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2017
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tom Vol. 18, nr 4
142--147
PL
Prawidłowa eksploatacja oczyszczalni wymaga monitorowania parametrów procesu oczyszczania, jak i efektywności poszczególnych jego etapów. Wymagania prawne dotyczą nie tylko związków organicznych (BZT5, ChZT) i zawiesin ogólnych, ale również wysokoefektywnego usuwania związków biogennych: azotu i fosforu. Efektywność usuwania związków biogennych zakłóca m.in wahania temperatury, jakość ścieków, problemy z działaniem osadu czynnego. Celem badań było określenie zmian zawartości związków organicznych, związków azotu i fosforu w poszczególnych etapach mechaniczno-biologicznego oczyszczalnia ścieków. Próbki pobierano zgodnie z przepływem wzdłuż przekroju oczyszczalni: ścieki surowe, po mechanicznym oczyszczeniu, po komorze predenitryfikacji, defosfatcji, denitryfikacji, ścieki oczyszczone. Innym aspektem prowadzonych badań było wyznaczenie frakcji ChZT oraz ich zmian w ściekach komunalnych, po kolejnych etapach mechaniczno-biologicznego oczyszczania. Frakcjonowanie umożliwia wyodrębnienie rozpuszczonych i nierozpuszczalnych substancji organicznych, uwzględniając dodatkowo ich biodegradowalność, a także brak podatności na biologiczny rozkład, co stanowić bardzo istotny element kontroli procesów zachodzących podczas oczyszczania ścieków.
EN
Proper exploitation of waste water treatment plant is strictly connected with monitoring of basic parameters and effectiveness of particular its stages. Legal requirements include not only organic compounds (BOD5, COD) and general suspensions but also highly effective removal of nutrients: nitrogen and phosphorus. The effectiveness of biogenic compounds removal is hindered by temperature fluctuations, effluent quality, and problems of active sediment. The aim of this study was to show the changes in concentrations of organic compounds, nitrogen and phosphorus in the municipal wastewater after subsequent stages of mechanical-biological treatment. During the research, samples were collected during consecutive stages of wastewater treatment: raw wastewater, wastewater after mechanical treatment, pre-denitrification, dephosphatation, denitrification, nitrification and treated wastewater. Determination of COD fractions, and their changes in the municipal wastewater, after the successive stages of mechanical-biological treatment constituted another aspect of this study. It allows separation of dissolved and non-dissolved organic substances, also taking into account their biodegradability and the lack of susceptibility to biological decomposition. This method can also be very important for the control of processes during wastewater treatment.
EN
On the basis of EC6 medium, specific conditions were established to improve its properties as a nutrient medium for isolated, immature zygotic embryos of white clover. The highest frequency of embryo development occurred on medium with 117 mM sucrose concentration and 30% (v/v) coconut water for globular-stage (23.3%) and heart-stage embryos (78.3%). The development of globular-stage embryos was abnormal, however. The embryos callused after two weeks of culture. From the callus produced, plantlets and then plants were regenerated. A double-layer culture system, with the top layer having higher osmolarity than the bottom layer, enabled proembryos smaller than 60 µm to be cultured. For in ovulo embryo culture, Nitsch medium supplemented with 10% (v/v) cucumber juice proved most suitable. On this medium, approximately 13% of the ovules containing few-celled embryos germinated and produced seedlings.
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nr 2
EN
In vitro somatic embryogenesis and organogenesis (shoot and root formation) were observed when immature zygotic embryos of the sunflower cultivars Frankasol, Printasol, Lech and Wielkopolski were cultured on JME medium containing high sucrose concentration (350 mM). Of four tested cultivars, Frankasol showed the highest rate of somatic embryogenesis, and Printasol the highest rate of organogenesis. The remaining two cultivars responded slightly.
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tom 42
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nr 1
EN
The effect of 1%, 3% and 10% fructose, glucose, sucrose and ribose on callus induction and organogenesis was studied in Brassica napus L. cv. Evita. Hypocotyls and cotyledons of 7-day-old seedlings were used as explants. MS (Murashige and Skoog, 1962) was the basal medium. Calluses were produced from both types of explants in the presence of 2,4-D. There were significant differences in the frequency of callus induction between ribose and other sugars tested, as well as between low (1% and 3%) and high (10%) sugar concentrations. Irrespective of sugar type, callus induction was significantly lower on ribose- containing media and at high concentration. On hormone-free media, callus tissue formed very exceptionally and only from cotyledons. The amounts of callus tissue produced were highest on MS with glucose, followed by sucrose and fructose. In the regeneration experiments, explants were cultured on MS with 2,4-D as the sole growth regulator, and with NAA and kinetin. No regeneration occurred on medium with 2,4-D. In the presence of NAA and kinetin, organogenesis was observed only on media with 1% and 3% sugars, but on ribose the number of organs produced was very low. The highest regeneration ability occurred on sucrose-based medium.
