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Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

100%

Nadas G. S. , Kalmar Z. , Taulescu M. A. , Chirila F. , Bouari C. M. , Rapuntean S. , Bolfa P. , Iosif Fit N.

nr 2

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata -10(17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.

Efficacy of natural formulations in bovine mastitis pathology: alternative solution to antibiotic treatment

100%

Pasca C. , Marghitas L. A. , Dezmirean D. S. , Matei I. A. , Bonta V. , Pasca I. , Chirila F. , Cimpean A. , Fit N. I.

Journal of Veterinary Research

2020

tom 64

nr 4