Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

2 2004

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Plant protoplast technology: current status

100%

Davey M. R. , Anthony P. , Lowe K. C. , Power J. B.

Acta Physiologiae Plantarum

2004

tom 26

nr 3 suppl.

Agrobacterium-mediated transformation of Bangladeshi Indica rices

88%

Al-Forkan M. , Power J. B. , Anthony P. , Lowe K. C. , Davey M. R.

2004

tom 09

nr 2

Morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the Bangladeshi Indica rice cultivars BR26 and Binni with Agrobacterium tumefaciens strain LBA4404 carrying the super binary vector pTOK233. Acetosyringone (100 μM) in the medium during coculture (25-28°C) and selection on hygromycin B (50 mg 1-1) were essential for efficient transformation. Stable integration and expression of β-glucuronidase, neomycin phosphotransferase and hygromycin phosphotransferase genes in regenerated plants were confirmed by histochemical and fluorometric assays, ELISA and Southern analysis. Two to 3 copies of T-DNA were integrated into regenerated plants; transgene expression did not correlate with gene copy number. Mendelian segregation of transgenes occurred in T1 seed progeny.