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1 Journal of Applied Genetics

1 2009

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Detecting a deletion in the coding region of the bovine bone morphogenetic protein 15 gene [BMP15]

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Zhang L.-P. , Gan Q.-F. , Zhang X.-H. , Li H.-D. , Hou G.-Y. , Li J.-Y. , Gao X. , Ren H.-Y. , Chen J.-B. , Xu S.-Z.

Journal of Applied Genetics

2009

tom 50

nr 2

145-148

The aim of this study was to detect polymorphism in the bovine bone morphogenetic protein 15 (BMP15) gene. On the basis of PCR-SSCP and DNA sequencing, a 4-bp deletion was identified in the coding region of the gene. Sequence analysis revealed that the deletion altered the reading frame and introduced a stop codon at position 264. Eight breeds (Luxi, Qinchuan, Nanyang, Jinnan, Bohai Black, Menggolian, Holstein, and Simmental) were genotyped by PCR-SSCP. No cows homozygous for this mutation were observed in these breeds. Heterozygous cows were detected in Luxi, Qinchuan, Nanyang, Jinnan and Bohai Black cattle. Fecundity was not increased in heterozygous individuals.