Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of bovine PRNP gene polymorphisms on BSE susceptibility in cattle

100%

Gurgul A. , Slota E.

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tom 55

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nr 3-4

2

Adptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene

80%

Smolucha G. , Gurgul A. , Piestrzynska-Kajtoch A. , Rejduch B.

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tom 30

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nr 2

EN

The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this polymorphism was excluded from the assay. Such genotyping problems were not observed in earlier studies describing the method and cannot be easily explained. However, full accordance of genotypes in codons 136, 141 and 156 with sequencing results was obtained and thus the method can be recommended for routine application. Simultaneously, we strongly recommend to pay special attention when using the minisequencing method for genotyping codon 171 in sheep PRNP.

3

The effect of using DNA obtained from blood of cattle with genetic chimerism on illumina’s beadchip assay performance

80%

Gurgul A. , Rubis D. , Bugno-Poniewierska M.

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tom 14

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nr 2

4

Identification of carriers of the mutation causing coagulation factor XI deficiency in Polish Holstein-Friesian cattle

80%

Gurgul A. , Rubis D. , Slota E.

Journal of Applied Genetics

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2009

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tom 50

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nr 2

149-152

EN

Factor XI (FXI) deficiency is a hereditary coagulation disorder observed in various mammalian species. The molecular basis of coagulopathy has been recognized in Holstein cattle as a 76-bp insertion in the coding region of the FXI gene. Because the disorder seems to have an impact on reproductive traits and udder health in cattle, we tested 103 randomly selected cows, 28 cows with repeat breeding, and 9 cows with recurrent mastitis for the presence of an abnormal FXI allele. Three related cows were diagnosed as carriers.

5

PRNP and SPRN genes polymorphism in atypical bovine spongiform encephalopathy cases diagnosed in Polish cattle

80%

Gurgul A. , Polak M. P. , Larska M. , Slota E.

Journal of Applied Genetics

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2012

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tom 53

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nr 3

EN

Polymorphisms in the coding region of the prion protein gene (PRNP) have been associated with the susceptibility and incubation period of prion diseases in humans and sheep. However, polymorphisms in this part of the bovine PRNP gene do not affect the classical bovine spongiform encephalopathy (BSE) susceptibility in cattle. Studies carried out in Germany have shown that insertion/deletion-type polymorphisms located in the promoter region of the bovine prion gene are possible genetic factors modulating BSE susceptibility by changing the level of PRNP expression. No such association was observed for atypical BSE cases; however, due to the rare nature of the disease, these results should be confirmed. Additionally, a single nonsynonymous mutation in PRNP codon 211 (E211K) was described in one H-type BSE case in the USA; however, it was not found in any other cases. Here, we performed genetic characterization of PRNP promoter indel variations and determined the polymorphism of open reading frames (ORFs) of PRNP and bovine prion-like Shadoo (SPRN) genes in six Polish atypical BSE cases and compared these results to the population of clinically healthy Polish Holstein cattle. No potentially pathogenic mutations were found in the PRNP ORF in atypical BSE-affected cattle, but our study showed a high frequency of deletions at the indel loci of PRNP promoter in these animals. Additionally, a rare sequence variation in the SPRN protein-coding sequence was found in one L-type atypical BSE-affected animal.

6

The genetic basis of piebald coat colour in Hucul horses in the context of white markings and crypto-tobiano as a breeding problem in Poland

80%

Pasternak M. , Krupinski J. , Gurgul A. , Bugno-Poniewierska M.

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nr 4

7

Badania tramwajowego silnika trakcyjnego LTd-200

80%

Chądzel M. , Dudzikowski I. , Gurgul A.

TTS Technika Transportu Szynowego

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1998

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tom R. 5, nr 3

29-32

PL

W eksploatowanych silnikach tramwajowych typu LTd-220 stosowane jest przewietrzanie obce za pomocą wentylatora napędzanego z wirującej przetwornicy. Zespół: silnik - wentylator - przetwornica jest żródłem dużego hałasu, bardzo uciążliwego dla pasażerów oraz jednym z najbardziej zawodnych węzłów. Wysoka awaryjność przetwornic jest jedną z najczęściej występujących przyczyn awarii taboru. Przedmiotem pracy są badania eksploatacyjne i laboratoryjne silnika LTd-220 z przewietrzaniem obcym oraz analiza obliczeniowa analogicznego silnika z przewietrzaniem własnym.

8

Defekty genów u koni czystej krwi arabskiej - problemy hodowli

80%

Zabek T. , Bugno-Poniewierska M. , Gurgul A.

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2013

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tom 40

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nr 2

9

The application of genome-wide SNP genotyping methods in studies on livestock genomes

61%

Gurgul A. , Semik E. , Pawlina K. , Szmatola T. , Jasielczuk I. , Bugno-Poniewierska M.

