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EN
The objective of the study was to establish a percent of infections caused by equine arteritis virus (EAV) in chosen studs of breeding horses in Poland. A total of 711 samples of sera of clinically normal mares and stallions were examined in 1989—1990. Six hundred were obtained from arabian horses (302 from the stud J, 74 K and 224 M) and 111 samples were obtained from the stud S of thoroughbred horses. Serological examinations showed the presence of infection caused by EAV. In the stud J 5.3%, K 6.8%, M 48.7% and S 72.1% of sera reacted positively.
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nr 10
482-483
EN
Attempts of using stallions naturally infected with Equine arteritis virus in reproduction The objective of the studies was to check both in vitro and vivo the use in reproduction of convalescent stallions of semen free from the Equine arteritis virus (EAV). The examinations were done on 92 stallions from the stud К in which sera of 83.2% of animals were free from antibodies to EAV. Three stallions used for mating in 1993 were seropositive. Attempts to isolate the EAV from semen were performed on RK-13 and Vero cell cultures. From the semen of one stallion a virus identified as AEV by the direct immunofluorescence test was isolated. As for the two remaining convalescent stallions with negative results of the laboratory test for EAV, semen was examined for the EAV transfer on four fully susceptible mares.
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tom 47
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nr 10
EN
The purpose of the work was to determine the percentage of infections with arteritis virus and the analysis of epizootiological state in the stud of horses in the Upper Silesia. In the first period of studies the percentage of mares with specific antibodies was 58.3 per cent and after 6 months it increased by 79 per cent. It was lowest in young mares aged 3—4 years. The SN titres against EAV ranged between 4—512. In most cases they fluctuated between 32—256. The titre in one stallion was 128—256 while in another one no antibodies were found at all. Sera of mares without antibodies at day of mating showed the presence of the antibodies after three weeks following mating by a stallion serologically positive; that was not observed in case of mating by a stallion serologically negative. The findings indicated that in the horses under study equine arteritis virus was transmitted through the genital system during mating and the stallion’s semen was the source of infection.
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tom 54
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nr 09
626-627
EN
In the study flow cytometry was attempted to identify EAV infection in stallions’ semen. Material for the study consisted of 8 semen samples taken from EAV-seropositive stallions. The samples were put on RK-13 and Vero cells. After a 24-hour incubation period the cells were treated with a specific conjugate and then analyzed in FACS aparatus. In the case of two samples positive fluorescence was observed. The above positive result was confirmed by virus isolation in both cell cultures used.
EN
Intervention vaccinations were undertaken after 2.5 weeks since the first cases of abortions, i.e. when the percentage of abortions was 23. There were used the following vaccines: Equivac RP, Prevaccinol and RPK. Immunizations were performed on 3 groups of mares: group A comprised 19 animals vaccinated once with Equivac at months 8—9 or 9—10 of pragnancy. Group В containing 29 pregnant mares was immunized 3 times: at first (9 animals) in the 2—3 month, 10 animals in the 4—5th month and 9 animals in the 5—6th month, then for the second time in the months 4—5, 6—7 and 7—8, and for the third time in months 6—7, 8—9 and 9—10. The third group (C) was immunized for the first time when the mares were not pregnant, then in the 3—4 and 7—8th month of pregnancy. The percentage of abortion was 94.7% (group A), 10.3% (group B) and 0% (group C). Only three fold vaccinations protected mares from abortions in the infected environment.
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