Wyniki wyszukiwania - Biblioteka Nauki

1

Effects of aluminum on superoxide dismutase and peroxidase activities, and lipid peroxidation in the roots and calluses of soybeans differing in aluminum tolerance

100%

Du B. , Nian H. , Zhang Z. , Yang C.

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nr 5

EN

The seedlings of two soybean genotypes, Al-tolerant PI 416937 (PI) and Al-sensitive Young, were cultured in the solution containing 0, 25 or 50 µM Al (AlCl₃‧6H₂O) for 24, 36 or 48 h in the hydroponics, and the calluses induced from two genotypes were cultured in medium containing 0, 10, 50 or 100 µM Al for 5, 10 or 15 days, respectively. The effects of Al on growth of seedling roots and calluses, antioxidant enzyme activities of superoxide dismutase (SOD) and peroxidase (POD) and lipid peroxidation were investigated. Under Al stress, PI was more tolerant to Al toxicity than Young at both intact plant and tissue levels and lower concentrations of Al significantly stimulated the root and callus growth of PI. Al application enhanced the activities of SOD and POD and lipid peroxidation in both roots and calluses of two genotypes. Although the differences of SOD activities between two genotypes in response to Al toxicity depended on Al concentration and durations of treatment, SOD activities in the roots of PI were higher than those in the roots of corresponding Young in the presence of Al for 36 or 48 h. Meanwhile, the POD activities in PI roots increased as the Al levels and durations of treatment increased, significantly higher than those in the corresponding Young roots. Moreover, Al-treated PI had significantly lower lipid peroxidation than Young at both root and callus levels. These results suggest that the enhanced antioxidant-related enzyme activities and reduced lipid peroxidation in PI might be one of Al-tolerant mechanisms.

2

Fast and highly sensitive spectrophotometric determination of protein in urine applying novel spectrum probe

88%

Wei Q. , Li Y. , Wang S. , Li Z. , Du B.

Chemia Analityczna

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2006

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tom Vol. 51, No. 1

159-171

EN

Molybdenurn(Vl)-trimethoxyphenylfluorone (TM-PF) complex has been used in spectro-photometric determination of protein in urine. At pH 2.43 and in the presence of Triton X-100 microemuision protein binds the complex within 2 min, which results in a maximum decrease of its absorbance at 527 nm. The presence of Triton X-100 microemuision significantly improves the sensitivity of the determination. The calibration plots for bovine serum albumin (BS A) and human serum albumin (HSA) were linear up to the analyte concentration of 11 μg mL-1> and 25 μgg ml-1>, respectively, with the corresponding molar coefficients of 1.13 x 107> L mol-1> cm-1> and 7.48 x 106> L mol-1> cm-1>. The proposed method is simple, practical, and relatively free from interference of coexisting substances, as well as much more sensitive than the Bradford method. Recovery of protein from human urine equals 96.7%-108% and the relative standard deviation is lower than 3.9%. The molar ratio method and the Rosenthanl graphic method have been employed to estimate the binding number and association constant of bovine serum albumin with the complex.

PL

Do oznaczania białek w moczu zastosowano kompleks molibdenu(Vl) z trimetoksyfenyloflu-oronem, do którego białko przyłącza się w ciągu 2 min przy pH 2.43 i \\ obecności Tritonu X-100. Największy spadek absorbancji następuje przy 527 nm. Obecność Tritonu X-100 znacznie poprawia czuiość oznaczania. Krzywe kalibrowania w pr/.ypadku albumin BSA i HAS były liniowe w zakresie, odpowiednio: do 11 μg mL-1> i 25 μg mL-1>, a współczynniki molowe absorbancji wynosiły 1,13 x 107> i 7,48 x I06> L mol-1> cm-1>. Zaproponowana metoda jest prosta, praktyczna i stosunkowo wolna od wpływu współobecnych substancji. Jest również bardziej czulą niż metoda Bradford'a. Odzysk białka/ moczu ludzkiego wynosił96.7-108 %. a względne odchylenie standardowe było mniejsze niż 3,9%. Do wyznaczenia stechiometrii tworzonych ukiadów i ich staiych trwałości zastosowano metodę stosunków molowych i graficzna metodę Rosenthanl'a.

3

Fast determination of ultra trace amounts of residual proteins in penicillin based on the enhancement in Rayleight light scattering spectra of phenylfluorone-Mo(VI) complex

75%

Du B. , Wu D. , Zhang H. , Li Y. , Wei Q. , Li Z.

Chemia Analityczna

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2005

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tom Vol. 50, No. 4

795-806

EN

Determination of protein - phenylfluorone (PF)-Mo(VI) complex using Rayleigh light scattering (RLS) method in the presence of the OP emulsifier microemulsion has been performed. At pH = 2.72 the RLS spectrum of PF-Mo(VI) complex is enhanced in the presence of proteins. Such a behaviour was utilised in a new method for the quantitative determination of proteins. An OP emulsifier microemulsion was used in this determination to increase the sensitivity. Four proteins, including bovine serum albumin (BSA), human serum albumin (HSA), Lysozyme (Lys), and λ-globulin (λ - G) have been determined. The dynamic range for BSA was 0-80 ng mL-1 and the corresponding detection limit equaled 1.57 ng mL-1. The method is characterised by high sensitivity and simplicity, and provides results of satisfactory stability. It can be used for the determination of residual protein in penicillin samples. The relative standard deviations for the investigated proteins were less than 4.48%. and the recoveries were in the range of 96.35%-100.9%.

PL

Przeprowadzono oznaczanie kompleksu białko-fenylofluoren (PF)-Mo(VI) stosując metodę rezonansowego rozproszenia światła (RLS) w obecności emulgatora OP. W obecności białek, przy pH 2,72 widmo RLS kompleksu PF-Mo(VI) ulega wzmocnieniu. Właściwość tę wykorzystano w nowej metodzie ilościowego oznaczania białek. Dodanie emulgatora OP znacznie poprawiało czułość. Oznaczono w ten sposób cztery białka: albuminę wolową (BSA). albuminę ludzką (HSA). lizozym (Lys) i λ λ-globulinę. Zakres dynamiczny BSA wynosił 0-80 ng mL-1, a granica wykrywalności - 1,57 ng mL-1. Metoda charakteryzuje się wysoką czułością! prostotą oraz dobrą powtarzalnością wyników. Względne odchylenie standardowe oznaczanych białek było niższe od 4,48% a odzyski zawierały się w granicach 96,35-100,9%.