Journal of Applied Genetics

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2014

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tom 55

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nr 2

10

Relevance of molecular changes in the ND4 gene in German Shepherd dog tumours

61%

Slaska B. , Grzybowska-Szatkowska L. , Bugno-Poniewierska M. , Gurgul A. , Smiech A. , Rozanska D. , Dudka J.

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nr 3

EN

The aim of the study was to identify polymorphisms and mutations in the mitochondrial ND4 gene and to analyse the associations between the occurrence of molecular changes in mtDNA and phenotypic traits in tumours in German Shepherd dogs. Fifty samples obtained from blood and tumour tissues of German Shepherd dogs with diagnosed tumours were analysed. DNA extraction, amplification, and sequencing of the mtDNA ND4 gene, and bioinformatics, statistical, and in silico protein coding SNP analyses were performed. ND4 mutations and/or polymorphisms were noted in eleven nucleotide positions in nearly half of the examined dogs. All the changes were substitution mutations. A majority of the changes identified were homoplasmic. In one dog with osteosarcoma, blood heteroplasmy was detected. In two positions of the ND4 gene, presence of non-synonymous mutations leading to amino acid changes in the ND4 protein was reported. Analyses carried out to determine the deleterious effect of mutations indicated an almost 97 and 62% probability that a single amino acid substitution (p.G239V and p.I401T, respectively) in the protein has a negative impact on its function. The results of statistical analyses indicate a significant association between the occurrence of mutations in three loci of the ND4 gene and the location of tumours. The mutations identified may be a result of cell adaptation to the changes in the environment occurring during carcinogenesis. The high frequency of mutations in the tumours may indicate genetic instability of mtDNA, which may also play a role in carcinogenesis.

11

Diversifying selection signatures among divergently selected subpopulations of Polish Red cattle

61%

Gurgul A. , Jasielczuk I. , Semik-Gurgul E. , Szmatola T. , Majewska A. , Sosin-Bzducha E. , Bugno-Poniewierska M.

Journal of Applied Genetics

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2019

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tom 60

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nr 1

12

Identification of mRNA degradome variation dependent on divergent muscle mass in different pig breeds

61%

Piorkowska K. L. , Szmatola T. , Pawlina-Tyszko K. , Gurgul A. , Zak G. , Ropka-Molik K. M.

Annals of Animal Science

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2020

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tom 20

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nr 4

13

Gene mapping as a method for verifying sequences localization based on interspecific chromosome painting (ZOO-FISH)

61%

Bugno-Poniewierska M. , Slota B. , Pawlina K. , Potocki L. , Gurgul A. , Slota E. , Klukowska-Rotzler J.

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2014

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tom 62

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nr 1

14

The use of runs of homozygosity for estimation of recent inbreeding in Holstein cattle

61%

Gurgul A. , Szmatola T. , Topolski P. , Jasielczuk I. , Zukowski K. , Bugno-Poniewierska M.

Journal of Applied Genetics

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2016

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tom 57

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nr 4

15

Profiles of miRNAs and their isomiRs in the rainbow trout (Oncorhynchus mykiss) eggs exposed to X-raysfor the androgenetic purpose

61%

Ocalewicz K. , Pawlina-Tyszko K. , Gurgul A. , Szmatola T. , Jasielczuk I. , Bugno-Poniewierska M. , Dobosz S.

Acta Biochimica Polonica

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2018

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tom 65

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nr S2

16

Epigenetic structure and the role of polymorphism in the shaping of DNA methylation patterns of equine OAS1 locus

61%

Zabek T. , Semik E. , Wnuk M. , Fornal A. , Gurgul A. , Bugno-Poniewierska M.

Journal of Applied Genetics

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2015

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tom 56

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nr 2

17

The evaluation of bovine SNP50 beadchip assay performance in Polish red cattle breed

61%

Gurgul A. , Zukowski K. , Pawlina K. , Zabek T. , Semik E. , Bugno-Poniewierska M.

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nr 3-4

18

Identification of a new haplotype within the promoter region of the MSTN gene in horses from five of the most common breeds in Poland

61%

Stefaniuk M. , Kaczor U. , Augustyn R. , Gurgul A. , Kulisa M. , Podstawski Z.

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2014

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tom 62

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nr 3

19

Characterisation of genome-wide structural aberrations in canine mammary tumours using single nucleotide polymorphism (SNP) genotyping assay

57%

Surdyka M. , Gurgul A. , Slaska B. , Pawlina K. , Szmatola T. , Bugno-Poniewierska M. , Smiech A. , Kasperek K. , Surdyka M. , Gurgul A. , Slaska B. , Pawlina K. , Szmatola T. , Bugno-Poniewierska M. , Smiech A. , Kasperek K.

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2019

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nr 1

20

General assessment of copy number variation in normal and tumor tissues of the domestic dog (Canis lupus familiaris)

51%

Gurgul A. , Zukowski K. , Slaska B. , Semik E. , Pawlina K. , Zabek T. , Jasielczuk I. , Bugno-Poniewierska M.

Journal of Applied Genetics

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2014

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tom 55

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nr 3