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Interleukin-6 biology is coordinated by membrane bound and soluble receptors

100%

Rose-John S.

tom 50

nr 3

Cytokine receptors exist in membrane bound and soluble form. Both forms bind their ligands with comparable affinity. While most soluble receptors are antagonists in that they compete for the ligands with their membrane counterparts, some soluble receptors are agonists. In this case, the complex of ligand and soluble receptor binds on target cells to a second receptor subunit and initiates signal transduction. Soluble receptors of the IL-6 family of cytokines are agonists. In vivo, the IL-6/soluble IL-6R complex stimulates several types of target cells not stimulated by IL-6 alone, since they do not express the membrane bound IL-6R. This process has been named transsignaling. We have shown that in several chronic inflammatory diseases like chronic inflammatory bowl disease, peritonitis and rheumatoid arthritis, transsignaling via the soluble IL-6R complexed to IL-6 is a crucial point in the transition from the acute to the chronic state of the disease. The mechanism by which the IL-6/ soluble IL-6R complex regulates the inflammatory state is discussed.

Interferon-gamma regulates alpha-2-macroglobulin and alpha-1-antichymotrypsin expression on the pretranslational level in HepG2 cells

51%

Kordula T. , Rose-John S. , Heinrich P. C. , Koj A.

nr 4

Biosythesis and distibution of leucocyte elastase inhibitor. Production of recombinant in inhibitor

51%

Kasza A. , Korpula-Mastalerz R. , Rose-John S. , Dubin A.

nr 3

The horse leucocyte elastase inhibitor (HLEI), present in neutrophils, monecytes and bone marrow cells, is apparently a cytoplasmic protein which is not released from cells even in response to stimulation with lipopolysaccharide, phorbol ester, tumour necrosis factor alpha, interleukin-1 or elastin degradation products. Although no expression of the inhibitor was detected in neutrophils, both monocytes and bone marrow cells were efficient in its synthesis. Using a new expression vector pREST5d, recombinant inhibitor was produced in a large quantity in a soluble form, with a yield of 88 mg per 10 litres of E. colt culture. A two-step purification procedure, consisting of ion-exchange chromatography and gel filtration, yielded 36 mg of the recombinant inhibitor of a purity higher than 95%, as judged by SDS/PAGE. The recombinant protein had physicochemical and kinetic properties indistinguishable from those of the natural one, including irreversible elastase inhibition with an association rate constant kass > 10 7 M -1 s -1. Both proteins were eliminated from rat circulation at the same ratio, and within the first 20 min 70% of the protein was removed. Such a short half-life in the circulation suggests that local delivery of HLEI directly to lungs in the form of aerosol could be a more efficient therapeutic approach than its intravenous injection.

Human melanoma gene therapy: from animal studies to clinical trials

26%

Mackiewicz A. , Kapcinska M. , Laciak M. , Malicki J. , Murawa P. , Nowak J. , Sibilska E. , Wiznerowicz M. , Breborowicz D. , Lange A. , Hawley R. G. , Heinrich P. C. , Rose-John S.

nr 4