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CamCon - projekt ograniczenia występowania bakterii Campylobacter w stadach brojlerów jako element zwalczania kampylobakteriozy

100%

Kania I. , Kusyk P. , Wieczorek K. , Osek J.

2012

tom 19

nr 01

Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

88%

Stojecki K. , Sroka J. , Karamon J. , Kusyk P. , Cencek T.

nr 1

Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

88%

Stojecki K. , Sroka J. , Karamon J. , Kusyk P. , Cencek T.

nr 1

Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and the effect of each component of pre-treatment solutions on PCR reactions. Seven stool concentration techniques were compared. The results showed that the most efficient concentration method for stool sample preparation for detection of G. intestinalis by PCR is centrifugal flotation with Percoll (with saturated NaNO₃ as the flotation solution). This method is relatively inexpensive, less labor-intensive, and suitable for epidemiological monitoring and clinical investigations.

Potential role of beavers (Castor fiber) in contamination of water in the Masurian Lake District (north-eastern Poland) with protozoan parasites Cryptosporidium spp. and Giardia duodenalis

51%

Sroka J. , Gizejewski Z. , Wojcik-Fatla A. , Stojecki K. , Bilska-Zajac E. , Dutkiewicz J. , Cencek T. , Karamon J. , Zajac V. , Kusyk P. , Dabrowska J. , Kochanowski M.

nr 2

The purpose of this study was to assess the possible influence of beavers on the contamination of lake water with zoonotic parasites Giardia duodenalis and Cryptosporidium spp., with respect to the risk to human health. A total of 79 water samples were taken around the habitats of beavers from 14 localities situated in the recreational Masurian Lake District (north-eastern Poland). Water was sampled in the spring and autumn seasons, at different distances from beavers' lodges (0-2, 10, 30, and 50 m). The samples were examined for the presence of (oo)cysts of zoonotic protozoa Giardia duodenalis and Cryptosporidium spp. by direct fluorescence assay (DFA) and by nested and real time PCR. By DFA, the presence of Giardia cysts was found in 36 samples (45.6%) and the presence of Cryptosporidium oocysts in 26 samples (32.9%). Numbers of Giardia cysts, Cryptosporidium oocysts, and summarised (oo)cysts of both parasites showed a significant variation depending on locality. The numbers of Giardia cysts significantly decreased with the distance from beavers' lodges while the numbers of Cryptosporidium oocy sts did not show such dependence. The amount of Giardia cysts in samples collected in spring was approximately 3 tiir.es higher than in autumn. Conversely , a larger number of Cryptosporidium oocy sts were detected in samples collected in autum than in spring. By PCR, Giardia DNA was found in 38 samples (48.1%) whereas DNA of Cryptosporidium was found in on samples (8.9%). Eleven Giardia isolates were subjected to phylogenetic analy sis by restriction fragment length poly morphism PCR or sequencing which evidenced their belonging to zoonotic assemblages: A (3 isolates) and B (8 isolates). In conclusion, water in the vicinity of beavers' lodges in the tested region was markedly contaminated with (oo)cysts of Giardia duodenalis and Cryptosporidium spp., which confirms the potential role of beavers as a reservoir of these parasites and indicates a need for implementation of appropriate preventive measures to protect tourists' health.