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A current view on drug sensitivity of Neisseria gonorrhoeae isolated during the infection in men

100%

Bialecka J. , Laskowska M. , Bacula-Sladowska K. , Kasprowicz A.

Postępy Mikrobiologii. Suplement

2017

tom 56

nr 3

The occurrence and comparative phenotypic characteristics of Staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods

63%

Kasprowicz A. , Bialecka A. , Bialecka J. , Godzisz I. , Barabasz W. , Jaworska O. , Malachowa N. , Miedzobrodzki J.

nr 1

To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. A total number of 104 isolates representing different staphylococcal species were isolated and identified using routine biochemical methods applied in diagnostic laboratories. Among 17 isolated staphylococcal species, Staphylococcus intermedius was the most common species isolated from both healthy or diseased dogs living either in animal shelter or household environments. The pattern of Staphylococcus sp. colonization differs considerably for animals living in the two tested habitats. In particular, S. aureus MRSA and MSSA isolates were detected only in infected skin lesion samples from animals that dwelled in the animal shelter. As could be expected, S. intermedius was found to be a predominant causative agent in canine skin infections. In our study, we demonstrated that S. intermedius in its carrier-state, inhabits mainly the mucosal membrane of the nasal vestibule. It was also found in the samples taken from the skin, the lumbo-sacralis triangle and perineum, but was rarely isolated from the ears.

New insight into genotypic and phenotypic relatedness of Staphylococcus aureus strains from human infections or animal reservoirs

63%

Lisowska-Lysiak K. , Kosecka-Strojek M. , Bialecka J. , Kasprowicz A. , Garbacz K. , Piechowicz L. , Kmet V. , Savini V. , Miedzobrodzki J.

tom 68

nr 1

Staphylococcus aureus is a common human and livestock opportunistic pathogen, and there is evidence of animal to human transmission.This paper aimed to recognize properties of the isolates from collections of human and livestock S. aureus strains and to estimate compat-ibility of results based on phenotypic tests, microarrays and the spa typing methods. The second goal was to study differences between human and animal isolates in terms of specificity of their hosts and the strain transmission among various hosts. Most strains showed multi-susceptible profiles and produced enzymes on a high level, and they were phenotypically and genetically similar. However, in contrast to the Polish bovine mastitis strains, the Slovakian strains were multi-resistant. In this research, the strains showed significant differences in terms of their phenotypic manifestations and the presence of hemolysins genes; however, other enzyme-encoding genes correlated to a higher extent with the microarrays results. Interestingly, there was a lack of enterotoxin genes in human Poultry-like protein A+ strains in comparison to other human strains. Our study showed that differences between virulence profiles of the human and animal strains cor-related with their origin rather than their hosts, and any trait allowed clearly distinguishing between them based on the microarray results.