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1 Medycyna Weterynaryjna

1 2006

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Polimorfizm genu PRNP w kodonach 136, 154 i 171 u polskich ras owiec

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Niznikowski R. , Luhken G. , Strzelec E. , Lipsky S. , Popielarczyk D. , Erhardt G. , Ajmone-Marsan P. , Bruford M. , Dunner S. , Obexer-Ruff G. , Taberlet P. , Valentini A. , Caloz R. , Georgudis A. , Canali G. , Roosen J. , Crepaldi P. , Togan I. , Vlaic A. , Fesus L. , Ertugrul O. , Abo-Shekada M. , El Barody M. , Hoda A. , Trommetter M.

Medycyna Weterynaryjna

2006

tom 62

nr 08

938-941

The study was conducted on six Polish sheep breeds (Polish merino, Zelaznenska sheep, Pomeranian, Kamieniecka sheep, Polish mountain sheep and Polish heath sheep). Individual blood samples were collected and DNA was extracted. The frequencies of alleles (ARR, ARH, ARQ, AHQ, VRQ) and its genotypes of PRNP gene were determined according to the RFLP-PCR method. Depending on the breed and sex, a high diversity of polymorphous forms of the PRNP gene was observed. Higher PRNP gene polymorphism was observed in ewes than in rams. The lowest frequency of alleles was determined in three breeds: Polish merino (ARR, ARQ and VRQ), Polish mountain sheep and Polish heath sheep (ARR, ARQ and AHQ). All five of the determined alleles were observed in Pomeranian and Kamieniecka sheep. The most advantageous frequencies of ARR allele occurred in Polish merino, Kamieniecka and Zelaznenska sheep (particularly in ewes). In Polish mountain sheep and Polish heath sheep the VRQ allele was not observed. In the final results, 11 combinations of genotypes occurred in six Polish sheep breeds. The obtained results indicate a need for the construction of an appropriate breeding program for each breed separately. These breeding programs should enable obtaining higher frequencies of ARR allele occurrence and restricting the incidence of other alleles of the PRNP gene, particularly VRQ allele.