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1

Evaluation of oxidative status in dogs with anemia

100%

Gultekin M. , Voyvoda H.

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2017

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tom 73

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nr 08

EN

The aim of the present study was to evaluate the relationship between the oxidative status and the severity and type of anemia in dogs. A total of 70 dogs of various breeds, ages and of both sexes were enrolled in the study. Fifty dogs with anemia were classified according to the severity of anemia as mildly (n = 18), moderately (n = 18) or severely (n = 14) anemic on the basis of the hematocrit (HCT) value. Anemia in the same dogs was also classified according to the type as regenerative (n = 26) or non-regenerative (n = 24) on the basis of the absolute reticulocyte count. Twenty dogs were used as healthy control. Total oxidant status (TOS), total antioxidant capacity (TAC) and malondialdehyde (MDA) values in plasma as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in erythrocyte hemolysate were measured to evaluate the oxidative status. The plasma TOS value was increased in all anemic dogs, irrespective of the severity of anemia, whereas a significant increase in the TAC value and a significant decrease in erythrocyte GSH-Px activity were observed in mild and moderate anemia. Plasma TOS and TAC values were higher in dogs with both types of anemia when compared to the healthy group. A significant increase in plasma MDA concentration and a significant decrease in GSH-Px activity were observed in the non-regenerative anemia group. There were moderate negative correlations between HCT and TOS values in the mild anemia group and between HCT and erythrocyte GSH-Px activity in the regenerative anemia group. In conclusion, oxidative stress develops in dogs with anemia, and it is largely independent of the severity and type of anemia. These results suggest that further studies with different etiologies may also be useful for evaluating the efficacy of antioxidants administered at different doses and in different combinations to treat anemia in dogs.

2

Oxidative status and lipid profile in mono- and co-infection with canine monocytic ehrlichiosis

70%

Gultekin M. , Ural K. , Pasa S. , Balikci C. , Asici G. S. E.

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nr 12

EN

Vector-borne diseases in dogs are a major health problem of zoonotic importance. Canine monocytic ehrlichiosis (CME) is one of the most common vector-borne infections in dogs. Co-infections with vectorborne diseases are reported to cause more severe pathological effects than mono-infections. The current study investigates the effects of mono- and co-infections with CME on oxidative status and lipid profiles in dogs. Dogs with vector-borne diseases were divided into two groups, mono-infected (n = 22) and co-infected (n = 23) with CME, and compared to a healthy group (n = 20). Double and triple co-infections with other vector-borne diseases included A. phagocytophilum, L. infantum, and D. immitis. The serum lipid profile (high-density lipoprotein (HDL), low-density lipoprotein (LDL), cholesterol, and triglycerides) and the total and individual markers of oxidative status (total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA), paraoxonase (PON-1), and arylesterase (ARE)) were evaluated. Significant decreases in PON-1 and ARE enzyme activities and HDL concentration, as well as increases in TOS level, MDA, LDL, and triglyceride concentrations were determined in both mono- and co-infected groups compared to the healthy control group. No significant differences between the groups were found in the TAC level or cholesterol concentration. In conclusion, alterations in lipid profile and increase in oxidative stress were observed in dogs with CME compared to the healthy control group, however, no significant differences were detected between mono- and co-infected dogs.

3

Chloroquine treatment against naturally occuring Giardia duodenalis infection in dogs

70%

Ural K. , Gultekin M. , Pasa S. , Aysul N. , Ayan A.

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2017

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tom 73

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nr 05

EN

The aim of the present study was to evaluate the efficacy of chloroquine on lessening or eliminating cyst shedding in dogs naturally infected with Giardia duodenalis. A total of 26 dogs naturally infected with G. duodenalis, from various breeds, ages and of both sexes were selected and randomly assigned into two groups based on treatment (group I, n = 20 dogs treated orally with 2.5 mg/kg chloroquine twice daily for 5 consecutive days) and control (group II, n = 6 untreated control dogs). Diagnosis was based on microscopic faecal examination and rapid test kits. Cyst excretion was determined on days 0, 3, 7 and 10, before and after treatment. Evaluation of the percentage reduction in cyst excretion revealed 99.8% efficacy on day 3 and 99.9% on days 7 and 10 in the treatment group. Geometric means of the number of excreted cysts did not change significantly in the control group. Chloroquine at the proposed dosage might be a practical in application, low priced and highly effective treatment option in dogs with giardiasis.

4

Interpretation of 25-OH-D3, NEFA, and calcium correlations among cow and calf pairs

61%

Erdogan S. , Ural K. , Erdogan H. , Ural D. A. , Gultekin M. , Pasa S.

Medycyna Weterynaryjna

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2021

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tom 77

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nr 04