Interleukin 2 (IL-2) is the fundamental growth factor for T lymphocytes, both in vivo and in vitro. At our department, specific mouse T lymphocyte clones are used for the examination of antigenic structure of Toxoplasma gondii. Their culturing in vitro requires big amounts of IL-2. That is why we determined optimal conditions for producing crude IL-2 preparation, using recombinant myeloma line X63/0 IL-2 as the producer of IL-2 and cytotoxic lymphocyte line CTL-L as the indicator of IL-2 activity.
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