Wyniki wyszukiwania - Biblioteka Nauki

1

Stosunek Polaków do pracy a „zrównoważona” polityka zatrudnienia w Polsce w kontekście strategii europejskich

100%

Stasiak M.

Świat Idei i Polityki

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2013

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nr 12

217-235

EN

The international documents, especially Lisbon Strategy and Europe 2020 strategy, oblige Polish government to the implementation of the principles of the sustainable development in the employment policy. Analysis of Polish and European employment strategies are pointing that priority like: just, free from poverty, equal opportunities for women, men and young people on the labour market, economic activity of older people, protection of the environment or harmfulness of the work to the environment, proving that they are not effectively carried out in Poland. Employment policy is also ineffective, because the main strategies are not taking into account attitudes of Poles to the work, and values which are shaping this attitude. Return to values is extremely important for the implementation of sustainable development, because for this idea, values shape human and social reality, thus work reality.

2

O banalności dobra wspólnego – studium hermeneutyczne

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Stasiak M.

Świat Idei i Polityki

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2015

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nr 14

9-24

PL

Artykuł – zbudowany z trzech sekwencji tematycznych – stanowi próbę wypracowania nowego wymiaru idei dobra wspólnego, możliwego do urzeczywistnienia w wymiarze praktycznym, szczególnie w perspektywie uszczuplających się zasobów naturalnych. Po pierwsze, autorka definiuje pojęcia takie jak: dobro, wspólnota, dobro wspólne, wskazując tendencje do dwubiegunowego myślenia o powyższych ideach: relatywistycznego i absolutystycznego. Drugim etapem rozważań jest analiza idei z perspektywy hermeneutyki. Autorka uzasadnia tytułową banalność dobra wspólnego, tkwiącą nie w samej idei, ale w sposobie myślenia o niej. Trzecia sekwencja tematyczna stanowi próbę wyjaśnienia nowego podejścia do zagadnienia dobra wspólnego, którego najważniejszym elementem jest jego podział na dobra formalne oraz materialne (definiowane odtąd na gruncie polityki nie zaś etyki).

EN

Article is an attempt for the creation a new way of thinking about the common good, which is possible to realize in practice (particularly in view of depleting natural resources). Firstly, author defines terms such as: the good, the community , the common good, indicating a tendency to bipolar thinking about these ideas: relativist and absolutist. The second part consist analysis of these ideas from the one perspective – hermeneutics. In this part „banality” is not a „name” for common good. It is a „name” for the way of thinking (bipolar). In the third thematic sequence, author explain the new way of thinking. From now we have formal and material good defined in political sphere rather than ethics.

3

GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

51%

Czyż M. , Stasiak M. , Boncela J. , Cierniewski C. S.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

19-28

EN

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αV promoter region and are directly involved in the regulation of transcriptional activity of the αV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αV expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the αV gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αV mRNA and αV protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αV. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αV gene promoter and negatively regulates αV gene expression.

4

Combined effects of doxorubicin and STI571 on growth, differentiation and apoptosis of CML cell line K562

45%

Jakubowska J. , Stasiak M. , Szulawska A. , Bednarek A. , Czyz M.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

839-846

EN

STI571 (imatinib mesylate; Gleevec®) is an inhibitor that targets the tyrosine kinase activity of Bcr-Abl present in chronic myelogenous leukemia (CML) cells. Some preclinical studies have demonstrated that the combination of STI571 with chemotherapeutic drugs results in enhanced toxicity in Bcr-Abl-positive leukemias. We investigated the potential benefit of using STI571 to down-regulate Bcr-Abl activity for the enhancement of doxorubicin anti-proliferative action in K562 cell line derived from blast crisis of CML. At low concentrations of both drugs (40 nM doxorubicin combined with STI571 in the range of 100-150 nM), the antiproliferative effects were mainly due to cellular differentiation as assessed by benzidine staining for hemoglobin synthesis level and real-time PCR for γ-globin expression. Higher concentrations of STI571 used in combinations with doxorubicin caused mainly apoptosis as shown by DNA degradation and nuclear fragmentation visualized by fluorescence microscopy after DAPI staining, changes in cell morphology observed after Giemza-May Grünwald staining and cellular membrane organization estimated by flow cytometry after Annexin V staining. As compared with either drug alone, cotreatment with STI571 and DOX induced stronger cellular responses. A low concentration of STI571 in combination with a low concentration of DOX might be tested as an alternative approach to increasing the efficacy of chemotherapy against CML.