor alpha (TNFα), interleukin (IL)-1β, IL-6). Sheep is used in both cardiovascular and immunological studies; besides, ‘long-day’ ewe can be a model of leptin resistance state. The aim of the study was to examine whether photoperiodic conditions (long-day (LD) and short-day (SD) seasons) are a key factor modulating exogenous leptin influence on pro-inflammatory cytokines and their receptors gene expression in aorta of ewe’s with or without prior induction of acute inflammation. The experiment was conducted on 48 ewes during SD and LD seasons which were randomly divided into 4 groups: control; with LPS injection (400 ng/kg of body weight (BW)); with leptin injection (20 μg/kg BW); and with LPS and 30-min later leptin injection. Three hours after LPS/control treatment animals were euthanized to collect the thoracic aorta samples. In both seasons leptin injection intensified LPS-induced increase in IL1B gene expression but only in SD season leptin injection increased IL1R1 and IL1R2 gene expressions. The leptin injection increased IL6 gene expression but only in SD season. In the LD season leptin enhanced the LPS effect on IL6 gene expression. Neither TNFA nor its receptors gene expression was influenced by leptin regardless of season. In the thoracic aorta tissue an exogenous leptin exerts effect on pro-inflammatory cytokines and their receptors gene expression; however in ewe this influence depends on photoperiodic conditions. Moreover, leptin can moderate progression of the inflammation reaction in this tissue.
EN
The experiment was performed according to a 2 x 2 factorial design with breed and level of nutrition as factors affecting fat metabolism in pigs. Two groups of gilts, each comprising 4 Polish Large White (PLW) and 4 Synthetic Line 990 (L990) animals, were fed from 60 to 105 kg body weight on the experimental diet at 85% or 95% of assumed ad libitum intake. The diet contained 2% of linseed, 0.5% rapeseed and 0.5% fish oils as the source of n-3 fatty acids (FA). The carcass protein content was smaller and backfat thickness greater in L990 than in PLW pigs. Also the intramuscular fat content in the biceps femoris (BF) and longissimus dorsi (LD) muscles and their contents of total FA, saturated fatty acids, monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were higher or tended to be higher in L990 than in PLW pigs, the differences between the breeds being more pronounced in the BF muscle. The n-6/n-3 PUFA ratio tended to be lower in the LD muscle, whereas in the BF muscle, it was lower in L990 than in PLW pigs. Feeding at the 85% level resulted in smaller backfat thickness and carcass fat content in animals of both breeds. Gene expression of stearoyl-CoA desaturase in both muscles was higher in L990 pigs, which could have resulted in the higher MUFA and PUFA contents in this breed. Gene expression of fatty acid-binding protein 4 and peroxisome proliferator-activated receptor were affected both by breed and feeding level only in the BF muscle.
first rewind previous Strona / 1 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.