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EN
Extensive use of chromate compounds in the last few decades has resulted in contamination of our environment. In the present study we have investigated the effects of two different concentrations (10, 20 µg ml⁻¹) of chromium salts (CrCl₃, K₂CrO₄ K₂Cr₂O₇) on the growth of Zea mays L. As concentrations of chromium salts (CrCl₃, K₂CrO₄, K₂Cr₂O₇) increased, there was a significant decrease in seed germination ( 10-24%), shoot length (6-29%), root length ( 11 -33%), seedling length ( 16-24%), fresh weight of seedlings (17-67%) and increase in dry weight per seedling (3-15%), chromium content, acid phosphatases content (215-707%), and peroxidases activity (129-200%) of Zea mays plants compared to control treatment. In all treatments, the effect of hexavalent salts (K₂CrO₄ andK₂Cr₂O₇) was more severe on plant growth compared to trivalent Cr salts (CrCl₃). Zea mays plants have the ability to accumulate various chromium salts in their tissues and thus help to remediate the polluted soil.
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2012
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tom 34
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nr 6
EN
Synthetic seeds technology is a potential tool for an efficient and cost-effective clonal propagation system. In the present study, synthetic seeds were produced by encapsulating nodal segments (synthetic or synseeds) of Ruta graveolens in calcium alginate gel. The best gel complex was achieved using 3 % sodium alginate and 100 mM Cal₂.2H₂O. Maximum conversion response of synthetic seeds into plantlets was obtained on MS medium supplemented with 10 µM 6-benzyladenine (BA) and 2.5 µM α-naphthalene acetic acid (NAA). Encapsulated nodal segments could be stored at low temperature (4 °C) up to 4 weeks with a survival frequency of 86.7 %. The regenerated shoots rooted on MS medium containing 0.5 µM indole-3-butyric acid (IBA). Well-developed plantlets with proper root and shoot system from encapsulated nodal segments were hardened off successfully with 90 % survival rate. The high frequency of plant re-growth (conversion) from alginate-coated nodal segments coupled with high viability percentage after 4 weeks of storage is highly encouraging for the exchange of R. graveolens genetic resources.
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