Wyniki wyszukiwania - Biblioteka Nauki

1

Intercompartmental differences between cytosol and mitochondria in their respective antioxidative responses and lipid peroxidation levels in acid rain stress

100%

Wyrwicka A. , Sklodowska M.

|

nr 04

EN

Relatively little is known about the direct influence of acid rain (AR) on pro- and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria have not been studied before. Aboveground parts of plants were treated with different pH variants of AR and oxidative damages (lipid peroxidation) as well as antioxidative enzyme activities (superoxide dismutase, SOD; ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of oxidative damages was closely connected with the cell compartment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxide formation was observed in the mitochondrial fraction. In both cell compartments SOD activity did not change significantly. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defence of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.

2

Antioxidative enzymes activity and lipid peroxidation in cucumber cytosol and mitochondria after acid rain stress

100%

Wyrwicka A. , Sklodowska M.

|

nr 1 Suppl.

3

Antioxidative responses of cytosol and mitochondria to acid rain of different pH value

100%

Wyrwicka A. , Sklodowska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2008

|

tom 524

EN

Relatively little is known about the direct influence of acid rain (AR) on pro-and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria were not studied before. Aboveground parts of plants treated with different pH variants of AR and prooxidative changes (lipid peroxidation) as well as antioxidative enzyme activities (ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of prooxidative alterations was closely connected with the cell com partment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxides formation was observed in the mitochondrial fraction. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defense of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.

PL

Antropogeniczna kwaśna depozycja - deszcz, mgła i śnieg - występująca na znacznych obszarach Europy, Azji oraz Ameryki Północnej, jest czynnikiem stresowym ograniczającym plonowanie roślin. Wpływ kwaśnego deszczu na rozwój roślin jest przedmiotem badań prowadzonych od wielu lat, niewiele jest jednak danych dotyczących bezpośredniego wpływu kwaśnego deszczu na zmiany pro- i antyoksydacyjne w komórkach roślinnych. Nie analizowano różnic w odpowiedzi antyoksydacyjnej poszczególnych kompartmantów komórkowych. Przedmiotem prezentowanych badań były zmiany prooksydacyjne (nadtlenki lipidowe, TBARS), a także zmiany aktywności enzymów antyoksydacyjnych (peroksydaza askorbinianowa, APx; peroksydaza glutationowa, GSH-Px) we frakcji cytozolowej i mitochondrialnej liści roślin ogórka (Cucumis sativus L.), którego części nadziemne jednokrotnie traktowano kwaśnym deszczem o zróżnicowanej wartości pH. Charakter zmian prooksydacyjnych oraz aktywnosć enzymów antyoksydacyjnych był zależny od kompartmentu komórkowego, a także od pH kwaśnego deszczu i czasu po potraktowaniu. Aktywność obydwu enzymów wzrastała intensywniej w cytozolu. Natomiast, silny wzrost stężenia nadtlenków lipidowych był obserwowany we frakcji mitochondrialnej. Uzyskane wyniki sugerują, że mitochondria liści ogórka są bardziej wrażliwym na działanie kwaśnego deszczu kompartmentem niż cytozol. Odpowiedź antyoksydacyjna frakcji cytozolowej wydaje się zapewniać dostateczną ochronę przed działaniem stresu oksydacyjnego wywołanego kwaśnym deszczem.

4

Phytoremediation techniques of wastewater treatment

80%

Materac M. , Wyrwicka A. , Sobiecka E.

Environmental Biotechnology

|

2015

|

tom Vol. 11, No. 1

10--13

EN

Phytoremediation is a process of waste utilization and conversion by plants which includes such techniques as: phytoextraction, phytodegradation, phytovolatilization, rhizofiltration (phytofiltration) and phytostabilization. This review provides definitions and brief description of phytoremediation techniques and gives examples of their successful use.

5

Issues of biological and agricultural treatment of municipal sewage sludge

61%

Urbaniak M. , Wyrwicka A. , Kiedrzyńska E. , Staniak S. , Gałązka A. , Tołoczko W. , Siebielec G.

|

tom no. 12

35--47

EN

Increase of sewage sludge production in Poland requires undertaking specific methods for their utilization and disposal. The reason is that sewage sludge, in addition to being rich in organic matter, and biogenic compounds such as nitrogen and phosphorus, which are beneficial from an agricultural point of view, also contain heavy metals, toxic organic pollutants such as persistent organic pollutants and polycyclic aromatic hydrocarbons, inorganic compounds such as silicates and clay, and pathogenic bacteria and other microbial contaminants. This implies problems with further use of such polluted sludge as fertilizer in agriculture. The aim of this paper is to analyze the possibilities and limitations of natural management of sewage sludge, taking into account their effects on the restoration and conservation of organic matter in the soil and the yielding of plants. In addition, the main methods of treatment of polluted sludge before introducing it into the environment are considered.