Wyniki wyszukiwania - Biblioteka Nauki

1

Tramobus w Clermont-Ferrand we Francji

100%

Piliszek A.

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tom nr 4

7-11

PL

W artykule przedstawiono wdrożony we Francji system tramwaju Translohr poruszającego się na kołach samochodowych nazywany tramobusem. Rys historyczny projektu, projekt nowej linii tramwajowej, infrastruktura tramwajowa, zajezdnia i centrum sterowania. Tramobus Translohr na świecie.

EN

There is presented in the article the tramway system Translohr implemented in France that is on the tyres and is called tram-bus. The historical draft of the project, the project of a new tramway line, the tramway infrastructure, the tram depot and guidance system. The tram-bus Translohr in the world.

2

Chimerowe zarodki ssakow: powstawanie i wykorzystanie

100%

Piliszek A.

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2004

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nr 1

142-155

3

W poszukiwaniu idealnego biomarkera obrazowego dla wczesnego udaru mózgu : obrazowanie tensora dyfuzji MR (DTI)

51%

Walecki J. , Szary C. , Piliszek A.

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tom Vol. 3, nr 2

67--69

PL

Rezonans magnetyczny (RM) z obrazowaniem dyfuzyjnym (DWI) jest badaniem o bardzo wysokiej czułości i swoistości w rozpoznawaniu wczesnych zmian niedokrwiennych/obrazowaniu obrzęku cytotoksycznego. W ostatnich kilku latach wzrosło zainteresowanie wykorzystaniem tensora dyfuzji do oceny dynamiki wczesnych zmian w przebiegu udaru niedokrwiennego mózgu.

4

Potencje rozwojowe komórek somatycznych ssaków

51%

Modlinski J. A. , Piliszek A. , Karasiewicz J.

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nr 1

5

From blastomeres to somatic cells: reflections on cell developmental potential in light of chimaera studies - a review

51%

Zyzynska-Galenska K. , Piliszek A. , Modlinski J. A.

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nr 3

EN

Mammalian development is a process, whereby cells from a totipotent zygote gradually lose their potency, i.e. their ability to differentiate, in the environment of the developing embryo. An ideal model for testing the real potential of cells is the experimental production of chimaeras. The first experimentally produced mammalian (murine) chimaeras were created by Tarkowski [1961] and since then many new methods of chimaera production have been developed, including injecting cells into the blastocyst’s cavity or into cleaving embryos. This review describes how different cell types, depending on the developmental stage or culture conditions, manifest their potential to contribute to chimaeras. Cell developmental potential has been analysed in pluripotent blastomeres, which can contribute to all embryonic and extra-embryonic lineages, albeit differently depending on their developmental stage. This is the case in blastocyst lineages, with various developmental potentials depending on the surrounding cells, and in more differentiated cells from different stages of pregnancy, which in some cases may colonise chimaeric animal tissue. Cell potential has also been analysed in embryonic stem and embryonal carcinoma cells, which are pluripotent and efficiently contribute to chimaeras; in multipotent fetal and adult stem cells, which can also participate in chimaera formation; and in somatic mouse embryonic fibroblasts (MEFs), which can also be reprogrammed in the environment of the cleaving embryo. Interspecies chimaera studies have also demonstrated the pluripotency of foreign cells. Experiments with chimaeras have shown that not only pluripotent embryonic cells are capable of contributing to chimaeras, so are adult cells, which plasticity is now well-documented.

6

Obtaining farm animal embryos in vitro

45%

Duszewska A. M. , Rapala L. , Trzeciak P. , Dabrowski S. , Piliszek A.

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nr 2

EN

This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

7

Uzyskanie cielat po transferze zarodkow, do ktorych wprowadzono konstrukcje genowa

32%

Duszewska A. M. , Wojdan J. , Gawron W. , Lipinski D. , Piliszek A. , Wenta-Muchalska E. , Was B. , Slomski R. , Reklewski Z.

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nr 12

1323-1325

8

Cattle offspring obtained after transfer of IVP, GFP positive and GFP positive-frozen/thawed embryos

32%

Duszewska A. M. , Lipinski D. , Piliszek A. , Slomski R. , Gawron W. , Wenta-Muchalska E. , Was B. , Chromik A. , Reklewski Z.

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tom 22

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nr 4

9

Controversial aspect of using GFP as a marker for the production of transgenic cattle

26%

Duszewska A. M. , Lipinski D. , Piliszek A. , Slomski R. , Plawski A. , Wojdan J. , Gawron W. , Juzwa W. , Zeyland J. , Wenta-Muchalska E. , Reklewski Z.

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nr 4

EN

The objective of this study was to examine the feasibility of identification and selection of cattle embryos based on green fluorescence (GFP-positive) in order to obtain calves carrying an integrated transgene. The construct used (pbLGTNF-EGFP) contained the human tumor necrosis factor alpha (hTNFa) gene fused to the bovine betalactoglobulin promoter (bLG) in plasm id vector pCX-EGFP. In four experiments, 76 zygotes were injected; eight of them developed to the morulac/blastocysts stage of which only five were GFP positive (one of them 100%, one-50%, three- 25%). All of the GFP positive embryos were transferred to recipients. Two calves were born: one after transfer of the 100% GFP positive embryo and the other after transfer of one of the 25% GFP positive embryos. Both animals were healthy with normal weight when compared to two control calves. The integration of pbLGTNF-EGFP in the host genome could not be detected in either of the calves, suggesting that GFP is an unreliable marker for preimplantation screening of embryos.