EN
Callus was successfully induced from the mature endosperm of three Actinidia species: A. arguta, A. deliciosa var. deliciosa (kiwifruit) and A. kolomikta. For the initiation of callus, MS medium supplemented with 2,4-D (2 or 4 mg/l) and kinetin (5 mg/l) was used. Transfer of the callus to medium containing IAA (0.1 mg/l) and BAP (1 mg/l) resulted in the formation of roots in approximately 40% of the endosperm explants of A. deliciosa. The callus, initially yellow-white, turned green when cultured in the light. In A. arguta and A. kolomikta no morphogenic response was observed on this medium. If the cultures were inoculated onto medium with IAA (0.3 mg/l) and 2iP (5 mg/l), in the endosperm calluses of kiwifruit, shoots were formed in addition to roots. In A. arguta a few abnormal shoots were produced in one explant. The sub-culture of A. arguta callus on MS without hormone evoked the production of some roots. No morphogenic response was observed in the endosperm cultures of A. kolomikta on all media tested. The counting of chromosomes in five roots and young leaves of one shoot of A. deliciosa revealed that they were triploids with chromosome number 2n = ~250.
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tom 39
EN
Patterns were examined in published karyological data for 1451 moss species (approximately 16% of the total number of species) from all regions of the world. Assuming that x = 7 is the original basic chromosome number in mosses, all gametophytic numbers n > 9 were supposed to mark polyploids. It was found that 84% of the species had undergone polyploidy in their history. It is assumed that most polyploids in mosses are autopolyploids. Among the polyploids, 28% are high polyploids (species with n > 15). In pleurocarpous mosses, 16% are high polyploids, whereas in acrocarpous mosses 27% are high polyploids. There is no difference in frequencies of high polyploids between the two subgroups of acrocarpous mosses: Haplolepideae-acrocarpae (26%) and Diplolepideae- acrocarpae (27%). A clear correlation was found between the frequency of high polyploids (n > 15) and latitude. The frequency of high polyploids increases from the equator towards the North and South Poles. The percentage of high polyploids jumps dramatically from tropical and subtropical zones (- 13%) to temperate and cold climatic zones (over 30%).
EN
It is particularly difficult to treat wastewater from the production of edible oils because of high concentration of phosphates, sulphates as well as organic compounds. What is more, the application of physicochemical methods of purification may be limited due to the varied wastewater character and its chemical properties. Phosphates precipitated by means of calcium hydroxide turn into calcium phosphates, usually in the form of hydroxyapatite (HAP), which together with an excessive amount of calcium hydroxide create well-sedimenting sludge. However, the industrial application of the method for the treatment of the analyzed effluent leads to the burdensome floating sludge. The research project was carried out to establish the causes of partial sludge floating. Raw and treated wastewater (after sedimentation process) as well as sludge samples collected in the settling tank were analyzed with a view to identify their chemical content. Furthermore, the sludge samples were mixed mechanically and stored in anaerobic conditions and the sedimentation process was studied in laboratory conditions. The research focused on basic physicochemical parameters, including concentration of calcium and fatty substances. It was established that the pretreatment process may be hindered by an excessive amount of fatty substances, which generate lime-fatty complex compounds. Moreover, calcium hydroxide used for phosphates precipitation lead to the release of sulphates into the treated wastewater.
PL
Ścieki pochodzące z produkcji olejów jadalnych są trudne do oczyszczenia ze względu na duże stężenia fosforanów, siarczanów i związków organicznych. Ze względu na zróżnicowany charakter i skład ścieków przemysłowych stosowanie fizykochemicznych metod usuwania zanieczyszczeń może być związane z pewnymi ograniczeniami. Podczas strącania fosforanów za pomocą wapna powstają fosforany wapnia zazwyczaj w postaci hydrosyapatytu (HAP), który wraz z nadmiarem wodorotlenku wapnia tworzy dobrze sedymentujący osad. Zastosowanie tej metody do oczyszczania badanych ścieków w warunkach technicznych powoduje powstawanie uciążliwego osadu flotującego na powierzchni ścieków. Szukając przyczyn wypływania części osadów, wykonano badania obejmujące charakterystykę jakościową ścieków dopływających oraz po procesie sedymentacji, a także osadów zatrzymywanych w osadniku. Zmierzając do lepszego poznania procesów zachodzących w osadniku, badano wpływ mieszania mechanicznego na osady przetrzymywane w warunkach laboratoryjnych. Analizowano podstawowe parametry fizykochemiczne, w tym zawartość wapna oraz substancji tłuszczowej. Stwierdzono, że obecność w ściekach tłuszczy w znacznych stężeniach przyczynia się do powstawania związków wapniowo-tłuszczowych flotujących na powierzchni ścieków, co w rezultacie zakłóca proces podczyszczania ścieków. Ponadto wapno stosowane do strącania fosforanów przyczynia się do uwalniania jonów siarczanowych.
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